miR-210-3p 通过靶向ATG7 抑制人膀胱癌细胞J82的自噬和诱导凋亡

doi:10.13418/j.issn.1001-165x.2018.06.004

Abstract

Abstract:

Objective　This paper mainly explored the regulatory mechanism of miR-210-3p in bladder cancer progression in J82 cell.　Methods　Quantitative real-time PCR (qRT-PCR) was applied to detect the expression of miR-210-3p and its target gene autophagy related gene 7 (ATG7) in J82 cells. The luciferase reporter assay was conducted to verify the biological relationship between miR-210-3p and ATG7. The biological functions of miR-210-3p and ATG7 in J82 cells were studied by in vitro experiments (CCK-8, hoechst staining, and Western blot). Results miR-210-3p expression significantly reduced the ATG7 expression level. Bioinformatics prediction and luciferase reporter assay demonstrated that miR-23-3p inhibited ATG7 by directly binding to ATG7 3’UTR. In J82 cells, miR-210-3p overexpression inhibited ATG7 expression, cell autophagy and cell proliferation, and induced cell apoptosis.　Conclusions　mir-210-3p can inhibit J82 cell proliferation, autophagy and promote apoptosis through negative regulation of ATG7, thereby promoting the death of cell J82 cells. Therefore, inhibition of autophagy in bladder cancer is essential for the development of autophagy targeting therapy for bladder cancer. This study further supports the mechanism of miR-210-3p in regulation of bladder cancer.

Key words: , Bladder cancer, 　miR-210-3p, 　Autophagy-related gene 7, 　Autophagy

HAN Xing-tao, YANG Ling-bo, WEI Peng-tao, LI Xiao-hui, SUN Jian-tao, YANG Jin-jian. miR-210-3p suppresses autophagy and promotes apoptosis by targeting autophagy-related gene 7 in bladder cancer J82 cells[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(6): 615-620.

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miR-210-3p suppresses autophagy and promotes apoptosis by targeting autophagy-related gene 7 in bladder cancer J82 cells

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