载ADM-PLGA纳米微球的ADM-PLGA-NHAC制备及其对人骨肉瘤MG63细胞的抑制作用

doi:10.13418/j.issn.1001-165x.2014.05.018

中国临床解剖学杂志 ›› 2014, Vol. 32 ›› Issue (5): 585-589.doi: 10.13418/j.issn.1001-165x.2014.05.018

载ADM-PLGA纳米微球的ADM-PLGA-NHAC制备及其对人骨肉瘤MG63细胞的抑制作用

杨联军，　荣子杰，　张赞杰，　伍国峰，　张西兵，　王健，　朱立新

南方医科大学珠江医院骨科中心，广州 510282

收稿日期:2014-01-20 出版日期:2014-09-25 发布日期:2014-10-14
通讯作者: 朱立新, 教授，主任医师，Zhulixin1966@163. com E-mail:lianjunyang1988@qq.com
作者简介:杨联军（1988-）,男，广东人，医学硕士，主要从事骨组织工程与脊柱外科，Tel: 13760788964
基金资助:
广州市科技民生科技重大专项（2011Y2-00023-2）

Synthesis and characterization of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres and observation of its inhibition on MG63 cells

YANG Lian-jun, RONG Zi-jie, ZHANG Zan-jie, WU Guo-feng, ZHANG Xi-bing, WANG jian, ZHU Li-xin

Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

Received:2014-01-20 Online:2014-09-25 Published:2014-10-14

摘要/Abstract

摘要：

目的　研制载阿霉素(ADM)的聚乳酸-羟基乙酸共聚物(PLGA)纳米微球的纳米羟基磷灰石/胶原复合支架（ADM-PLGA-NHAC），研究其性质及体外释药特点，探讨其体外抑制人骨肉瘤MG63细胞的作用，为骨肉瘤的治疗提供新策略。方法　以纳米羟基磷灰石及胶原为原料制备纳米羟基磷灰石/胶原支架并在其中加载ADM-PLGA纳米微球, 通过扫描电子显微镜、体外释放行为等手段评价载药支架材料的性能。以CCK8法、活-死染色评价该复合支架的浸提液在体外对人骨肉瘤MG63细胞株的抗肿瘤活性。结果　复合支架的孔径多在100~200 μm，孔隙率约为82%，微球与支架间结合较为紧密。复合支架具有良好的缓释特性，28 d内能持续缓慢释放阿霉素。复合支架的浸提液对骨肉瘤MG63细胞生长有明显的抑制作用。结论　制备的ADM-PLGA-NHAC复合支架具有良好的药物缓释特性及抗肿瘤效应，是一种具有良好应用前景的抗骨肉瘤骨修复材料。

关键词: 羟基磷灰石, 胶原, 阿霉素, 纳米微球, 骨肉瘤, 复合支架

Abstract:

Objective　To study preparation and characteristics of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres(ADM-PLGA-NHAC) and observe its in-vitro anti-tumor effect. Method　 ADM-PLGA-NHAC were prepared by freezing and lyophylization of composite solution.The characteristic and morphology of the scaffolds were observed by scanning electron microscope, and the release behavior of ADM was investigated in vitro. The antitumor activities of ADM-PLGA-NHAC against human MG63 cell lines were detected by CCK8 colorimetric assay and live/dead cell staining.　Result　The ADM-PLGA microspheres couldbe uniformly distributed in nano-hydroxyapatite/collagen scaffolds to form ADM-PLGA-NHAC scaffolds, which had 100-200 μm pore diameter and over 82% porosity. The release study indicated that the ADM-PLGA-NHAC show sustained release behavior in vitro. The leaching liquor of ADM-PLGA-NHAC could inhibit MG63 cell lines proliferation in a dose-dependent manner.　Conclusion　The ADM-PLGA-NHAC shows sustained release characteristics and anti-tumor effect, and it will have a wide application prospect in the fields of osteosarcoma.

Key words: Hydroxyapatite, Collagen, Adriamycin, Nanoparticles, Osteosarcoma, Porous scaffold

中图分类号:

R318.08

杨联军, 荣子杰, 张赞杰, 伍国峰, 张西兵, 王健, 朱立新. 载ADM-PLGA纳米微球的ADM-PLGA-NHAC制备及其对人骨肉瘤MG63细胞的抑制作用[J]. 中国临床解剖学杂志, 2014, 32(5): 585-589.

YANG Lian-Jun, RONG Zi-Jie, ZHANG Zan-Jie, WU Guo-Feng, ZHANG Xi-Bing, WANG Jian, SHU Li-Xin. Synthesis and characterization of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres and observation of its inhibition on MG63 cells[J]. Chinese Journal of Clinical Anatomy, 2014, 32(5): 585-589.

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载ADM-PLGA纳米微球的ADM-PLGA-NHAC制备及其对人骨肉瘤MG63细胞的抑制作用

Synthesis and characterization of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres and observation of its inhibition on MG63 cells

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