诱导型一氧化氮合酶对骨骼肌损伤再生的影响

doi:10.13418/j.issn.1001-165x.2015.05.013

中国临床解剖学杂志 ›› 2015, Vol. 33 ›› Issue (5): 545-548.doi: 10.13418/j.issn.1001-165x.2015.05.013

诱导型一氧化氮合酶对骨骼肌损伤再生的影响

赖桂华¹，　余磊²，　张翔³

1.蚌埠医学院人体解剖学教研室，安徽蚌埠 233030； 2.南方医科大学人体解剖学教研室，广东省组织构建
与检测重点实验室，广州 510515； 3 广州医科大学中华生物医学工程杂志编辑部，广州 511436

收稿日期:2015-04-29 出版日期:2015-09-25 发布日期:2015-10-13
作者简介:赖桂华（1982-），男，江西广昌人，硕士，讲师，研究方向：临床应用解剖学研究，骨骼肌的损伤修复及再生研究，E-mail: 357712637@qq.com
基金资助:
安徽省高等学校省级自然科学研究项目一般项目（KJ2013Z216）；安徽省重点实验室、工程中心（蚌埠医学院）开放课题计划重点项目（BYKL1401ZD）

Role of iNOS during wound healing of skeletal muscle

LAI Gui-hua¹, YU Lei², ZHANG Xiang³

1.Department of Human Anatomy, Bengbu Medical College, Bengbu, Anhui233030,China; 2.Department of Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection,Guangzhou510515,China; 3.Editorial Department of Journal of Modern Clinical Medical Bioengineering, Guangzhou Medical University, Guangzhou 511436,China

Received:2015-04-29 Online:2015-09-25 Published:2015-10-13

摘要/Abstract

摘要：

目的　观察骨骼肌损伤再生中的细胞形态学变化和损伤后诱导型一氧化氮合酶（inducible nitric oxide synthase, iNOS）的表达水平；观察巨噬细胞上清液对iNOS表达水平和骨骼肌再生的影响。方法制备SD大鼠骨骼肌损伤模型，损伤3 d后获取骨骼肌，进行HE染色、iNOS免疫组化分析；利用原代培养的巨噬细胞上清液处理小鼠C2C12成肌细胞株，RT-PCR技术探讨巨噬细胞和成肌细胞相互作用的分子机制。结果 HE染色可见，骨骼肌损伤处有新的血管产生并进入损伤的骨骼肌内；损伤部位iNOS表达强阳性，生发区iNOS表达呈阳性，正常与损伤细胞表达差异明显；巨噬细胞上清液处理小鼠C2C12成肌细胞株能够促进iNOS和成肌细胞因子MyoD的表达。结论骨骼肌损伤过程中，iNOS的表达增加能够促进骨骼肌的再生，加快骨骼肌的损伤修复。

关键词: 骨骼肌, 损伤再生, iNOS, 血管生成

Abstract:

Objective　To observe the morphological changes and iNOS expression at cellular damaged zone during the wound healing of denervated muscle; To determine the affection of the expression of iNOS induced by supernatant of macrophages.　Methods　The damaged denervated muscle, three days after the damaged muscle model of rat was established, was harvested for H.E and immunohistochemical staining of iNOS. The cultured C2C12 cells were treated with supernatant of macrophages. The treated C2C12 cells were analyzed using RT-PCR method.　Results　New capillaries near the damaged muscle and high expression of iNOS could be observed. The expression of iNOS was different between the damaged and undamaged zones in muscle cells. The supernatant of macrophages could induce the expression of iNOS and MyoD genes. Conclusion　The high expression of iNOS can accelerate the regeneration of denervated muscle during wound healing.

Key words: Skeletal muscle, Wound healing, iNOS, Angiogenesis

赖桂华，余磊，张翔. 诱导型一氧化氮合酶对骨骼肌损伤再生的影响[J]. 中国临床解剖学杂志, 2015, 33(5): 545-548.

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诱导型一氧化氮合酶对骨骼肌损伤再生的影响

Role of iNOS during wound healing of skeletal muscle

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