大鼠纤维化心肌组织中神经钙黏附素蛋白的表达变化

doi:10.13418/j.issn.1001-165x.2016.01.020

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (1): 78-82.doi: 10.13418/j.issn.1001-165x.2016.01.020

大鼠纤维化心肌组织中神经钙黏附素蛋白的表达变化

穆灵敏¹，　闫晓晓¹，　郑辉²，　张光谋³，　郭志坤⁴

1.新乡医学院形态学实验室；　2.新乡医学院第三附属医院；　3.新乡医学院生命科学技术学院细胞工程教研室；
4.河南省高等学校组织再生重点开放实验室，新乡 453003

收稿日期:2015-06-06 出版日期:2016-01-25 发布日期:2016-01-28
作者简介:穆灵敏（1974-），女，实验师，硕士，主要从事心脏发育研究，Tel：13598710169，E-mail: mulingmin736@163.com
基金资助:
河南省科技厅项目(142102310045)；新乡市科技局项目(ZG13023)

Influence of neural cadherin protein expression on myocardial fibrosis in rats

MU Ling-min¹, YAN Xiao-xiao¹, ZHENG Hui², ZHANG Guang-mou³ , GUO ZHi-kun⁴

1.Morphologial Laboratory of Xinxiang Medical College, 2. Affiliated Hospital of Third of Xinxiang Medical College, 3.Cell Engineering Department of Life Science and Technique College, Xinxiang Medical College； 4. The key Laboratory for Tissue Regeneration of Henan Universities, Xinxiang 453003, China

Received:2015-06-06 Online:2016-01-25 Published:2016-01-28

摘要/Abstract

摘要：

目的　研究异丙肾上腺素致大鼠心肌纤维化中神经钙黏附素（N-cadherin）蛋白在心肌组织中的表达，为探讨心肌纤维化的信号转导机制和逆转心肌纤维化提供形态学资料。方法　健康成年SD 大鼠注射ISO，造成心肌纤维化模型；取心肌组织，常规石蜡切片，Masson 染色，观察心肌组织胶原纤维改变；免疫组织化学和免疫荧光染色检测N-cadherin 的表达及分布；RT-PCR 方法检测大鼠心肌组织的N-cadherin 蛋白的mRNA 的表达变化；利用图像分析软件对N-cadherin 蛋白的免疫组化结果进行定量分析。结果　免疫组化和免疫荧光结果显示，实验组与对照组相比N-cadherin 蛋白表达位置存在动态变化，表达量无显著差异；RT-PCR 方法检测大鼠心肌组织的N-cadherin mRNA 的表达与免疫组化结果具有一致性。结论　N-cadherin 蛋白可能是维持心肌结构和生理功能所必需的。

关键词: 神经钙黏附素, N-cadherin, 免疫组织化学, 免疫荧光, 心肌纤维化

Abstract:

Objective　To investigate the expression of neural cadherin (N-cadherin) on isoproterenol induced myocardial fibrosis in rat, and provide morphological data for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy．Methods The rat models of myocardial hypertrophy were prepared by subcutaneous injection with isoprenaline (ISO) to healthy adult SD rats. The pathological changes of the myocardial collagen fiber were observed conventional paraffin section and Masson staining. The expression and distribution of N-cadherin was detected by immunohistoehemistry and immunofluorescence staining．Reverse transcription polymerase chain reaction was used to determine mRNA expression levels of N-cadherin in the myocardial tissues. Image analysis software was used to conduct quantitative analysis. Results Compared with the control group, the positive expression of N-cadherin protein in the experimental group had no significant difference. Furthermore, mRNA expression of N-cadherin in the myocardial tissues of rats was consistent with the immunohistoehemistry results. Conclusion The N-cadherin protein may be necessary for maintaining the structure and physiological function of myocardium.

Key words: Neural cadherin, Immunohistohemistry, Immunofluorescence staining, Myocardial fibrosis

穆灵敏,闫晓晓,郑辉,张光谋,郭志坤. 大鼠纤维化心肌组织中神经钙黏附素蛋白的表达变化[J]. 中国临床解剖学杂志, 2016, 34(1): 78-82.

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大鼠纤维化心肌组织中神经钙黏附素蛋白的表达变化

Influence of neural cadherin protein expression on myocardial fibrosis in rats

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