维生素C对大鼠背根神经节神经元突起生长的影响

doi:10.13418/j.issn.1001-165x.2016.03.015

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (3): 308-311.doi: 10.13418/j.issn.1001-165x.2016.03.015

维生素C对大鼠背根神经节神经元突起生长的影响

李媛媛¹，　文锦坤¹，　潘梦婕¹，　李莉霞¹，　王祥海¹，　钱长晖¹，　郭家松^1,2

1.南方医科大学基础医学院组织学与胚胎学教研室，广州 510515;
2.广东省组织工程构建与检测重点实验室，广州 510515

收稿日期:2016-02-23 出版日期:2016-05-25 发布日期:2016-06-17
通讯作者: 郭家松，教授，博士生导师，E-mail: jiasongguo@aliyun.com
作者简介:李媛媛(1990-)，女，福建人，在读硕士，Tel：18620576070，E-mail： yyuan1229@163.com
基金资助:
科技部973项目(2014CB542202)；国家自然科学基金 (81371354, 81571182); 广东省科技计划项目 (2015A020212024); 广东省自然科学基金 (S2013010014697)

The effect of vitamin C on the neurite outgrowth of dorsal root ganglionic neurons

LI Yuan-yuan¹, WEN Jin-kun¹, PAN Meng-jie¹, LI Li-xia ¹, WANG Xiang-hai¹, QIAN Chang-hui¹, GUO Jia-song ^1,2

1.Department of Histology and Embryology, Southern Medical University, Guangzhou 510515 China; 2.Key Laboratory of Tissue Construction and Detection of Guangdong Province, Guangzhou 510515,China

Received:2016-02-23 Online:2016-05-25 Published:2016-06-17

摘要/Abstract

摘要：

目的　研究维生素C（Vitamin C，Vit.C）对大鼠背根神经节神经元轴突生长的影响，为探讨神经再生新策略提供基础。方法　取新生SD大鼠背根神经节（dorsal root ganglion, DRG）进行神经元分离培养。在培养液中加入不同浓度的维生素C（0, 100, 200, 400 μmol/L）处理24 h后通过βIII tubulin, RhoA免疫组化以及鬼笔环肽染色对神经元突起的数量和长度、生长锥伪足数量、神经元胞体大小及其内RhoA表达强度进行分析。结果神经元突起的数量、长度，生长锥伪足的数量以及神经元胞体面积均随维生素C的浓度增高而增长，而神经元内RhoA表达强度则相应降低。结论维生素C可促进体外大鼠背根节神经元突起的生长，这可能与RhoA表达下调相关。

关键词: 维生素C, 神经元, 突起生长, RhoA

Abstract:

Objective　To study the effect of Vitamin C on the neurite outgrowth of dorsal root ganglionic neurons.　Methods　Neurons harvested from the dorsal root ganglion of newborn SD rat pups were cultured in a basic medium supplemented with a series of concentration of Vitamin C (0, 100, 200, 400μmol/L). 24 hours later, the cultures were immunostained with antibodies of βIII tubulin and RhoA. The βIII tubulin immunostained cultures were double stained with phalloidin. Then, the number and length of the neurites, number of pseudopodium in the growth cone of the cultured neurons, as well as the immunofluorence intensity of RhoA in the neuronal bodies were quantified.　Results　The number and length of the neurites, number of pseudopodium in the growth cone of the cultured neurons were all increased in Vit. C treated groups compared with the control group. Howerver， the RhoA level was decreased in the Vit. C treated groups.　Conclusion　Vit.C could promote the neurite outgrowth of cultured dorsal root ganglionic neurons which may result in reducing the expression of RhoA.

Key words: Vitamin C, Neuron, Neuriteougrowth, RhoA

李媛媛,文锦坤,潘梦婕,李莉霞,王祥海,钱长晖,郭家松. 维生素C对大鼠背根神经节神经元突起生长的影响[J]. 中国临床解剖学杂志, 2016, 34(3): 308-311.

LI Yuan-yuan, WEN Jin-kun, PAN Meng-jie, LI Li-xia,WANG Xiang-hai, QIAN Chang-hui, GUO Jia-song. The effect of vitamin C on the neurite outgrowth of dorsal root ganglionic neurons[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(3): 308-311.

参考文献

[1] Alagl AS, Bhat SG. Ascorbic acid: new role of an age-old micronutrient in the management of periodontal disease in older adults[J]. Geriatr Gerontol Int, 2015,15(3):241-254.
[2] Pardo B, Mena MA, Casarejos MJ, et al. Toxic effects of L-DOPA on mesencephalic cell cultures: protection with antioxidants[J]. Brain Res, 1995, 682 (1-2):133-143.
[3] Yan M, Yang M, Shao W, et al. High-dose ascorbic acid administration improves functional recovery in rats with spinal cord contusion injury[J]. Spinal Cord, 2014,52 (11):803-808.
[4] Lu R, Kallenborn-Gerhardt W, Geisslinger G, et al. Additive antinociceptive effects of a combination of vitamin C and vitamin E after peripheral nerve injury[J]. PLoS One, 2011,6 (12):e29240.
[5] Harrison FE, May JM. Vitamin C function in the brain: vital role of the ascorbate transporter SVCT2[J]. Free Radic Biol Med, 2009, 46 (6):719-730.
[6] Sango K, Kawakami E, Yanagisawa H, et al. Myelination in coculture of established neuronal and Schwann cell lines[J]. Histochem Cell Biol, 2012,137 (6):829-839.
[7] 韩泽民, 郭家松. 神经元内抑制神经再生的相关信号通路[J]. 生命科学, 2015, 27(9): 1120-1124.
[8] Chen J, Guo L, Zhang L, et al. Vitamin C modulates TET1 function during somatic cell reprogramming[J]. Nat Genet, 2013, 45 (12):1504-1509.
[9] Passage E, Norreel JC, Noack-Fraissignes P, et al. Ascorbic acid treatment corrects the phenotype of a mouse model of Charcot-Marie-Tooth disease[J]. Nat Med, 2004,10 (4):396-401.
[10] Engelhart MJ, Geerlings MI, Ruitenberg A, et al. Dietary intake of antioxidants and risk of Alzheimer disease[J]. Jama, 2002, 287(24):3223-3229.
[11] Fujita Y, Yamashita T. Axon growth inhibition by RhoA/ROCK in the central nervous system[J]. Front Neurosci, 2014, 8:338-349.
[12] Palazzolo G, Horvath P, Zenobi-Wong M. The flavonoid isoquercitrin promotes neurite elongation by reducing RhoA activity[J]. PloS one, 2012, 7(11):e49979.

[1]	林培敏, 刘燕, 刘婷, 尹颢霖, 张英. 腹内侧前额叶皮质锥体神经元参与选择性坐骨神经损伤小鼠疼痛和焦虑样行为调控的研究[J]. 中国临床解剖学杂志, 2023, 41(5): 557-564.
[2]	马晓维, 周喜燕, 李彬, 臧运华, 王群, 于淼. mir-325-3p激活RIPK3/TFEB信号通路减轻脑出血大鼠神经元损伤[J]. 中国临床解剖学杂志, 2023, 41(4): 422-427.
[3]	李丽楠, 丁汉琳, 刘金川. miR-181a调控sirt1通路抑制七氟烷诱导的海马神经元凋亡[J]. 中国临床解剖学杂志, 2023, 41(2): 200-206.
[4]	周立, 冉雪蕊, 王志勇. 下丘脑主要核团对机体血糖的影响[J]. 中国临床解剖学杂志, 2022, 40(4): 497-499.
[5]	李咪, 许嘉玮, 邹颖, 郭家松. 小鼠臂丛离断对脊髓运动神经元树突结构与形态退变的影响[J]. 中国临床解剖学杂志, 2022, 40(3): 277-285.
[6]	张家琪, 陈立, 李炯, 李素梅, 张吉凤, 滕继军, 郭国庆. Spastin促进海马神经元树突野的形成[J]. 中国临床解剖学杂志, 2020, 38(2): 176-182.
[7]	王雨, 陈传奇, 王文晟, 喻保军, 张义, 李丹, 陈龙菊. 葛根素对臂丛神经根性撕脱伤脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响[J]. 中国临床解剖学杂志, 2019, 37(5): 517-522.
[8]	范志浩,李媛媛,李莉霞,王祥海,潘梦婕,谭丹丹,文锦坤,钱长晖,李振林,郭家松. 维生素C对巨噬细胞增殖、迁移及吞噬功能的影响[J]. 中国临床解剖学杂志, 2017, 35(3): 266-270.
[9]	阚世廉,李桂石,詹海华,张建兵,马宁,李明新,赵嘉国. 大鼠周围神经移植修复后脊髓前角运动神经元变化的动态观察[J]. 中国临床解剖学杂志, 2016, 34(5): 534-539.
[10]	李素梅,李斌,张吉凤,谭明会,武凤鸣,郭国庆. RNA干扰CRMP 5抑制大鼠海马神经元突起生长[J]. 中国临床解剖学杂志, 2016, 34(3): 303-307.
[11]	李昂,晏芳,李振林,刘忠英,张琳,董为人,牛红心,王海红. 探讨阿糖胞苷在原代大鼠海马神经元培养中的纯化方法[J]. 中国临床解剖学杂志, 2015, 33(6): 681-684.
[12]	张丹丹,谭明会,叶永恒,陈克恩,李素梅,武凤鸣,郭国庆. CRMP4慢病毒载体的构建及其对神经元突起生长的影响[J]. 中国临床解剖学杂志, 2015, 33(3): 305-310.
[13]	许晓利, 李琴, 宋露, Ilya Bezprozvanny. Dimebon对小鼠中型多棘神经元NMDA激活电流的影响[J]. 中国临床解剖学杂志, 2015, 33(1): 89-92.
[14]	李红英, 张建杏, 王研, 于卫, 周国斌. 右美托咪定对大鼠神经元缺氧无糖损伤保护作用的实验研究[J]. 中国临床解剖学杂志, 2014, 32(4): 455-457.
[15]	朱继红, 王登科, 董克勤, 戴新文, 贺岭风. 三七总皂甙对脑出血大鼠的神经保护作用[J]. 中国临床解剖学杂志, 2014, 32(4): 442-445.

维生素C对大鼠背根神经节神经元突起生长的影响

The effect of vitamin C on the neurite outgrowth of dorsal root ganglionic neurons

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0