不同推拿手法对食蟹猴轻度颈动脉粥样硬化模型的影响

doi:10.13418/j.issn.1001-165x.2017.05.013

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (5): 543-547.doi: 10.13418/j.issn.1001-165x.2017.05.013

不同推拿手法对食蟹猴轻度颈动脉粥样硬化模型的影响

张磊^1,2，　李义凯²，　平瑞月³，　覃波¹，　周鑫¹，　汪国友¹，　关钛元¹，　曾胜强¹，　扶世杰¹

1.西南医科大学附属中医医院，四川泸州 646600；　2.南方医科大学中医药学院，广州 510515；
3.广州中医药大学，广州 510403

收稿日期:2017-03-29 出版日期:2017-09-25 发布日期:2017-10-30
通讯作者: 扶世杰，主任医师，硕士生导师，Tel:15283051308，E-mail:fushijieggj@126.com
作者简介:张磊（1987-），男，四川宜宾人，博士研究生，主治医师，研究方向：骨关节的基础和临床研究，E-mail：307501597@qq.com
基金资助:
四川省教育厅科研项目（17ZB0472）；泸州市科技局科技计划项目（2016-176-13）；西南医科大学-西南医科大学附属中医医院联合专项项目（2016-4-4）

The influence of massage manipulation on early carotid artery atherosclerotic plaque model of cynomolgus monkeys

ZHANG Lei ^1,2, PING Rui-yue³, LI Yi-kai², QIN Bo¹, ZHOU Xin¹, WANG Guo-you¹, GUAN Tai-yuan¹, ZENG Sheng-qiang¹, FU Shi-jie¹

1. Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Sichuan Province, Luzhou 646000; 2. School of Traditional Chinese Medicine, Southern Medical University, Guangdong Province, Guangzhou 510515; 3. Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510403

Received:2017-03-29 Online:2017-09-25 Published:2017-10-30

摘要/Abstract

摘要：

目的　探讨不同颈椎推拿手法对食蟹猴轻度颈动脉粥样硬化（CAS）模型稳定性的影响。方法　将18只食蟹猴建立轻度CAS模型，并以高脂饲料喂养8周，用彩超证实其造模成功。再将18只食蟹猴随机分为3组（每组6只），分别是：颈椎旋转组、推桥弓组和模型对照组。分别对颈椎旋转组和推桥弓组的食蟹猴进行相应的手法干预，共干预30 d，模型对照组不做特殊干预。最后将动物麻醉后处死，取颈总动脉分别进行HE染色、拉曼实验和压力实验。结果　HE染色可见颈总动脉血管内壁损伤严重，有斑块形成。拉曼试验中，3组均有明显的1450 及1660 脂质特征峰，但三组特征峰的相对强度差异无显著性意义（P > 0.05）。压力实验中，三组中食蟹猴颈动脉斑块破裂压力检测结果显示无显著性意义（P > 0.05）。结论　在食蟹猴轻度CAS病变中，短期内施用颈椎旋转手法和推桥弓手法并不会改变斑块的稳定性。

关键词: 颈动脉粥样硬化斑块, 　内膜损伤, 　拉曼实验, 　压力实验

Abstract:

Objective　To explore the influence of different cervical massage manipulation on the stability of early carotid artery atherosclerosis(CAS) model of cynomolgus monkeys. Methods 18 cynomolgus monkeys were developed early CAS model, and were fed with a high-fat diet for 8 weeks. The success of model was confirmed by Color Doppler Ultrasound. Then all monkeys were divided into three groups randomly: the Cervical Rotatory Manipulation(CRM) group, the Manipulation of Pushing on Qiaogong(MPQ) group and the control model group. The animals of the CRM group and the MPQ group underwent relevant interventions respectively for 30 days, but the control model group was not given any intervention. Finally all animals were euthanized, and the common carotid artery underwent HE staining, Raman test, pressure test. Results HE staining showed that the intima of common carotid artery were severely injured, and there was formation of CAS plaques. In Raman test, lipid characteristic peaks on 1450 and 1660 were obvious in 3 groups. However, there was no significant difference on the relative intensity of the 3 groups (P> 0.05). In pressure test, there was no significant difference on the results of fracture pressure of CAS plaques in 3 groups(P>0.05). Conclusion In the early stage of CAS in cynomolgus monkeys, the application of CRM and MPQ in short term have no impact on the stability of plaques.

Key words: Carotid artery atherosclerosis, 　Intimal injury, 　Raman test, 　Pressure test

张磊,李义凯,平瑞月,覃波,周鑫,汪国友,关钛元,曾胜强,扶世杰. 不同推拿手法对食蟹猴轻度颈动脉粥样硬化模型的影响[J]. 中国临床解剖学杂志, 2017, 35(5): 543-547.

ZHANG Lei, PING Rui-yue, LI Yi-kai, QIN Bo, ZHOU Xin, WANG Guo-you, GUAN Tai-yuan, ZENG Sheng-qiang, FU Shi-jie. The influence of massage manipulation on early carotid artery atherosclerotic plaque model of cynomolgus monkeys[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(5): 543-547.

参考文献

[1] Stoll G, Bendszus M. Inflammation and atherosclerosis: novel insights into plaque formation and destabilization [J]. Stroke, 2006, 37(7): 1923-1932.
[2] Gleissner CA. Translational atherosclerosis research: From experimental models to coronary artery disease in humans [J]. Atherosclerosis, 2016, 248: 110-116.
[3] Li Y, C G Z, Wang XH, et al. Progression of atherosclerosis in ApoE-knockout mice fed on a high-fat diet [J]. Eur Rev Med Pharmacol Sci, 2016, 20(18): 3863-3867.
[4] 孙晓云. 颈动脉粥样硬化的流行病学研究[J]. 中国医药指南, 2011, 9(5): 65-67.
[5] 黄雨晴, 魏学标, 詹嘉欣, 等. 原发性高血压患者静息心率与颈动脉粥样硬化的关系 [J]. 中华临床医师杂志, 2014, 8(13): 1-4.
[6] 何宗宝, 吕有魁, 陈东昌. 颈椎性血压异常国内研究概况 [J]. 中华物理医学与康复杂志, 2006, 28(9): 637-639.
[7] Peng B, Pang X, Li D, et al. Cervical spondylosis and hypertension[J]. Medicine, 2015, 94(10): e618.
[8] 郭剑华, 马善治, 刘渝松, 等. 中医优势病种颈椎病综合治疗优化方案临床研究 [J]. 实用中医药杂志, 2011, 8(8): 517-521.
[9] 邬黎平, 陈永源, 黄远翘, 等. 不同体位下颈椎拔伸旋转手法治疗神经根型颈椎病的疗效对比研究 [J]. 中国康复医学杂志, 2014, 29(1): 47-50.
[10] 冯跃, 杨洁, 杨馨. 桥弓穴源流简考 [J]. 吉林中医药, 2010, 30(6): 542-543.
[11] Riou L M, Broisat A, Ghezzi C, et al. Effects of mechanical properties and atherosclerotic artery size on biomechanical plaque disruption-mouse versus human [J]. J Biomech, 2014, 47(4): 765-772.
[12] 金龙, 乔爱科. 颈动脉易损斑块的生物力学机制和破裂风险评价指标 [J]. 医用生物力学, 2016, 31(1): 89-94.
[13] 章成国, 张虹桥, 谢坚, 等. 缺血性脑血管病与颈动脉粥样硬化的关系 [J]. 中华神经科杂志, 2006, 39(12): 832-835.

[14] Paolo PLD, Napoli A, Anzidei M, et al. Comparison between dual-energy CT- angiography, MR-angiography and digital substraction angiography for the evaluation of carotid artery stenosis: A prospective study [J]. Bmc Vet Res, 2015, 11(1): 1-5.
[15] Kapourchali FR, Surendiran G, Chen L, et al. Animal models of atherosclerosis [J]. World Journal of Clinical Cases, 2014, 2(5): 126-132.
[16] Rekhter M D, Hicks G W, Brammer D W, et al. Animal model that mimics atherosclerotic plaque rupture [J]. Circulation Research, 1998, 83(7): 705-713.
[17] 陈文强, 张运. 动脉粥样硬化易损斑块的动物模型和检测技术 [J]. 中国动脉硬化杂志, 2016, 24(7): 649-656.
[18] 王建辉, 李磊, 刘建勋. 动脉粥样硬化易损斑块动物模型研究 [J]. 中药药理与临床, 2011, 27(4): 89-92.
[19] 乔俊文, 陆姮磊, 孙景军,等. 药物临床前安全性评价中食蟹猴常见自发性病变 [J]. 中国药理学与毒理学杂志, 2013, 27 (3): 548.
[20] Saul L, Josephs D H, Cutler K, et al. Comparative reactivity of human IgE to cynomolgus monkey and human effector cells and effects on IgE effector cell potency [J]. Mabs, 2014, 6(2): 509.
[21] Shen H, Liu T, Jiang H, et al. Cynomolgus monkey as a clinically relevant model to study transport involving renal organic cation transporters: in vitro and in vivo evaluation [J]. Drug Metab Dispos, 2016, 44(2): 238-249.
[22] Niu Y, Shen B, Cui Y, et al. Generation of gene-modified cynomolgus monkey via Cas9/ RNA-mediated gene targeting in one-cell embryos [J]. Cell, 2014, 156(4): 836.
[23] Wang C, Tian F, Zhou Y, et al. The incidence of cervical spondylosis decreases with aging in the elderly, and increases with aging in the young and adult population: a hospital-based clinical analysis[J]. Clin Interv Aging, 2016, 11: 47-53.
[24] Kalb S, Zaidi H A, Ribasnijkerk J C, et al. Persistent outpatient hypertension is independently associated with spinal cord dysfunction and imaging characteristics of spinal cord damage among patients with cervical spondylosis [J]. World Neurosurg, 2015, 84(2): 351-357.
[25] Lotufo PA, Pereira AC, Vasconcellos PS, et al. Resistant hypertension: risk factors, subclinical atherosclerosis, and comorbidities among adults-the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) [J]. J Clin Hypertens, 2015, 17(1): 74-80.
[26] 楚福明, 张中, 刘剑伟, 等. 旋转手法与常规手法治疗神经根型颈椎病疗效分析 [J]. 中国中医骨伤科杂志, 2009, 17(3): 36-37.
[27] 王秋梨, 黄锦萍, 梁亮标. 旋转手法治疗椎动脉型颈椎病效果观察 [J]. 实用中医药杂志, 2016, 32(10): 1009.
[28] 黄谷, 蔡黎, 沈晔, 等. 抹桥弓法联合按揉风池穴干预轻中度高血压的临床研究 [J]. 中西医结合心脑血管病杂志, 2016, 14(8): 901-903.

不同推拿手法对食蟹猴轻度颈动脉粥样硬化模型的影响

The influence of massage manipulation on early carotid artery atherosclerotic plaque model of cynomolgus monkeys

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