依达拉奉联合灯盏花素对脑缺血大鼠大脑皮质MCP-1的影响

doi:10.13418/j.issn.1001-165x.2018.01.010

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (1): 38-44.doi: 10.13418/j.issn.1001-165x.2018.01.010

依达拉奉联合灯盏花素对脑缺血大鼠大脑皮质MCP-1的影响

李璠¹，　陈伟伟²，　赵晓姝³，　曾洪艳³，　韩宏²，　宋咏丽²，　袁云²，　吴春云²

1.昆明医科大学科研实验中心；　2. 昆明医科大学人体解剖学与组织胚胎学系，昆明 650500；
3. 昆明医科大学海源学院，昆明 651701

收稿日期:2017-09-13 出版日期:2018-01-25 发布日期:2018-03-06
通讯作者: 吴春云，教授，博士生导师，Tel:0871-65922850, E-mail:wuchunyunkm@163.com; 袁云，副教授，Tel:0871-65922850, E-mail:yunyuankm@126.com
作者简介:李璠(1984-), 女，云南临沧人，硕士，助理实验师，主要从事基础医学及分子生物学实验技术工作，Tel:0871-65922944，E-mail:282067107@qq.com
基金资助:
云省应用基础研究计划重点项目（2015FA020）；国家自然科学基金（31260254）

The effects of edaravone combined with breviscapine treatment on MCP-1 expression in the cerebral cortex after focal cerebral ischemia in rats

LI Fan¹, CHEN Wei-wei², ZHAO Xiao-shu³, ZENG Hong-yan³, HAN Hong², SONG Yong-li², YUAN Yun², WU Chun-yun²

1. Department of Experiment Center for Medical Science Research; 2. Department of Anatomy/Histology and Embryology, Kunming Medical University, Kunming 650500；3. Kunming Medical University Haiyuan College, Kunming 651701, China

Received:2017-09-13 Online:2018-01-25 Published:2018-03-06

摘要/Abstract

摘要：

目的　观察大鼠脑缺血（MCAO）后大脑皮质MCP-1的表达变化以及用依达拉奉联合灯盏花素干预后对MCP-1表达的影响。方法　复制大鼠大脑中动脉闭塞（MCAO）模型，应用RT-PCR、Western blot和免疫荧光技术检测大鼠MCAO及药物干预后MCP-1的表达变化。结果　RT-PCR显示大鼠MCAO后大脑皮质MCP-1 mRNA的表达比对照组显著上升（P<0.05），12 h达高峰，与1 d、3 d及1周组比有显著差异（P<0.05）；给予两种药物联合处理后，mRNA表达显著降低，与对照组及单独用药组相比均有显著差异（P<0.05）。 Western blot显示MCAO 1 d、3 d、1周大脑皮质MCP-1蛋白的表达显著增强，与对照组比有显著差异（P<0.05）；两种药物联合处理后，MCAO大鼠大脑皮质MCP-1蛋白表达明显减少，与对照组及单独用药相比均有显著差异（P<0.05）。免疫荧光染色显示MCAO大鼠缺血半暗带有大量激活小胶质细胞，一部分与MCP-1免疫阳性细胞有共表达，其中MCAO后1周组最明显。结论　大鼠局灶性脑缺血（MCAO）后，依达拉奉联合灯盏花素治疗能有效减少大脑皮质MCP-1的表达，效果优于两种药物单独使用。

关键词: 脑缺血, 　灯盏花素, 　依达拉奉, 　MCP-1, 　大鼠

Abstract:

Objective　This research investigated the expression of MCP-1 in cerebral cortex of rat following middle cerebral artery occlusion (MCAO) and edaravone combination with breviscapine as a treatment of MCP-1 expression.　Methods　Animal models of MCAO were established， and RT-PCR，Western blot and Immunohistochemistry were used to detect the expression of MCP-1 in cerebral cortex of rats after MCAO and drug intervention. Results The expression of MCP-1 mRNA were significantly enhanced after MCAO, and became most severe at 12 h, and the difference was significant (P<0.05). The expression of MCP-1 mRNA was markedly decreased compared with the saline group after drug treatment (P<0.05)； The combination of edaravone plus breviscapine was more effective than edaravone or breviscapine treatment alone (P<0.05). The expression of MCP-1 protein was significantly increased in 1d, 3d, 1 week following MCAO and stayed at a high level (P<0.05), and than obviously decreased compared with the saline group after drug treatment (P<0.05); the combination of edaravone plus breviscapine was more effective than edaravone or breviscapine alone (P<0.05) . Immunofluorescence staining showed that activated microglial cells was co-labeled with MCP-1 positive cells, and the number of MCP-1 positive cells was significantly increased after MCAO peaking at 1week. By the mean time, microglial cells did not exhibit MCP-1 positive cells in saline group.　Conclusion　Edaravone combination with breviscapine as a treatment could decrease the MCP-1 expression after cerebral ischemia, and the combination of edaravone plus breviscapine is more effective than edaravone or breviscapine alone.

Key words: Cerebral ischemia;　Breviscapine;　Edaravone;　Monocyte chemotactic protein 1, 　Rats

李璠,陈伟伟,赵晓姝,曾洪艳,韩宏,宋咏丽,袁云,吴春云. 依达拉奉联合灯盏花素对脑缺血大鼠大脑皮质MCP-1的影响[J]. 中国临床解剖学杂志, 2018, 36(1): 38-44.

LI Fan, CHEN Wei-wei, ZHAO Xiao-shu, ZENG Hong-yan, HAN Hong, SONG Yong-li, YUAN Yun, WU Chun-yun. The effects of edaravone combined with breviscapine treatment on MCP-1 expression in the cerebral cortex after focal cerebral ischemia in rats[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(1): 38-44.

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依达拉奉联合灯盏花素对脑缺血大鼠大脑皮质MCP-1的影响

The effects of edaravone combined with breviscapine treatment on MCP-1 expression in the cerebral cortex after focal cerebral ischemia in rats

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