关键词: 肌动蛋白重组, 　Activin A, 　MAPK信号通路, 　成纤维细胞

Abstract:

Objective　To explore whether Activin A regulate the filamentous actin of fibroblasts via MAPK signaling pathway.　Methods　Fibroblasts were Isolated and cultured from C57BL/6 rat skin which was born within 24 hours. The experiments were divided into PBS group, Activin A group, JNK specific inhibitor SP600125 group, ERK specific inhibitor SL327 group and p38 specific inhibitor SB202190 group. When cells were passaged for 3~5 generations, the total protein of cells was extracted, and the phosphorylation activity of JNK, ERK and p38 was detected by western blot. The changes of F-actin were observed by Phallotoxins staining.　Results　Activin A could induce the accumulation of F-actin in fibroblasts. After the use of JNK specific inhibitor SP600125, the F-actin aggregation of fibroblasts was completely inhibited. When the ERK specific inhibitor SL327 was used, the fibroblast F-actin aggregation was weakened, but not yet completely inhibited while P38 specific inhibitor SB202190 did not suppress the fibroblast F-actin aggregation.　Conclusion　 Activin A can induce the F-actin recombination of fibroblasts via JNK and ERK signaling pathway.

Key words: Filamentous actin recombination, 　Activin A, 　MAPK signaling pathway, 　Fibroblasts