VIP对LPS应激的小鼠成纤维细胞TREM-2表达的影响及其机制

doi:10.13418/j.issn.1001-165x.2018.04.010

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (4): 402-407.doi: 10.13418/j.issn.1001-165x.2018.04.010

VIP对LPS应激的小鼠成纤维细胞TREM-2表达的影响及其机制

李淑芬¹，　宋卓慧¹，　杨慧慧²，　刘永平²，　熊建兵²，　管茶香²，　孙国瑛^{2, 3}

1.长治医学院生理学教研室，山西长治 046000；　2.中南大学湘雅医学院生理学系，长沙 410078；
3.湖南师范大学医学院组织学与胚胎学教研室，长沙 410013

收稿日期:2018-05-07 出版日期:2018-07-25 发布日期:2018-08-21
通讯作者: 孙国瑛，硕士生导师，E-mail：sunguoying1029@hunnu.edu.cn
作者简介:李淑芬（1981-），山西长治人，硕士，讲师，主要研究方向为肺疾病的损伤与保护机制，E-mail：lsfddcy@163.com
基金资助:
国家自然科学基金（81670014）；湖南省自然科学基金(2018JJ3368)；中南大学博士后基金（160320001）；长治医学院科研启动基金项目（QDZ201518）

Effect of VIP on the expression of TREM-2 in mouse fibroblasts with LPS stress and its mechanism

LI Shu-fen¹, SONG Zhuo-hui¹, YANG Hui-hui², LIU Yong-ping², XIONG Jian-bing², GUAN Cha-xiang², SUN Guo-ying ^{2, 3}

1.Department of Physiology, Changzhi Medical College, Changzhi 046000, Shanxi Province, China; 2.Department of Physiology, Xiangya Medical School, Central South University, Changsha 410078, China; 3.Department of Histology and Embryology，School of Medicine, Hunan Normal University, Changsha 410013, China

Received:2018-05-07 Online:2018-07-25 Published:2018-08-21

摘要/Abstract

摘要：

目的　观察血管活性肠肽（vasoactive intestinal peptide，VIP）对脂多糖（lipopolysaccharides, LPS）应激的小鼠成纤维细胞髓样细胞表达触发受体-2（TREM-2）表达的影响，并初步探讨其信号转导通路。方法　利用LPS腹腔注射建立急性肺损伤（ALI）小鼠模型；采用VIP慢病毒气管滴注，qPCR检测肺组织TREM-2的表达。选用qPCR和流式细胞术检测VIP对LPS应激的小鼠成纤维细胞TREM- 2表达的影响；并观察PKC信号通路阻断剂（H-7）、PKA信号通路阻断剂（H- 89）、MAPK信号通路阻断剂（PD98059）和CaM信号通路阻断剂（W-7）对VIP调控TREM- 2表达的影响。结果　ALI时小鼠肺组织TREM-2 mRNA表达降低，而VIP可上调肺组织TREM- 2 mRNA的表达。LPS下调小鼠成纤维细胞TREM- 2 mRNA的表达，VIP可呈时间依赖性上调TREM- 2 mRNA的表达（0、3 、6 、12和24 h），且在6 h达到峰值；并呈剂量相关性上调TREM- 2 mRNA的表达（10-10、10-9、10-8和10-7 mol/L），以10-8 mol/L作用最明显。VIP对LPS应激6 h增加小鼠成纤维细胞TREM-2 mRNA和蛋白表达的效应可被H-7、PD98059以及W- 7所阻断。结论　LPS下调小鼠成纤维细胞TREM-2的表达，而VIP可上调LPS应激的小鼠成纤维细胞TREM- 2 mRNA的表达，其胞内信号转导途径可能与PKC、MAPK及CaM有关。

关键词: 急性肺损伤, 　髓样细胞表达的触发受体-2, 　血管活性肠肽, 　脂多糖, 　成纤维细胞

Abstract:

Objective　To observe the effect of VIP(vasoactive intestinal peptide) on TREM-2 expression in mouse fibroblasts induced by LPS(lipopolysaccharides) and to explore its possible signaling pathway. Methods　ALI animal model was established by intraperitoneal injection of LPS, and VIP lentivirus tracheal infusion was used, and the expression of TREM-2 in lung tissue was detected by qPCR. qPCR and flow cytometry were used to detect the effect of VIP on TREM-2 expression in LPS-induced fibroblasts, and to observe the effects of PKC signal pathway blocker (H-7), PKA signal pathway blocker (H-89), MAPK signal pathway blocker (PD98059) and CaM signal pathway blocker (W-7) on the regulation of VIP expression TREM-2.　Results　The TREM-2 mRNA expression of lung tissue in mice decreased in ALI, while VIP could increase TREM-2 mRNA expression in lung tissue. LPS down-regulated the expression of TREM-2 mRNA in mice fibroblast, and VIP up-regulated the expression of TREM-2 mRNA in a time-dependent manner (0 h, 3 h, 6 h, 12 h, 24 h), and reached the peak at 6 h. VIP dose related with the TREM-2 mRNA expression (10-10 mol/L, 10-9 mol/L, 10-8 mol/L, 10-7 mol/L). The up-regulation effect of VIP on TREM-2 mRNA and protein expression in mouse fibroblasts (stress by LPS for 6 h) could be blocked by H-7, PD98059, and W-7. 　Conclusion　LPS can down-regulate the TREM-2 expression of mouse fibroblasts, while VIP can up-regulate the expression of TREM-2 in LPS induced fibroblasts, and the intracellular signal transduction pathway may be related to PKC, MAPK and CaM.

Key words: Acute lung injury;　Triggering receptor expressed on myeloid cells-2;　Vasoactive intestinal peptide;　Lipopolysaccharide; , Fibroblast

李淑芬,宋卓慧,杨慧慧,刘永平,熊建兵,管茶香,孙国瑛. VIP对LPS应激的小鼠成纤维细胞TREM-2表达的影响及其机制[J]. 中国临床解剖学杂志, 2018, 36(4): 402-407.

LI Shu-fen, SONG Zhuo-hui, YANG Hui-hui, LIU Yong-ping, XIONG Jian-bing, GUAN Cha-xiang, SUN Guo-ying. Effect of VIP on the expression of TREM-2 in mouse fibroblasts with LPS stress and its mechanism[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(4): 402-407.

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VIP对LPS应激的小鼠成纤维细胞TREM-2表达的影响及其机制

Effect of VIP on the expression of TREM-2 in mouse fibroblasts with LPS stress and its mechanism

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