提高血流切应力对老年大鼠后肢侧支血管生长的作用

doi:10.13418/j.issn.1001-165x.2018.05.009

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (5): 527-531.doi: 10.13418/j.issn.1001-165x.2018.05.009

提高血流切应力对老年大鼠后肢侧支血管生长的作用

董丽萍^1,2，　刘光敏¹，　王加杰¹，　蔡维君¹

1. 中南大学基础医学院组织学与胚胎学系，长沙 410013； 2.长沙医学院人体解剖与组织胚胎学教研室，长沙 410219

收稿日期:2018-05-08 出版日期:2018-09-25 发布日期:2018-10-26
通讯作者: 蔡维君，研究员，Tel:15874099375，E-mail：caiweijun @csu.edu.cn
作者简介:董丽萍（1984-），讲师，硕士，研究方向：血管重建，E-mail:ddongliping@163.com
基金资助:
国家自然科学基金（81370248，81500377）；湖南省教育厅一般项目（16C0163）

The effect of elevated shear stress on collateral vessel growth of aged rats

DONG Li-ping ^1,2, LIU Guang-min¹, WANG Jia-jie¹, CAI Wei-jun¹

1. Department of Histology & Embryology, School of Basic Medicine, Central South University Changsha 410013, Hunan, China; 2. Department of Anatomy, Histology and Embryology, Changsha Medical University, Changsha 410219, Hunan, China

Received:2018-05-08 Online:2018-09-25 Published:2018-10-26

摘要/Abstract

摘要：

目的　利用老年大鼠股动脉结扎加股动静脉吻合和单纯股动脉结扎的侧支血管生长模型探讨提高血流切应力对老年大鼠侧支血管生长的作用。方法　老年SD大鼠（24月龄）30只，分为假手术组，结扎组和吻合组。结扎组大鼠行股动脉结扎手术，吻合组大鼠行股动脉结扎加股动静脉吻合手术。大鼠术后存活1周，每组5只大鼠取后肢带侧支血管的肌肉做冰冻切片用于免疫荧光组织化学法检测eNOS，Ki67和MMP2的表达；另外5只使用明胶四氧化三铅进行血管造影再用X线拍照观察侧支血管的生长情况。结果　与假手术组相比，结扎组的大鼠后肢侧支血管数量增多（P<0.01），Ki67阳性细胞数量增多（P<0.01），eNOS的表达显著增强（P<0.001），MMP2的表达也有所增强（P<0.05）。吻合组与结扎组相比，大鼠的后肢侧支血管数量进一步增多（P<0.01），Ki67阳性细胞数量明显增多（P<0.01），eNOS的表达显著增强（P<0.001），MMP2的表达也明显增强（P<0.01）。结论　提高血流切应力能促进老年大鼠缺血后肢侧支血管生长。

关键词: 老化, 　侧支血管, 　吻合

Abstract:

Objective　To investigate the effect of elevated shear stress on collateral vessel growth of aged rats by comparing the model induced by femoral artery ligation with femoral arteriovenous anastomosis and collateral vessel growth model with simple femoral artery ligation.　Methods　30 aged (24 months) rats were divided into sham group, ligation group and arteriovenous anastomosis group. The rats in the ligation group underwent femoral artery ligation, and the rats in the anastomosis group were subjected to femoral artery ligation and femoral arteriovenous anastomosis. The animals survived for 1 week after surgery. 5 rats in each group were taken to isolate the muscle with collateral vessels of the hindlimb for immunohistochemical for detecting expression of eNOS, Ki67 and MMP2; the other 5 rats were used for angiography, after which X-ray photography was performed to observe the growth of collateral vessels. Results　Compared with the sham operated group, the number of collateral vessels increased in the ligation group (P<0.01), the number of Ki67-positive cells increased (P<0.01), and so as the expression of eNOS (P<0.001) and MMP2 (P<0.05). Compared with the ligation group, the number of collateral vessels in femoral artery ligation with femoral arteriovenous anastomosis group was increased（P<0.01）, the number of Ki67-positive cells increased significantly (P<0.01), which was the same with the expression of eNOS (P<0.001) and MMP2 (P<0.01).　Conclusion　Elevated shear stress can promote the growth of collateral vessels in the ischemic hindlimb of aged rats.

Key words: Aging; , Collateral vessels; , Anastomosis

董丽萍,刘光敏,王加杰,蔡维君. 提高血流切应力对老年大鼠后肢侧支血管生长的作用[J]. 中国临床解剖学杂志, 2018, 36(5): 527-531.

DONG Li-ping,LIU Guang-min, WANG Jia-jie, CAI Wei-jun. The effect of elevated shear stress on collateral vessel growth of aged rats[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(5): 527-531.

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提高血流切应力对老年大鼠后肢侧支血管生长的作用

The effect of elevated shear stress on collateral vessel growth of aged rats

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