内源性雌激素调控脓毒症小鼠心肌L型钙离子通道α1C亚基的表达

doi:10.13418/j.issn.1001-165x.2019.05.009

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (5): 523-527.doi: 10.13418/j.issn.1001-165x.2019.05.009

内源性雌激素调控脓毒症小鼠心肌L型钙离子通道α1C亚基的表达

冯莹，　刘伯毅，　方志成，　陈黎，　陈伟，　郑翔

十堰市太和医院重症医学科，湖北医药学院附属医院，湖北十堰 442000

收稿日期:2019-02-15 出版日期:2019-09-25 发布日期:2019-09-25
通讯作者: 郑翔，Tel：0719-8801481；E-mail：syzxth0719@163.com
作者简介:冯莹（1989-），女，河南正阳人，主管技师，硕士，E-mail：fyhappy.hello@163.com
基金资助:
十堰市科学技术研究与开发项目（17Y11）

Endogenous estrogen regulates the expression of myocardial L-type calcium channel α1C subunit (CACNA1C) in septic mice

FENG Ying , LIU Bo-yi, FANG Zhi-cheng, CHEN Li, CHEN Wei, ZHENG Xiang

Department of Intensive Care Unit, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China

Received:2019-02-15 Online:2019-09-25 Published:2019-09-25

摘要/Abstract

摘要： 目的　探讨内源性雌激素对脓毒症小鼠心肌钙离子通道α1C亚基（CACNA1C）的影响。方法　雌性昆明小鼠行双侧卵巢摘除术，术后30 d利用盲肠结扎穿刺术（CLP）建立脓毒症小鼠模型，分为5组：脓毒症组（S组），去卵巢+脓毒症组（QS组），假手术组（C组），去卵巢+假手术组（QC组），去卵巢+苯甲酸雌二醇（QE组）。荧光定量PCR和Western blot检测造模后3 h，6 h，12 h，24 h左心室CACNA1C表达水平，免疫组化检测造模后12 h左心室CACNA1C表达及分布情况。结果　（1）QC组CACNA1C的表达水平显著高于C组，QE组CACNA1C的表达水平显著低于QC组。（2）CLP后3 h，S组CACNA1C表达显著高于对照组和其它时间点。（3）CLP后3 h、6 h、12 h和24 h，QS组CACNA1C表达均显著低于对照组，且各时间点之间无差异。结论　（1）双侧卵巢摘除后CACNA1C表达显著升高，双侧卵巢摘除后补给苯甲酸雌二醇可以逆转CACNA1C的升高。（2）正常小鼠CLP后3 h CACNA1C的表达出现短暂的升高，而双侧卵巢摘除的小鼠CLP后各时间点CACNA1C的表达均显著低于对照组。

关键词: , 雌激素；　L型钙离子通道；　CACNA1C；　脓毒症

Abstract: Objective　To investigate the effect of endogenous estrogen on myocardial L-type calcium channel α1C subunit (CACNA1C) in septic mice.　Methods　Female Kunming mice underwent bilateral ovarian ablation, a model of sepsis was established by cecal ligation puncture (CLP) 30 days after surgery. The experimental mice were divided into 5 groups: sepsis group (S group), ovariectomy+sepsis group (QS group), sham group (C group), ovariectomy+sham group (QC group), ovariectomy+estradiol benzoate (QE group). The expression levels of left ventricular CACNA1C were detected by real-time PCR and Western blot at 3 h, 6 h, 12 h, and 24 h after CLP. The expression and distribution of CACNA1C was detected by immunohistochemistry 3 h after CLP.　Results　The expression of CACNA1C in QC group was significantly higher than that in the C group, while the expression level of CACNA1C in the QE group was significantly lower than that in the QC group. At 3 h after CLP, the expression of CACNA1C in the S group was significantly higher than that in the control and other time points. At 3 h, 6 h, 12 h and 24 h after CLP, the expression of CACNA1C in the QS group was significantly lower than that in the control group, and there was no significant difference among the four time points.　Conclusion The expression of CACNA1C is significantly increased after bilateral ovarian ablation. Replenishment of estradiol benzoate after lateral ovarian ablation can reverse the increase of CACNA1C. The expression of CACNA1C is transiently increased at 3 h after CLP in normal mice, while the expression of CACNA1C at each time point after CLP in bilateral ovarian-extracted mice is significantly lower than that in the control group.

Key words: , Estrogen；　L- type calcium channel；　CACNA1C；　Sepsis

中图分类号:

R363.2

冯莹, 刘伯毅, 方志成, 陈黎, 陈伟, 郑翔. 内源性雌激素调控脓毒症小鼠心肌L型钙离子通道α1C亚基的表达[J]. 中国临床解剖学杂志, 2019, 37(5): 523-527.

FENG Ying , LIU Bo-yi, FANG Zhi-cheng, CHEN Li, CHEN Wei, ZHENG Xiang. Endogenous estrogen regulates the expression of myocardial L-type calcium channel α1C subunit (CACNA1C) in septic mice[J]. Chinese Journal of Clinical Anatomy, 2019, 37(5): 523-527.

参考文献 16

[1]	Rhodes A, Evans LE, Alhazzani W, et al. Surviving sepsis campaign: international guidelines for management of sepsis and septic shock: 2016[J]. Intensive Care Med, 2017, 43(3): 304-377.
[2]	Fleischmann C, Thomas-Rueddel DO, Hartmann M, et al. Hospital incidence and mortality rates of sepsis[J]. Dtsch Arztebl Int, 2016, 113(10): 159-166.
[3]	Martin L, Horst K, Chiazza F, et al. The synthetic antimicrobial peptide 19-2.5 attenuates septic cardiomyopathy and prevents down-regulation of SERCA2 in polymicrobial sepsis[J]. Sci Rep, 2016, 6: 37277.
[4]	Papp R, Bett GCL, Lis A, et al. Genomic upregulation of cardiac Cav1.2alpha and NCX1 by estrogen in women [J]. Biol Sex Differ, 2017, 8(1): 26.
[5]	May AK, Dossett LA, Norris PR, et al. Estradiol is associated with mortality in critically ill trauma and surgical patients[J]. Crit Care Med, 2008, 36(1): 62-68.
[6]	Sakr Y, Elia C, Mascia L, et al. The influence of gender on the epidemiology of and outcome from severe sepsis[J]. Crit Care, 2013, 17(2): R50.
[7]	Wu CC, Chang CY, Chang ST, et al. 17beta-estradiol accelerated renal tubule regeneration in male rats after ischemia/reperfusion-induced acute kidney injury[J]. Shock, 2016, 46(2): 158-163.
[8]	Saia RS, Garcia FM, Cárnio EC. Estradiol protects female rats against sepsis induced by Enterococcus faecalis improving leukocyte bactericidal activity[J]. Steroids, 2015, 102: 17-26.
[9]	Offner PJ, Moore EE, Biffl WL. Male gender is a risk factor for major infections after surgery[J]. Arch Surg, 1999, 134(9): 935-938, 938-940.
[10]	Kisat M, Villegas CV, Onguti S, et al. Predictors of sepsis in moderately severely injured patients: an analysis of the National Trauma Data Bank[J]. Surg Infect (Larchmt), 2013, 14(1): 62-68.
[11]	Vasudevan N, Pfaff DW. Non-genomic actions of estrogens and their interaction with genomic actions in the brain[J]. Front Neuroendocrinol, 2008, 29(2): 238-257.
[12]	Johnson BD, Zheng W, Korach KS, et al. Increased expression of the cardiac L-type calcium channel in estrogen receptor-deficient mice[J]. J Gen Physiol, 1997, 110(2): 135-140.
[13]	Yang X, Chen G, Papp R, et al. Oestrogen upregulates L-type Ca2+ channels via oestrogen-receptor- by a regional genomic mechanism in female rabbit hearts[J]. J Physiol, 2012, 590(3): 493-508.
[14]	Marni F, Wang Y, Morishima M, et al. 17 beta-estradiol modulates expression of low-voltage-activated Ca(V)3.2 T-type calcium channel via extracellularly regulated kinase pathway in cardiomyocytes[J]. Endocrinology, 2009, 150(2): 879-888.
[15]	朱颖, 严静. 钙平衡失调在脓毒症心肌损伤中的作用[J]. 心脑血管病防治, 2014, 14(2): 149-151.
[16]	Hobai IA, Edgecomb J, Labarge K, et al. Dysregulation of intracellular calcium transporters in animal models of sepsis-induced cardiomyopathy[J]. Shock, 2015, 43(1): 3-15.

内源性雌激素调控脓毒症小鼠心肌L型钙离子通道α1C亚基的表达

Endogenous estrogen regulates the expression of myocardial L-type calcium channel α1C subunit (CACNA1C) in septic mice

可视化

摘要/Abstract

引用本文

使用本文

参考文献 16

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	江剑宏, 段仁鹏, 李晓锋. 国人胆道三维重建解剖变异应用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 1-4.
[2]	郭红志, 王瑜, 张加琪, 梁海彬, 马梓玮, 冯伟, 吴忧, 司子燚. 血管铸型结合CT三维模型评估先兆子痫胎盘血管结构[J]. 中国临床解剖学杂志, 2024, 42(1): 5-10.
[3]	李佳伟, 张静, 李灿然, 兰文杰, 籍庆余, 郭志勇, 张云凤, 刘启, 陈清威, 李筱贺. 蒙古族人群股骨近端解剖参数X线测量[J]. 中国临床解剖学杂志, 2024, 42(1): 11-16.
[4]	李琨, 张燕, 郭冉, 郝咪咪, 吴轩宇, 徐艺芳, 王超群, 马文童, 张灵淇, 杨宏宇, 李志军, 张少杰, 王星. 儿童及青少年枕颈角与后枕颈角数字化测量及其临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 17-20.
[5]	李文, 杨思艺, 黄蕾, 卿霁雯, 蒋松涛, 张磊. 基于MRI探讨Lisfranc韧带的形态学特点及临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 21-25.
[6]	李凡凡, 徐阳, 王晓旭. 紫草素调节Nrf2/HO-1信号通路对实验性大鼠肉芽肿性小叶性乳腺炎的治疗作用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 26-32.
[7]	王兴航, 丁佳媛, 李放, 包翠芬, 阎丽菁. 人参皂苷Rg1通过抑制NLRP3炎症小体途径减轻氧糖剥夺/复供后小胶质细胞炎症反应[J]. 中国临床解剖学杂志, 2024, 42(1): 33-41.
[8]	程莹莹, 武建军, 蔡海燕, 焦旭文, 马江波, 丁银秀. 体外炎性诱导星形胶质细胞激活及功能分析[J]. 中国临床解剖学杂志, 2024, 42(1): 42-46.
[9]	祁志, 杨力, 贾秋叶, 陈浩伦, 段兆达, 吴春云. 依达拉奉经MAPKs通路对脂多糖激活的小胶质细胞Sirt3表达调控[J]. 中国临床解剖学杂志, 2024, 42(1): 47-53.
[10]	张晶晶, 王洪新. 黄芩苷通过PDGF/P38 MAPK信号通路对肺动脉高压大鼠的保护作用[J]. 中国临床解剖学杂志, 2024, 42(1): 54-58.
[11]	乐钦, 陈荣丽, 熊婧熙, 王婷怡, 蔡志恒, 易秋实, 曾心怡. 白芨多糖对大鼠跨区皮瓣choke区的影响[J]. 中国临床解剖学杂志, 2024, 42(1): 59-64.
[12]	李笑予, 张磊, 付磊, 李东波, 汪国友. 三步接骨法治疗Sanders Ⅱ型跟骨骨折的有限元研究[J]. 中国临床解剖学杂志, 2024, 42(1): 65-70.
[13]	吴研飞, 马剑雄, 卢斌, 王颖, 柏豪豪, 靳洪震, 马信龙. CT后处理技术重建CT值与终板抗压强度的相关性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 71-76.
[14]	庄万强, 唐毅, 骆勇刚, 张辉. 关节镜联合胫骨高位截骨术对髌骨位置及髌股关节功能影响的早中期回顾性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 77-82.
[15]	罗鹰, 窦伟誉, 吴小杭, 陈璟昆, 彭昌贵, 潘剑英 . 微创海马钢板内固定治疗跟骨骨折并发症的影响因素[J]. 中国临床解剖学杂志, 2024, 42(1): 83-88.