改变肌动蛋白聚合状态调控人成纤维细胞成骨分化的线粒体动力学

doi:10.13418/j.issn.1001-165x.2020.03.006

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (3): 263-269.doi: 10.13418/j.issn.1001-165x.2020.03.006

改变肌动蛋白聚合状态调控人成纤维细胞成骨分化的线粒体动力学

江鑫，　樊庭宇，　孙冰，　戴景兴，　欧阳钧，　钟世镇

南方医科大学人体解剖学教研室，广东省医学生物力学重点实验室，广州 510515

收稿日期:2020-04-10 出版日期:2020-05-25 发布日期:2020-06-02
通讯作者: 欧阳钧，教授，博士生导师，E-mail：jouyang@smu.edu.cn 钟世镇，院士，博士生导师，E-mail：zhszh@smu.edu.cn
作者简介:江鑫（1992-），女，湖南长沙人，硕士研究生，研究方向：组织工程与再生医学，E-mail：545767454@qq.com
基金资助:
国家重点研发计划资助项目（2017YFC1105000）；深圳市医疗卫生三名工程（SZSM201612019）

Actin polymerization affects osteogenic differentiation of human fibroblast by regulating mitochondrial dynamics

JIANG Xin, FAN Ting-yu, SUN Bing, DAI Jing-xing, OUYANG Jun, ZHONG Shi-zhen

Guangdong Provincial Key Laboratory of Medical Biomechanics, Department of Anatomy, Southern Medical University, Guangzhou 510515, China

Received:2020-04-10 Online:2020-05-25 Published:2020-06-02

摘要/Abstract

摘要： 目的　检测在人成纤维细胞成骨分化过程中肌动蛋白的改变及其介导的线粒体动力学改变。方法　诱导人成纤维细胞成骨分化，检测肌动蛋白和线粒体动力学的改变。在人成纤维细胞中打断（细胞松弛素D，Cyto D）或者促进（Jasplakinolide，JAS）肌动蛋白的聚合，于处理的第1、4、7 d分别进行免疫荧光和Western blot等实验检测细胞性能及线粒体动力学相关指标。结果　Cyto D减弱成骨诱导液效果，JAS增强成骨诱导液效果。JAS增强线粒体动力学效果比Cyto D更加显著。结论　肌动蛋白的聚合促进细胞成骨分化；肌动蛋白的聚合或解聚影响细胞成骨分化过程中线粒体与细胞骨架肌动蛋白的共定位比率。Cyto D和JAS引起的对细胞成骨分化的影响和线粒体动力学的改变，可为研究细胞线粒体以及成骨分化机制提供生物学信息。

关键词: 线粒体, 　成骨分化, 　细胞松弛素D, 　细胞骨架稳定剂

Abstract: Objective　To explore the affects of actin on osteogenic differentiation of human fibroblast by regulating mitochondrial dynamics.　Methods 　Cytochalasin D (Cyto D) and Jasplakinolide (JAS) were added to be the growth medium and osteogenic differentiation medium respectively, then the changes of cell characters and mitochondrial dynamics were detected by Immunofluorescence staining and western blot experiments in the treatment of 1 d, 4 d and 7 d.　Results　Cyto D reduced the effect of osteogenic induction media, but JAS increased the effect of it. JAS enhanced significantly the effect of mitochondrial dynamics in fibroblast than Cyto D.　Conclusions　Actin polymerization motivates the osteogenic differentiation; Actin polymerization and depolymerization affects the colocalization ratio between mitochondrial and actin in osteogenic differentiated cells. The results can provide important biological information for studying the mitochondrial and differentiation mechanism.

中图分类号:

Q245

江鑫, 樊庭宇, 孙冰, 戴景兴, 欧阳钧, 钟世镇. 改变肌动蛋白聚合状态调控人成纤维细胞成骨分化的线粒体动力学[J]. 中国临床解剖学杂志, 2020, 38(3): 263-269.

JIANG Xin, FAN Ting-yu, SUN Bing, DAI Jing-xing, OUYANG Jun, ZHONG Shi-zhen. Actin polymerization affects osteogenic differentiation of human fibroblast by regulating mitochondrial dynamics[J]. Chinese Journal of Clinical Anatomy, 2020, 38(3): 263-269.

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改变肌动蛋白聚合状态调控人成纤维细胞成骨分化的线粒体动力学

Actin polymerization affects osteogenic differentiation of human fibroblast by regulating mitochondrial dynamics

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