小鼠臂丛离断对脊髓运动神经元树突结构与形态退变的影响

doi:10.13418/j.issn.1001-165x.2022.3.07

摘要/Abstract

摘要： 目的研究臂丛离断后脊髓运动神经元树突退变与时间和损伤距离的相关性。方法在距离椎间孔3 mm或10 mm处处离断小鼠臂丛，术后7、14、28、56 d取材，采用MAP2免疫荧光染色和体视学分析、Golgi-Cox染色和Sholl分析观测颈膨大处脊髓前角运动神经元的树突结构和形态变化；术后28 d比较距离椎间孔3 mm和10 mm臂丛离断对脊髓运动神经元树突的影响。结果 MAP2免疫荧光显示臂丛离断导致脊髓前角内树突的密度和完整性随时间延长逐渐下降；Golgi-Cox染色和Sholl分析显示运动神经元最长树突、总树突长度、树突最大跨度、树突3级分支的数量均呈时间依赖性下降。与距离椎间孔10 mm处离断组相比，3 mm处离断引起的树突长度退变更为明显。结论脊髓运动神经元树突在周围神经损伤后会发生退变，随时间延长其退变程度加重，随损伤部位与脊髓的距离延长树突长度退变程度减轻。

关键词: 树突； , 运动神经元； , , 臂丛离断； , , 脊髓

Abstract: Objective To study the correlation of time course and distance of brachial plexus transection on the dendritic degeneration of spinal motoneurons. Methods The brachial plexus of mice was transected at 3 mm or 10 mm from the intervertebral foramen, the spinal cord segments of C6~C7 was collected at 7, 14, 28 and 56 days post injury (dpi) and was subjected to perform MAP2 immunofluorescence staining, morphometric analysis, Golgi-Cox staining and Sholl analysis to assess the dendritic structure changes of spinal motoneurons. The effects of brachial plexus transection 3 mm and 10 mm from the foramina on spinal motor neuron dendrite was compared at 28 days after surgery. Results The brachial plexus transection resulted in that the density and integrity of dendrite in the spinal ventral horn decreased gradually with time. Golgi-Cox staining and Sholl analysis showed the longest dendrite, total dendritic length, the maximum span of dendrite and the number of third-grade dendrite branches of each spinal motoneuron decreased with time dependence. Compared with the transection at 10 mm from the intervertebral foramen, the length of dendritic degeneration in the 3 mm group was more severe. Conclusions The dendrite degeneration occurs and get worse in spinal motoneurons after peripheral nerve injury with time dependence, while the degree dendrite degeneration reduces with the extension of the distance between injury site and spinal cord.

Key words: Dendrites; , , Motoneuron; , , Brachial plexus transection; , , Spinal cord

中图分类号:

R322.85

李咪, 许嘉玮, 邹颖, 郭家松. 小鼠臂丛离断对脊髓运动神经元树突结构与形态退变的影响[J]. 中国临床解剖学杂志, 2022, 40(3): 277-285.

Li Mi, Xu Jiawei, Zou Ying, Guo Jiasong. The effects of brachial plexus transection on the structure and morphology degeneration of spinal motoneuron dendrites in mice[J]. Chinese Journal of Clinical Anatomy, 2022, 40(3): 277-285.

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