GSK429286A促进大鼠皮瓣术后存活与感觉恢复的研究

doi:10.13418/j.issn.1001-165x.2024.6.11

中国临床解剖学杂志 ›› 2024, Vol. 42 ›› Issue (6): 673-679.doi: 10.13418/j.issn.1001-165x.2024.6.11

GSK429286A促进大鼠皮瓣术后存活与感觉恢复的研究

林加福¹，王建红²，方芳³，刘东红⁴*

1.福建卫生职业技术学院解剖教研室，福建福州 350101； 2.宁波大学附属第一医院小儿整形外科，浙江宁波 315000；
3；福建医科大学基础药学院，福建福州 350122； 4.山东第一医科大学第二附属医院烧伤整形美容科，山东泰安 271000

收稿日期:2024-04-18 出版日期:2024-11-25 发布日期:2024-12-16
通讯作者: 刘东红，主治医师，E-mail：39145958@qq.com
作者简介:林加福(1984-)，讲师，主要从事皮瓣的临床应用解剖，E-mail：249973948@qq.com
基金资助:
福建省中青年教师教育科研项目（JAT210792），福建省自然科学资金项目（2020J01625）

Augmentation of flap survival and sensory recovery in rats with GSK429286A

Lin Jiafu¹, Wang jianhong², Fang Fang³, Liu Donghong⁴*

1.Department of Human Anatomy, Fujian Health Vocational and Technical College, Fuzhou 350101，2. Department of Pediatric Plastic Surgery, The First Affiliated Hospital of Ningbo University，Ningbo 315000，Fujian province, China; 3. School of Basic Medicine, Fujian Medical University, Fuzhou 350122; 4. Department of Burn and Plastic Surgery, The Second Affiliated Hospital of Shandong First Medical University, Tai'an 271000, Shandong Province, China

Received:2024-04-18 Online:2024-11-25 Published:2024-12-16

摘要/Abstract

摘要： 目的探讨强ROCK抑制剂GSK429286A促进大鼠背部跨区皮瓣存活及神经再生的效果。方法取SD雄性大鼠32只，以髂腰穿支为蒂，设计切取背部大小为10 cm×3 cm跨区皮瓣。后将大鼠等分为对照组与GSK429286A组（按10 mg/kg腹腔给药），每组又分入7 d与30 d两个时间点。术后7 d，对皮瓣进行拍照，测量皮瓣坏死率。接着，利用激光多普勒称比成像仪对第二choke区的血流灌注进行测定。术后30 d，利用躯干皮肌反射对两组皮瓣的感觉恢复情况进行评估。术后7 d和30 d取choke二区组织，利用α-SMA与NF-200一抗分别对皮瓣内的血管和神经进行染色。体外研究部分，取15 d孕鼠DRG，分成对照组与GSK429286A（10 mmol/L）组。培养3 d后，利用Tuj1一抗对DRG轴突进行染色，后测量每个DRG轴突的长度。结果对照组与GSK429286A组皮瓣坏死率分别为(19±7）% 与（10±5）%，两者具有显著统计学差异（P=0.012）。对照组与GSK429286A组第二choke区的血管管径分别为(51±8) μm与（76±7）μm，两者具有显著统计学差异（P<0.001）。对照组与GSK429286A组第二choke区血流灌注分别为(115±11) PU与(163±12) PU，两者具有显著统计学差异（P<0.001）。术后30 d，GSK429286A组大鼠皮瓣内能观察到丰富的NF-200阳性的神经纤维，躯干皮肌反射为阳性，而对照组大鼠皮瓣内未能观察到NF-200阳性的神经组织，躯干皮肌反射为阴性。结论强ROCK抑制剂GSK429286A能够同时促进大鼠皮瓣术后存活与感觉恢复。

关键词: GSK429286A, , , ROCK抑制剂, , , 皮瓣坏死, , , 神经再生

Abstract: Objective o explore the efficacy of the strong ROCK inhibitor GSK429286A in promoting the survival and sensory recovery of extended flaps in rats. Methods 32 male SD rats were adopted and extended flaps measuring 10 cm×3 cm based on the iliolumbar perforator were harvested. The rats were then equally divided into a control group and a GSK429286A group (administered intraperitoneally at a dose of 10 mg/kg), and into 7 and 30 days after surgery. At day 7, the flaps were photographed for calculation of necrosis rates. Subsequently, laser Doppler contrast imaging was used to assess blood perfusion in the second choke zone. At day 30, the sensory recovery of the flaps in both groups was evaluated using the cutaneous trunci muscle reflex (CTMR). Tissue samples from the second choke zone were collected at day 7 and 30, and immunohistochemical staining with α-SMA and NF-200 antibodies was performed to visualize the vessels and nerves. For in vitro study, dorsal root ganglia (DRG) were obtained from 15-day pregnant rats and divided into the control and GSK429286A groups. After treatment with GSK429286A (10 mmol/L) for 3 days, Tuj1 antibody was used to stain the DRG axons, and the length of DRG axons was measured. The Independent sample t-test was used to compare the data between the two groups. Results The necrosis rates in the control and GSK429286A groups were (19±7) % and (11±5) %, respectively, showing a significant difference (P=0.032). The vascular diameter in the second choke zone of the control and GSK429286A groups were 81±8 um and 106±7 um, respectively, also demonstrating a significant difference (P<0.001). In accordance, blood perfusion in the second choke zone was (67±5) PU in the GSK429286A group, significantly higher than 45±11 PU in the control group (P=0.002). At day 30, abundant NF-200-positive nerve fibers could be observed within the flaps of the GSK429286A group, resulting in positive CTMR. In contrast, no NF-200-positive nerve fibers could be observed in the control group, resulting in negative CTMR. Conclusions The strong ROCK inhibitor GSK429286A can simultaneously promote the survival and sensory recovery of flaps in rats.

Key words: GSK429286A, , , ROCK inhibitor, , , Flap necrosis, , , Nerve regeneration

中图分类号:

R625.3

林加福, 王建红, 方芳, 刘东红. GSK429286A促进大鼠皮瓣术后存活与感觉恢复的研究[J]. 中国临床解剖学杂志, 2024, 42(6): 673-679.

Lin Jiafu, Wang jianhong, Fang Fang, Liu Donghong. Augmentation of flap survival and sensory recovery in rats with GSK429286A[J]. Chinese Journal of Clinical Anatomy, 2024, 42(6): 673-679.

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GSK429286A促进大鼠皮瓣术后存活与感觉恢复的研究

Augmentation of flap survival and sensory recovery in rats with GSK429286A

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