目的 针对实验动物显微外科技术培训需求，建立一种可重复操作的颈深部淋巴静脉吻合大鼠模型，以提升显微外科医师在颈深部解剖区域的淋巴管静脉吻合的操作能力。方法 选取20只4~5周龄的SPF级雄性SD大鼠（体重约100 g），腹腔注射戊巴比妥钠（45 mg/kg）麻醉后仰卧位固定。于鼻粘膜下注射稀释吲哚菁绿（ICG）0.05~0.10 mL（浓度：0.1 mg/mL），沿颈部中线作1.5 cm切口，结合术中荧光成像技术，暴露颈深淋巴管及颈外静脉。在放大25倍下行淋巴静脉端端吻合或端侧吻合。结果 颈深部备选淋巴管平均直径0.18 mm（0.1~0.4 mm），静脉管直径0.6~1.2 mm。完成20处吻合，即刻淋巴静脉再通率95%（19/20），失败1例。结论 该模型可模拟临床颈深部淋巴静脉吻合场景，同时具备解剖准备和操作重复性，为颈深淋巴静脉吻合训练提供了一种可操作动物模型。
Abstract
Objective To establish a rat model of deep neck lymphatic vein anastomosis with complex anatomy and repeatable operation in response to the training needs of experimental animal microsurgery techniques, in order to enhance the operational ability of microsurgery physicians in lymphatic vein anastomosis in the deep neck anatomical area. Methods Twenty 4-5 week old SPF grade male SD rats (weighing approximately 100 g) were selected and anesthetized with pentobarbital sodium (45 mg/kg) via intraperitoneal injection, and then fixed in a supine position. Diluted indocyanine green (ICG) 0.05-0.1 mL (concentration: 0.1 mg/mL) was injected under the nasal mucosa to visualize lymphatic vessels, make a 1.5 cm incision along the midline of the neck, and intraoperative fluorescence imaging technology was used to expose deep cervical lymphatic vessels and external jugular veins. Lymphatic vein end-to-end anastomosis or end-to-end anastomosis at a magnification of 25 times was performed. Results The average diameter of the candidate lymphatic vessels in the deep neck was 0.18 mm (0.1-0.4 mm), and the diameter of the venous vessels was (0.6-1.2) mm. 20 cases of anastomosis were completed, with an immediate lymphatic vein recanalization rate of 95% (19/20) and 1 failure. Conclusions This model can simulate the clinical scenario of deep neck lymphatic vein anastomosis, and has both anatomical preparation and operational repeatability, providing an operable animal model for training deep neck lymphatic vein anastomosis.
关键词
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颈深部淋巴静脉吻合 /
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动物模型 /
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显微外科 /
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技术培训
Key words
Deep cervical lymphaticovenous anastomosis /
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Animal model /
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Microsurgery /
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Skill training
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基金
浙江省卫生厅科技计划项目(2024KY022)
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