目的 评估骨髓间充质干细胞（BMSC）在卵巢移植放疗后中的保护作用。 方法 将40只雌性Wistar近交系大鼠随机分为对照组和观察组，每组各20只。另取10只大鼠分离BMSC并进行培养及鉴定。对照组大鼠接受卵巢移植+200 cGy射线照射+磷酸盐缓冲溶液，观察组大鼠接受卵巢移植+200 cGy射线照射+BMSC移植。移植1月后观察大鼠一般情况及不良反应并处死所有大鼠，采用放射免疫分析法测定大鼠血清雌二醇（E2）、黄体生成素（LH）和卵泡刺激素（FSH）水平。通过原位末端转移酶标记技术检测大鼠卵巢颗粒细胞凋亡情况。 结果 P3 BMSC免疫表型显示，表面标记CD44（+）和CD90（+）的高表达率分别为培养细胞的78.04%和75.17%，此外CD45（+）和CD34（+）表达率较低，仅为8.05%和10.40%。观察组经BMSC移植后，大鼠饮食、皮毛、行为和精神状态等一般状况较对照组恢复较快，体重出现明显增长（P<0.05），不良反应率较低（P=0.010）。此外，与对照组比较，观察组大鼠血清E2水平明显升高，LH、FSH水平明显降低（P<0.001）。同时，观察组大鼠卵巢颗粒细胞凋亡率也较对照组明显下降，差异有统计学意义（P<0.001）。 结论 BMSC可在卵巢移植放疗后发挥保护作用，促使血清E2水平升高，LH、FSH水平降低，进而抑制卵巢颗粒细胞凋亡。
Abstract
Objective To evaluate the protective effect of bone marrow mesenchymal stem cells (BMSC) on ovarian transplantation after radiotherapy. Methods 40 female Wistar inbred rats were randomly divided into control group and observation group, with 20 rats in each group. BMSC were isolated from 10 rats and cultured and identified. The rats in control group received ovarian transplantation +200 cGy radiation + phosphate buffer solution, and the rats in observation group received ovarian transplantation +200 cGy radiation +BMSC transplantation. The general condition and adverse reactions of rats were observed 1 month after transplantation, and all rats were killed. The serum levels of estradiol (E2), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured by radioimmunoassay. The apoptosis of rat ovarian granulosa cells was detected by in situ end transferase labeling technique. Results The immunophenotype of P3 BMSC showed that the high expression rates of surface markers CD44 (+) and CD90 (+) were 78.04% and 75.17% of cultured cells, respectively. In addition, the expression rates of CD45 (+) and CD34 (+) were relatively low, only 8.05% and 10.40%. After BMSC transplantation, the diet, fur, behavior and mental state of rats in the observation group recovered faster than that in the control group, the body weight increased significantly (P<0.05), and the adverse reaction rate was lower (P=0.028). In addition, compared with the control group, the serum E2 level of rats in the observation group was significantly increased, and the LH and FSH levels were significantly decreased (P<0.001). At the same time, the apoptosis rate of ovarian granulosa cells in the observation group was significantly lower than that in the control group, with statistical significance (P<0.001). Conclusions BMSC can play a protective role after radiotherapy for ovarian transplantation, increase the level of serum E2, decrease the level of LH and FSH, and inhibit the apoptosis of ovarian granulosa cells.
关键词
骨髓间充质干细胞 /
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卵巢移植 /
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放疗 /
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生殖内分泌激素 /
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卵巢颗粒细胞
Key words
Bone marrow mesenchymal stem cells /
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Ovarian transplantation /
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Radiotherapy /
Reproductive endocrine hormone /
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Ovarian granulosa cell
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基金
江西省卫生健康委科技计划（SKJP220227959）
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