不同年龄兔脑基底动脉缝隙连接蛋白Cx37 的表达及意义

中国临床解剖学杂志 ›› 2010, Vol. 28 ›› Issue (3): 321-.

不同年龄兔脑基底动脉缝隙连接蛋白Cx37 的表达及意义

林雪群¹，　祝高春¹，　万丽丹¹，　薛国勇¹，　唐迅²

1. 南昌大学医学院脑血管研究所解剖学教研室，南昌 330006； 2. 江西护理职业技术学院外科教研室，南昌 330029

收稿日期:2009-07-13 出版日期:2010-05-25 发布日期:2010-06-11
作者简介:林雪群（1956-）,男，江西省兴国县人，教授，博士，硕士生导师
基金资助:
江西省自然科学基金项目（2009GZY0134）；江西省教育厅科技资助项目(赣教技字[2007]80号)

Expression of gap junctional proteins Cx37 in cerebral basilar artery of different aged rabbits and its significance

LIN Xue-qun, ZHU Gao-chun，WAN Li-dan, et al.

Institute of cerebrovascular research, Department of Anatomy, Medical College of Nanchang University, Nanchang 330006, China

Received:2009-07-13 Online:2010-05-25 Published:2010-06-11

摘要/Abstract

摘要：

目的通过观察不同年龄兔脑血管缝隙连接蛋白（Cx37）的表达，探讨Cx37在调节脑血管舒缩活动方面的作用。方法应用逆转录-聚合酶链（RT-PCR）技术测定幼年、成年和老年兔脑基底动脉缝隙连接蛋白Cx 37mRNA的表达；应用Western blot法检测不同年龄兔脑基底动脉Cx37蛋白的表达。结果大白兔脑基底动脉血管组织中存在Cx37 的表达，并随年龄增加而增加，幼年兔表达较少，至成年兔达高峰，而老年兔逐渐减少；成年兔脑基底动脉Cx37 的表达较幼年兔明显增高，老年兔表达降低。结论不同年龄兔脑基底动脉血管组织缝隙连接蛋白的表达随年龄的增加而增加，尤其以成年兔明显，提示缝隙连接蛋白在调节成年兔脑血管运动和舒缩活动方面具有重要的作用；而老年兔脑血管缝隙连接蛋白表达减少，可能与老年性脑血管硬化有关。

关键词: 脑血管!缝隙连接蛋白!兔

Abstract:

Objective　To investigate the effects of gap junctional proteins Cx37 on regulating the vasoconstriction and vasodilation of cerebral vessels. Methods　Reverse transcription polymerase chain reaction(RT-PCR) and Western blotting were used to examine the expression of Cx37 in cerebral basilar artery of rabbits at different ages, respectively. Results Cx37 mRNA expression in basilar artery was positive from young rabbit, and the expression reached a peak at adult stage of animals, and then descended again at the old stage. Cx37 protein expression in basilar artery of rabbits was stronger at adult stage than that of young and old stages. Conclusions　The expression of cx37 in the basilar artery of rabbit increased gradually, until the adult stage of animal, which implicates that, Cx37 may play an important role in regulating the cerebral vasculomotor activity. However, the decreased expression level of the protein for older animals may relate to the development of cerebrovascular sclerosis.

Key words: Cerebral vessel!Gap junctional protein!Rabbit

中图分类号:

R331.37

林雪群, 祝高春, 万丽丹, 薛国勇, ??迅. 不同年龄兔脑基底动脉缝隙连接蛋白Cx37 的表达及意义[J]. 中国临床解剖学杂志, 2010, 28(3): 321-.

LIN Xue-Qun, CHU Gao-Chun, MO Li-Dan, XUE Guo-Yong, TANG Xun. Expression of gap junctional proteins Cx37 in cerebral basilar artery of different aged rabbits and its significance [J]. Chinese Journal Of Clinical Anatomy, 2010, 28(3): 321-.