1型糖尿病大鼠左心室HCN2和HCN4重构

中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (3): 321-324.

1型糖尿病大鼠左心室HCN2和HCN4重构

王庆志，　周　立，　王志勇，　郭志坤

新乡医学院人体解剖学教研室，河南新乡 453003

收稿日期:2010-12-25 出版日期:2011-05-25 发布日期:2011-05-21
通讯作者: 郭志坤(1956-)，男，河南商丘人，教授， Tel：(0373)3831862，E-mail：gzk@126.com E-mail:wqzmgl@126.com
作者简介:王庆志(1970-)，男，河南商城人，博士，副教授，主要从事心脏基础研究，Tel：(0373)3831899
基金资助:
河南省杰出青年科学基金（2005HANCET-15）

The expression of HCN2 and HCN4 in the left ventricle of type 1 diabetes rat

WANG Qing-zhi, ZHOU Li, WANG Zhi-yong, GUO Zhi-yong

Department of Anatomy, Xinxiang Medical College, Xinxiang 453003, China

Received:2010-12-25 Online:2011-05-25 Published:2011-05-21

摘要/Abstract

摘要：

目的　探讨1型糖尿病大鼠左心室肌HCN2和HCN4的表达时空变化。方法　60只大鼠随机分为正常对照组(N组, n=20)和1型糖尿病组(T1DM组, n=40)：腹腔注射链脲佐菌素制作大鼠1型糖尿病模型。90 d后，取左心室肌通过免疫荧光法检测HCN2、HCN4的定位；Western-blot、RT-PCR技术检测HCN2、HCN4在蛋白质及mRNA水平的变化。结果　免疫荧光结果显示HCN2在心室肌细胞膜上呈点状或短线状不连续表达；HCN4未见表达。Western-blot、RT-PCR结果显示T1DM组大鼠　HCN2、HCN4在蛋白及mRNA水平表达升高。结论　1型糖尿病大鼠左心室出现离子通道HCN2、HCN4表达升高，导致糖尿病心脏电重构，可能与糖尿病引起的室性心律失常有关。

关键词: HCN2, HCN4, 左心室, 1型糖尿病, 大鼠

Abstract:

Objective　To investigate the expression of HCN2 and HCN4 in the left ventricles of type 1 diabetes rat.　Methods　60 rats were randomly divided into control group (N, n=20) and diabetes group (T1DM, n=40). For T1DM group, streptozotocin was injected into peritoneal cavity of the animals to establish the model of type 1 diabetes. At the 90d after injecting, the expression of HCN2 and HCN4 were detected by immunofluorescent staining, Western-blot and RT-PCR respectively.　Results　Immuno-fluorescent staining presented the discontinuous punctiform or linear expression of HCN2 in cardiomyocytes membrane, but not for HCN4. However, Both HCN2 and HCN4 were up-regulated at protein(western-blot) and RNA (RT-PCR) levels compared to that of controls.　Conclusions　The over expression of HCN2 and HCN4 maybe participate in the electrical remodeling in DM rats and the developing of ventricular arrhythmia.

Key words: HCN2, HCN4, Left ventricle, T1DM, Rat

中图分类号:

R322.1

王庆志, 周　立, 王志勇, 郭志坤. 1型糖尿病大鼠左心室HCN2和HCN4重构[J]. 中国临床解剖学杂志, 2011, 29(3): 321-324.

WANG Qing-Zhi, ZHOU 　Li, WANG Zhi-Yong, GUO Zhi-Kun. The expression of HCN2 and HCN4 in the left ventricle of type 1 diabetes rat[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(3): 321-324.

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