炎性疼痛致大鼠海马CREB结合蛋白的表达变化
李花, 曹文宇, 徐杨, 罗雁威, 钟小林, 张建伟, 李昌琪, 罗学港, 戴茹萍
中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (5) : 546-549.
炎性疼痛致大鼠海马CREB结合蛋白的表达变化
Inflammatory pain induced CBP expression change in rat hippocampus
目的 探讨完全弗氏佐剂（Complete Freund’s Adjuvant，CFA）致大鼠炎性疼痛后海马区CREB结合蛋白（CREB Binding Protein，CBP)的表达变化及意义。 方法 大鼠随机分为正常组和实验组，实验组大鼠左侧足底皮下注射CFA和生理盐水混合溶剂100 μl，建立炎性疼痛模型，分为注射CFA后6 h、1 d、3 d、7 d和14 d组。采用Von Frey纤维观察不同时间点大鼠机械缩足阈值（Paw withdrawal
threshold, PWT）的变化；采用免疫组化法检测海马CBP的表达变化。 结果 足底注射CFA后1 h PWT即下降，并在6 h后达到最低值，3 d后逐渐上调，至14 d仍低于基础值。正常组大鼠海马各区均可见CBP大量表达。实验组大鼠两侧海马各区尤其是CA1区和齿状回（Dentate Gyrus，DG）区域CBP的表达随时间点变化呈现出先降低后升高再降低的双相表达趋势，即注射CFA后6 h、1 d，CBP表达下降，至注射CFA后3 d，CBP表达明显上调；7 d时CBP表达再次出现下调，持续至14 d。 结论 CFA能诱导大鼠产生为期2周以上的炎性痛病程；炎性疼痛时海马区CBP的表达呈现出双相性表达趋势，提示海马区的基因表达变化在炎性疼痛的产生和持续过程中起着重要的作用。
Objective To explore expression changes of CBP（CREB Binding Protein) in rat hippocampus followed by Complete Freund's Adjuvant(CFA) induced inflammatory pain. Methods Rats were received plantar subcutaneous injection of CFA and saline mixed solvent 100ul, for establishing inflammatory pain model. Model rats were randomly divided into normal group, CFA 6h, 1d, 3d, 7d and 14d groups. The change of paw withdrawal threshold (PWT) was observed by Von Frey fibers in rats at different time points; the expression of CBP was detected using immunohistochemistry. Results PWT decreased quickly at 1h after CFA injection, and to the lowest value at 6h. PWT gradually rose at 3d and was still lower than baseline at 14d. A number of CBP positive products can be seen in hippocampus. CBP was significantly decreased at 6h and 1d after CFA injection; and increased at 3d, then decreased again from 7d to 14d after CFA injection，especially in CA1 and DG. Conclusions CFA can induce inflammatory pain about 2 weeks. CBP in hippocampus shows biphasic expression following inflammatory pain，which indicate gene transcription change in hippocampus plays an important role in the induction and maintenance of persistent pain.
Inflammatory pain / Hippocampus / CBP / Gene transcription / Rat
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