复方丹参对严重烫伤大鼠血脑屏障的保护作用
The protection of salvia miltiorrhiza compostita to blood brain barrier on severe burn injuried rats
目的 观察复方丹参注射液对严重烫伤大鼠血脑屏障通透性及脑内ZO-1mRNA影响，探讨复方丹参对严重烫伤大鼠血脑屏障损伤的保护作用。 方法 将大鼠随机分成假烫伤组、烫伤组、复方丹参治疗组。紫外分光光度计检测各组大鼠脑组织内伊文氏蓝的含量，RT-PCR检测严重烫伤大鼠脑内ZO-1 mRNA表达变化。 结果 大鼠严重烫伤后3～48 h脑内ZO-1mRNA表达下降。脑内伊文氏蓝（EB）含量从烫伤后3h开始升高，在6～12 h显著升高。用复方丹参治疗后, 严重烫伤大鼠脑内EB含量降低，以3、6 h下降最明显（P<0.010）；脑内ZO-1mRNA表达升高，以烫伤3 h治疗组最为明显。 结论 复方丹参注射液可防止严重烫伤大鼠脑内ZO-1mRNA的下降，减轻严重烫伤对大鼠血脑屏障的损害。
Objective To explore the effects of salvia miltiorrhiza compositita on blood-brain barrier permeability and ZO-1 expression in burn injuried rats. Methods In this research, healthy Sprague-Dawley rats were separated into three groups as follows: sham scald group, scald group, scald and salvia miltiorrhiza group. Changes in BBB permeability were determined by detected Evans blue (EB) content in rats' brain with chemical method. Furthermore, in order to explore the molecular mechanism of BBB disruption in rats' brain after severe burn, the expression levels of ZO-1 mRNA was analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results Expression of brain ZO-1mRNA significantly decreased during 3 to 48h after burn. The contents of Evan's bluedye (EB) in brain significantly improved at the 3h and reached the highest level during 6 to 24h after burn injury. After treating with salvia miltiorrhiza, EB content in the brain of burned rats was corrected obviously, especially at the time points of 3, 6h after burn injury. As well, ZO-1mRNA in brain increased after drug treating, and reached the highest level at 3h after burn injury. Conclusions Salvia miltiorrhiza will prevent the decrease of ZO-1 level in brain after burn injury, which is helpful for alleviating the burn damage on blood-brain barrier.
Burn injury / Blood-brain barrier / Zonula occludens / Salvia miltiorrhiza compostita(SMC)
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