血管生成素对糖尿病大鼠股骨头血管新生及渗漏的影响

中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (6): 681-685.

血管生成素对糖尿病大鼠股骨头血管新生及渗漏的影响

陈海英¹，　王秀国¹，　陈少强²，　刘文革³，　唐军民⁴

1.莆田学院医学院基础医学部，福建莆田 351100；　2.福建医科大学人体解剖学与组织胚胎学系，福州350003；
3.福建医科大学附属协和医院骨科，福州 350003； 4.北京大学医学部人体解剖与组织胚胎学系，北京 100191

收稿日期:2011-09-16 出版日期:2011-11-25 发布日期:2011-12-12
作者简介:陈海英（1968-），女，福建省莆田市人，硕士，副教授，主要从事组织退行性变方面的研究,Tel：(0594)2390302
基金资助:
福建教育厅科研基金资助项目（JB08222）

The effects of Ang-1 on the angiogenesis and vascular leakage of diabetic rats' femoral head

CHEN Hai-ying¹, WANG Xiu-guo¹, CHEN Shao-qiang ², LIU Wen-ge³ , TANG Jun-min⁴

1.Department of Basic Medicine, Medical college of Putian University, Putian 351100 ,China； 2.Department of Anatomy and Histology-Embryology, Fujian Medical University, Fuzhou 350004, China； 3. Department of Orthopedics, Union Hospital of Fujian Medical University, Fuzhou 350001, China； 4. Department of Anatomy and Histology -Embryology, Peking University Health Science Center, Beijing 100191, China

Received:2011-09-16 Online:2011-11-25 Published:2011-12-12

摘要/Abstract

摘要：

目的　探讨血管生成素1(angi.Poietin-1，Ang-1)对糖尿病大鼠股骨头微血管新生及渗漏的影响。方法　建立速发型链脲佐菌素（streptozotocin,STZ）糖尿病大鼠模型，随机分为正常5周组　(CON1)、10周组(CON2)及15周组(CON3)，糖尿病5周组（DM1）、10周组（DM2）及15周组（DM3），每组　10只。墨汁灌注观测股骨头微血管密度；摘取模型动物股骨头组织，免疫组化分析凝血因子Ⅷ（FⅧ）表达；原位杂交分析血管内皮生长因子（VEGFmRNA）表达强度；RT-PCR分析Ang-1的mRNA表达。结果　糖尿病大鼠股骨头随病程发展，Ang-1、FⅧ因子表达上升，与正常组相比有显著性差异（P<0.01）。　VEGFmRNA表达量均高于正常组（P<0.01）；微血管密度加大，显示血管增生、渗漏。结论　糖尿病股骨头　Ang-1与VEGFmRNA相互协同或拮抗分别促进微血管增生、抗血管渗漏。表达于血管内皮细胞的FⅧ及VEGFmRNA与微血管密度（MVD）变化存在正相关。

关键词: 糖尿病, 血管生成素1, 微血管, 血管内皮生长因子, FⅧ因子, 墨汁灌注, 原位杂交

Abstract:

Objective　To probe into the effects of Ang-1 on the angiogenesis and microvascular leakage of diabetic rats' femoral head.　Methods　By establishing models of the rats with STZ diabetes, and randomly dividing them into the normal groups (CON1, CON2 and CON3) and the diabetic groups (DM1, DM2 and DM3) with 10 rats each group, the microvascular density through infusion with ink, the expression of blood coagulation factorⅧ with immunohistochemistry, and the expression of the mRNA of both VEGF by hybridization in situ and Ang-1 by RT-PCR, were performed.　Results　The diabetic rats'femoral head varied in the course of illness. Their expression of Ang-1, blood coagulation factorⅧ, and VEGF mRNA significantly rose, as compared with those of rats in the normal groups(P<0.01). The microvascular density enlarged with vascular proliferation and leakage.　Conclusions　The interaction and antagonism between Ang-1 and VEGF mRNA of diabetic rats' femoral head can promote microvascular proliferation and resist vascular leakage. The mRNA expression of both coagulation factorⅧ and VEGF in vascular endothelial cells have a positive correlation with the changes of their microvascular density.

Key words: Diabetes, Ang-1, Micrangium, Vascular endothelial growth factor, F-Ⅷ, Infusion with ink, Hybridization in Situ

中图分类号:

R587.2

陈海英, 王秀国, 陈少强, 刘文革, 唐军民. 血管生成素对糖尿病大鼠股骨头血管新生及渗漏的影响[J]. 中国临床解剖学杂志, 2011, 29(6): 681-685.

CHEN Hai-Yang, WANG Xiu-Guo, CHEN Shao-Jiang, LIU Wen-Ge, TANG Jun-Min. The effects of Ang-1 on the angiogenesis and vascular leakage of diabetic rats' femoral head[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(6): 681-685.

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