可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的生物相容性实验研究
陈彦, 于博, 李松建, 陈仲, 刘成龙
中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (6) : 686-689.
可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的生物相容性实验研究
The biocompatibility of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold
目的 评价可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的组织相容性，以及探讨材料负载骨髓基质干细胞进行骨修复的可行性。 方法 壳聚糖/纳米羟基磷灰石/胶原材料与BrdU标记的rBMSc混合，注射入大鼠皮下，于皮下植入后24 h，14 d和28 d分别断颈处死大鼠，进行大体观察、HE、MASSON染色、免疫组化染色。 结果 壳聚糖/纳米羟基磷灰石/胶原材料在室温呈液态，经1 ml注射器26号针头注射入大鼠皮下后，材料在体内可以迅速原位成形成凝胶状态，并在原位保持形状，BrdU标记的rBMSc弥散于材料中，28 d的免疫组化结果显示细胞存活良好。 结论 壳聚糖/纳米羟基磷灰石/胶原复合材料具有良好的组织相容性，是一种良好的负载干细胞的骨组织工程支架。
Objective To evaluate the biocompatibility of an injectable chitosan (CS) /nano-hydroxyapatite(HA)/collagen (Col) scaffold. Methods rBMSCs was mixed with CS/HA/Col solution and subcutaneously injected into Wistar rats. After 24h, 14d and 28d, the animals were sacrificed and subcutaneous tissues at injecting site were removed. HE, MASSON and immunohistochemical stainings were performed for evaluating the features if scaffolds. Results The liquid CS/HA/Col (room temperature) would form the gel after injecting into the subcutaneous tissue. The mixed rBMSCs survived well in the CS/HA/Col gel at even 28 days in vivo. The CS/HA/Col/rBMSCs gel induced less inflammatory reaction in the host tissue compared to that of simple CS/HA/Col gel. Conclusions Our results suggest that the CS/HA/Col system can be used to load rBMSCs in vitro homogeneously. The CS/HA/Col system can be used in vivo with the minimally invasive manner and feasible biocompatible environment.
[1] Zhang W, Liao SS, Cui FZ. Hierarchical self-assembly of nano-fibrils in mineralized collagen
[J]. Chemistry of Materials,2003, 15(16):3221–3226.
[2] Huang Z, Feng QL, Yu B, et al. Biomimetic properties of an injectable chitosan/nano -hydroxyapatite/collagen composite
[J].Materials Science and Engineering,2011,31(3):683–687.
[3]Porter JR, Ruckh TT, Popat KC. Bone tissue engineering: a review in bone biomimetics and drug delivery strategies
[J]. Biotechnol Prog, 2009, 25(6): 1539-1560.
[4]Di Martino A, Sittinger M, Risbud MV. Chitosan：a versatile biopolymer for orthopaedic tissue-engineering
[J]. Biomaterials,2005, 26(30):5983-5990.
[5]Hoemann CD, Sun J, Legare A, et al. Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle
[J]. Osteoarthritis Cartilage, 2005, 13(4): 318-329.
[6] Ma GP, Yang DZ, Li QZ, et al. Injectable hydrogels based on chitosan derivative/polyethylene glycol dimethacrylate/N, N-dimethyla crylamide as bone tissue engineering matrix
[J]. Carbohydrate Polymers, 2009,79(3):620–627.
[7] Muzzarelli RAA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone
[J]. Carbohydrate Polymers, 2009, 76(2):167–182.
[8]Bartholomew A, Sturgeon C, Siatskas M, et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo
[J]. Experimental Hematology, 2002,30(1): 42–48.
[9]Di Nicola M, Carlo-Stella C, Magni M, et al. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellularor nonspecific mitogenic stimuli
[J]. Blood, 2002,99(10):3838–3843.
[10]Djouad F, Plence P, Bony C, et al. Immunosuppressiveeffect of mesenchymal stem cells favors tumor growth in allogeneic animals
[J]. Blood, 2003,102(10):3837-3844.
[11]Gratzner HG. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine:A new reagent for detection of DNA replication
[J]. Science,1982, 218(4571): 474-475.
[12]Sasaki T, Kitagawa K, Omura-Matsuoka E, et al. The phosphodiesterase inhibitor rolipram promotesSurvival of newborn hippocampal neurons after ischemia
[J]. Stroke, 2007, 38(5): 1597-1605.
[13]Zhao LR, Seema S, Duan WM, et al. Brain repair by hematopoietic growth factors in a rat model of stroke
[J]. Stroke, 2007, 38(9): 2584-2591.
广东省自然科学基金（10451051501004727），国家自然科学基金（81101348）
京ICP备08002354号-3/
| 〈 |
|
〉 |
