低频电磁场对BDNF基因修饰的BMSCs增殖影响的实验研究

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (1): 74-78.

低频电磁场对BDNF基因修饰的BMSCs增殖影响的实验研究

邱家琦¹，　张鸣生²，　李新平²，　白文芳²，　吴　博²，　白利明²，　徐武华¹

1.广州市红十字会医院，暨南大学医学院第四附属医院，广州 510220；
2.广东省人民医院，广东省医学科学院，广东省老年医学研究所，广州 510080

收稿日期:2011-08-07 出版日期:2012-01-25 发布日期:2012-02-03
通讯作者: 张鸣生，主任医师，Tel：020-83827812-70362，E-mail：mszrch@163.com E-mail:karke@163.com
作者简介:邱家琦(1982-)，男，广东化州人，汕头大学医学院研究生, 研究方向：神经康复学，E-mail：karke@163.com
基金资助:
广东省自然科学基金项目（项目编号9151008901000199）

The effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by BDNF gene

QIU Jia-qi¹, ZHANG Ming-sheng², LI Xin-ping², BAI Wen-fang², WU Bo², BAI Li-ming², XU Wu-hua¹

1. Red Cross Hospital, the Fourth Affiliated Hospital of Medical College, Ji'nan University, Guangzhou, 510220, China; 2. Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangdong Provincial Institute of Geriatric Medicine, Guangzhou, 510080, China

Received:2011-08-07 Online:2012-01-25 Published:2012-02-03

摘要/Abstract

摘要：

目的　探讨低频电磁场（LFEMF）对脑源性神经营养因子（BDNF）基因修饰的骨髓间充质干细胞（BMSCs）增殖的影响。方法　通过同源重组的方法，用pAd-easy I腺病毒包装系统在体外构建含BDNF基因的重组腺病毒表达载体(rADBDNF)；体外分离纯化SD大鼠来源的BMSCs，rADBDNF感染后，用LFEMF（50 Hz、5 mT）进行干预，连续干预3 d，并设对照组、BDNF基因修饰组、LFEMF组。在倒置相差显微镜下，对经台盼蓝染色的各组细胞进行计数；四甲基偶氮唑蓝（MTT）法对各组细胞的增殖效率进行检测；流式细胞仪分析细胞周期变化。结果　LFEMF作用于BDNF基因修饰后的BMSCs，细胞增殖速率与对照组、BDNF基因修饰组比较，差异有统计学意义（P<0.05），与LFEMF组相比，差异无统计学意义（P>0.05），G2/M期细胞数量明显增多。结论　LFEMF干预可促进BDNF基因修饰后BMSCs增殖。

关键词: 骨髓间充质干细胞, 细胞增殖, 脑源性神经营养因子, 低频电磁场

Abstract:

Objective　 To explore the effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by Brain-derived neurotrophic factor(BDNF) gene. 　Methods　The replication-deficient recombinant adenoviruses containing BDNF gene（rADBDNF）was structured by the pAd-easy I adenovirus packaging systems in vitro. Rat BMSCs were isolated from rat femoral and tibial bone marrow, cultured and identified. BMSCs infected with rADBDNF were exposed to low frequency electromagnetic fields. The proliferation capacity of BMSCs was analyzed using the trypan blue staining assay and MTT assay, and cell cycles of BMSCs was evaluated by flow cytometry.　Results　BMSCs modified by rADBDNF had no significant change on cell proliferation compared with that of untreated cells (P>0.05). However, electromagnetic field exposed MSCs(LFEMF group) had a significant proliferative improvement compared with the control(P<0.05). The ratio of G2/M cell of the transgenic and LFEMF group was also increased.　Conclusions　LFEMF could enhance the cell proliferation of rat BMSCs modified by BDNF gene.

Key words: Bone mesenchymal stem cells(BMSCs), Cell proliferation, Brain-derived neurotrophic factor(BDNF), Low frequency electromagnetic fields(LFEMF)

中图分类号:

R318.03

邱家琦, 张鸣生, 李新平, 白文芳, 吴博, 白利明, 徐武华. 低频电磁场对BDNF基因修饰的BMSCs增殖影响的实验研究[J]. 中国临床解剖学杂志, 2012, 30(1): 74-78.

QIU Jia-Qi, ZHANG Ming-Sheng, LI Xin-Beng, BAI Wen-Fang, TUN Bo, BAI Li-Meng, XU Wu-Hua. The effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by BDNF gene[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(1): 74-78.

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