损伤骨骼肌组织内CD8+ T细胞的功能特性

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (3): 307-310.

损伤骨骼肌组织内CD8⁺ T细胞的功能特性

冯利强^1,2，　曾慧君¹，　刘幸卉¹，　陈荣¹，　黄维一¹，　廖华¹

1. 南方医科大学解剖教研室，广东省组织构建与检测重点实验室，广州 510515；
2. 宁夏医科大学解剖教研室，宁夏银川 750004

收稿日期:2012-03-23 出版日期:2012-05-25 发布日期:2012-06-06
通讯作者: 廖华，教授，硕士生导师,Tel:62789092，E-mail: hua-liao@ 163.com
作者简介:共同第一作者：冯利强（1977-），男，讲师，硕士研究生；曾慧君（1988-），男，硕士研究生,研究方向：骨骼肌损伤相关研究
基金资助:
国家自然科学基金(81171724),(30771045)

Acute myoinjury induce the intramuscular infiltration of CD8+ CTL cells

FENG Li-qiang^1,2, ZENG Hui-jun¹, LIU Xin-hui ¹, CHEN Rong¹, HUANG Wei-yi¹, LIAO Hua¹

1. Department of Anatomy, The Key Lab of Construction and Detection of Guang Dong Province, Guangzhou, Southern Medical University, Guang Zhou, 510515 China; 2. Department of Anatomy，Ningxia Medical University, YingChuan, 750004 China

Received:2012-03-23 Online:2012-05-25 Published:2012-06-06

摘要/Abstract

摘要：

目的　分析骨骼肌损伤后CD8+ T细胞的渗出及其功能特性。方法　机械挤压法制备小鼠胫骨前肌（TA）损伤模型。免疫组化、免疫荧光检测损伤肌组织内CD8+ T细胞的渗出及其可能的CTL功能。结果　HE染色证实机械挤压引发显著的TA肌纤维坏死、退变和再生。损伤肌组织内出现广泛的炎性渗出，损伤后7d（再生初期）可见较多的CD8+ T细胞，少量CD8+ T细胞共表达Perforin以及　IFN-γ。结论　与慢性肌炎的免疫反应相似，急性损伤同样会引发CD8a+ T细胞的渗出、活化并具备　CTL功能。但这种活化的CD8+ CTL细胞仅短暂出现于肌组织的再生初期。

关键词: T细胞；　CD8；　骨骼肌损伤；　炎性反应

Abstract:

Objective　To investigate the intramuscular infiltration of CD8+ cytotoxic T-cells （CTL cells） after crush myoinjury.　Methods　Tibialis antierior muscle(TA) crush was performed in C57BL/6 mice. HE staining was used to identify inflammatory reaction，degeneration and regeneration of crushed TA muscle. Immunofluorecence was used to detect pro- and anti- inflammatory cytokines, and the intramuscular infiltration of CTLs in injuried muscle.　Results　Crush induced significant myofiber necrosis, degeneration and regeneration. Conspicuous mononuclear cell infiltrates were detected at both d4 and d7 after crush injury. At d7 post-injury CTLs expressing perforin or IFN-γ were conspicuously detected in crushed muscle where CD8+ T-cells were found in close contact with regenerating myofibers.　Conclusions　After crush injury, CD8+ T cells infiltrate the injured muscle at regenerating period, and are transiently activated as CTL cells.

Key words: T cell, CD8, Myoinjury, Nflammatory reaction

中图分类号:

R392.3

冯利强, 曾慧君, 刘幸卉, 陈荣, 黄维一, 廖华. 损伤骨骼肌组织内CD8⁺ T细胞的功能特性[J]. 中国临床解剖学杂志, 2012, 30(3): 307-310.

FENG Li-Jiang, CENG Hui-Jun, LIU Nie-Hui, CHEN Rong, HUANG Wei-Yi, LIAO Hua. Acute myoinjury induce the intramuscular infiltration of CD8+ CTL cells[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(3): 307-310.