维甲酸诱导脊柱裂胎鼠脊髓组织中Caspase-3表达情况

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (3): 311-314.

维甲酸诱导脊柱裂胎鼠脊髓组织中Caspase-3表达情况

马英桓^1，2，　袁正伟²

1.中国人民解放军第四六三医院，沈阳 110042; 2.中国医科大学附属盛京医院，沈阳 110004

收稿日期:2011-09-29 出版日期:2012-05-25 发布日期:2012-06-06
通讯作者: 袁正伟，教授, 博士生导师，E-mail：yuanzw@hotmail.com E-mail:myh463@yahoo.com.cn
作者简介:马英桓(1976-），男，回族，吉林省四平市人，硕士研究生，主要从事常见小儿先天畸形发生机制、早期诊断和治疗方面研究, Tel：024-28844247
基金资助:
国家自然科学基金（30872705，81070538）

Caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat

MA Ying-huan ^1,2 , YUAN Zheng-wei ²

1.Department of Cancer, No. 463 Hospital of the China People's Liberation Army, Shenyang 110042, China 2. The Major Laboratory of Health Ministry for Congenital Malformation Research, Shengjing Hospital of China Medical University, Shenyang 110004, China

Received:2011-09-29 Online:2012-05-25 Published:2012-06-06

摘要/Abstract

摘要：

目的　本文旨在探讨维甲酸诱导脊柱裂胎鼠脊髓组织Caspase-3表达情况。方法　选取孕10 d Wistar大鼠，实验组用溶有维甲酸（40mg/ml）的橄榄油，以135 mg/kg经胃管注入给药制作脊柱裂畸形大鼠模型；对照组选取孕10 d Wistar大鼠给等量橄榄油。将实验组及对照组按照孕12、15、17和20 d分为4组。应用免疫组织化学方法比较分析Caspase-3在对照组、畸形组胎鼠脊髓组织细胞中的分布和表达情况。结果　脊柱裂大鼠脊髓神经组织中Caspase-3在15 d开始增多，一直持续到20 d胚胎大鼠。其增高情况明显高于同一时间点对照组大鼠。胚胎15、17和20 d显性脊柱裂畸形鼠脊髓组织Caspase-3阳性细胞数多于对照组，荧光强度高于对照组。结论　维甲酸诱导的脊柱裂胎鼠Caspase-3表达明显高于正常发育胎鼠。

关键词: 神经管畸形, 脊髓组织, 细胞凋亡, Caspase-3

Abstract:

Objective To explore caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat.　Methods　Pregnant Wister rats with 10 days were used. Retinoic acid dissolved in olive oil (40mg /ml) were stomach fed for preparing the rat model of spina bifida malformations 135mg / kg). Control group only received olive oil. The animals were divided into 4 groups: pregnancy of 12 days, 15 days, 17 days and 20 days. Immunohistochemical method was used to detect and compare caspase-3 expression in different groups.　Results　The expression of caspase-3 increased at the day 15 after pregnancy, and maintained until day 20 in the spinal tissue of modeled fetal rat, which presented significant difference compared to that of control groups at the same pregnant time. At day 15, day 17 and day 20 of pregnancy, the number of caspase-3 positive cells was more in model animals than the control.　Conclusions　Retinoic acid induced spina bifida fetal rat demonstrates the increased caspase-3 expression in spinal tissue of fetal rats.

Key words: Neural tube defects, Spinal tissue, Apoptosis, Cspase-3

中图分类号:

R722.6

马英桓, 袁正伟. 维甲酸诱导脊柱裂胎鼠脊髓组织中Caspase-3表达情况[J]. 中国临床解剖学杂志, 2012, 30(3): 311-314.

MA Yang-Huan, YUAN Zheng-Wei. Caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(3): 311-314.

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