新型聚乳酸与纳米羟基磷灰石复合人工骨的性能测试研究

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (3): 324-328.

新型聚乳酸与纳米羟基磷灰石复合人工骨的性能测试研究

黄江鸿^1,3，　王大平^2,3，　刘建全^2,3，　马经野³

1.广州医学院，广州 510182；　2.深圳市第二人民医院创伤骨科，深圳 518035；
3.深圳市组织工程重点实验室，深圳 518035

收稿日期:2011-09-03 出版日期:2012-05-25 发布日期:2012-06-06
通讯作者: 王大平，教授，硕士生导师，E-mail:Dapingwang@　medmail.com E-mail:huangjianghong88@sohu.com
作者简介:黄江鸿（1982-），男，广州医学院在职硕士研究生，研究方向：骨组织工程、骨生物材料
基金资助:
广东省科技计划项目（83110）

The study of a new artificial bone consisted with polylactic acid and nano-hydroxyapatite

HUANG Jiang-Hong ^1,3，WANG Da-Ping ^2,3，LIU Jian-Qua ^2,3，MA Jing-Ye³

1.Guangzhou Medical College, Guangdong 510182, China; 2. Orthopaedics and Traumatology, Shenzhen Second People's Hospital,Guangdong 518035，China; 3. Shenzhen Tissue Engineering Key Laboratory, Guangdong 518035, China

Received:2011-09-03 Online:2012-05-25 Published:2012-06-06

摘要/Abstract

摘要：

目的　通过原位聚合法制备新型的聚乳酸与纳米羟基磷灰石复合材料，找到两者复合的最佳配比，从而得到理想的人工骨移植材料。方法　采用原位聚合法按一定的配比（纳米羟基磷灰石质量分数分别为0，10%，20%，30%，40%)聚乳酸与纳米羟基磷灰石复合人工骨，对这类新型的人工骨进行性能测试，通过抗弯，抗压，弹性模量，电镜扫描，体外降解实验，观测该人工骨的力学性能、微观结构、纳米羟基磷灰石在聚乳酸中的分散情况以及复合材料的降解性能。结果　（1）力学测试显示：随着n-HA含量的增加，复合材料的拉伸强度逐渐减少。复合材料的弯曲强度在n-HA微粒的质量分数为20%时弯曲强度出现峰值(156.8 MPa)。复合材料的弯曲模量随着n-HA微粒质量分数的增加而增大。（2）SEM扫描显示：纯PDLLA材料断裂表面较平整；在n-HA含量为10%时出现大量的韧窝, 明显的粗糙断裂面；在n-HA含量为20%断裂表面凹凸不平，形成大量的韧窝；在n-HA含量为30%以上时断口又变得越来越平整，尚有许多小的韧窝。（3）体外降解实验显示：随着降解时间的延长，降解液的pH值均逐渐降低，复合材料的力学性能也逐渐的产生一定的衰减。结论　当n-HA含量为20%时，该人工骨复合材料有着更好的力学性能和降解性能，筛选出该种新型人工骨的最佳配比，制备出性能良好的PDLLA/n-HA复合人工骨材料。

关键词: 聚乳酸, 纳米羟基磷灰石, 人工骨

Abstract:

Objective　To prepare a new artificial bone material by in situ polymerization of polylactic acid and nano-hydroxyapatite. Methods According to a specified ratio (the mass fraction of nano-hydroxyapatite respectively was 0, 10%, 20%, 30%, and 40%), polylactic acid and nano-hydroxyapatite were composed by in situ polymerization, followed by the testing of material parameters, including mechanical, ultrastructural, dispersional features in vitro. The degradation rate of composite material was detected. Results With the increase of n-HA content, the tensile strength of composite material decreased, but the elastic modulus increased. 20% of nano-hydroxyapatite content, made the best bending strength of composite material (156.8 MPa). SEM scan showed that, the fracture surface of pure PDLLA was relatively smooth; 10% of nano-hydroxyapatite content induced obvious dimples on the fracture surface. 20% nano-hydroxyapatite content induced uneven fracture surface, with a large number of dimples. However, 30% of nano-hydroxyapatite content made the fracture surface more flat. PH values of degradation liquid gradually reduced, as well the mechanical strength, following the extension of degradation. Conclusions Artificial bone material with 20% nano-hydroxyapatite content is ideal for its better mechanical properties and degradation properties.

Key words: Polylactic acid, Nano-hydroxyapatite, Artificial bone

中图分类号:

R318.08

黄江鸿, 王大平, 刘建全, 马经野. 新型聚乳酸与纳米羟基磷灰石复合人工骨的性能测试研究[J]. 中国临床解剖学杂志, 2012, 30(3): 324-328.

HUANG Jiang-Hong, WANG Da-Beng, LIU Jian-Quan, MA Jing-Shu. The study of a new artificial bone consisted with polylactic acid and nano-hydroxyapatite[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(3): 324-328.

参考文献

[1] Jayasuriya AC, Shah C, Ebraheim NA, et al. Acceleration of biomimetic mineralization to apply in bone regeneration
[J]. Biomed Mater, 2008,3(1):015003.

[2] Ren J，Zhao P，Ren T，et al. Poly (D,L-lactide)/nano-hydroxyapatite composite scaffolds for bone tissue engineering and biocompatibility evaluation
[J]. J Mater Sci Mater Med, 2008,19(3):1075–1082.

[3] Neuendorf RE, Saiz E, Tomsia AP, et al. Adhesion between biodegradable polymers and hydroxyaptite: relevance to synthetic bone-1ike materials and tissue engineering scaffolds
[J]. Acta Biomater, 2008, 4(5):1288-1296.

[4] Shen L， Yang H， Ying J, et al. Preparation and mechanical properties of carbon fiber reinforced hydroxyapatite/polylactide biocomposites
[J]. J Mater Sci Mater Med,2009, 20(11):2259-2265.

[5] 孙雪, 奚廷斐. 生物材料和再生医学的进展
[J].中国修复重建外科杂志, 2006,20(2):189-193.

[6] 顾汉卿. 生物医学材料的现状及发展(一)
[J].中国医疗器械信息, 2001,7(1):45-48.

[7] Holland SJ, Tighe BJ, Gould PL. Polymers for biodegradable medical devices. I: The potential of polyesters as controlled macromolecular release systems
[J]. Journal of Controlled Release, 1986, 4(3):155-180.

[8] Degee P, Dubois P, Jacobsen S, et al. Beneficial effect of triphenylphosphine on the bulk polymerization of L, L-lactide promoted by 2-ethylhexanoic acid tin (II) salt
[J]. Journal of Polymer Science Part A Polymer Chemistry, 1999, 37(14): 2413-2420.

[9] 郭晓东，全大萍，闫玉华，等.可吸收羟基磷灰石/聚DL-乳酸骨替换材料机械强度和生物降解性研究
[J].中国生物医学工程学报，2001, 20(1):23.

[10] 滕新荣，顾书英，任杰.超临界CO2中制备聚乳酸/羟基磷灰石复合支架材料
[J].材料导报，2005，19(9):114-117.