脐带血血浆对包皮成纤维细胞生长影响的实验研究

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (5): 548-551.

脐带血血浆对包皮成纤维细胞生长影响的实验研究

丁妍^1,2，　卢智勇²，　袁雅红²，　梦淑艳²，　冯静波²，　李东升²，　曾毅¹

1. 北京工业大学生命科学与生物工程学院，北京 100124；　2. 湖北医药学院附属太和医院生命科学研究所，湖北十堰 442000

收稿日期:2012-03-21 出版日期:2012-09-25 发布日期:2012-09-29
通讯作者: 曾毅，院士，博士生导师，Tel:(010) 67391441，Email：zengy@public.bta.net.cn 李东升，教授，博士生导师，Tel: (0719)8801418，Email：dsli1698@yahoo.com.cn E-mail:dyywzx@163.com
作者简介:丁妍(1979-)，女，讲师，博士研究生，研究方向：干细胞临床应用，Tel: (0719)8801137
基金资助:
国家“973”基金资助项目（2009CB930201）

The effect of human umbilical cord plasma on the growth of human foreskin fibroblast

DING Yan^1,2, LU Zhi-yong², YUAN Ya-hong², MENG Shu-yan², FENG Jing-bo², LI Dong-sheng², ZENG Yi¹

1. College of Life Science, Beijing University of Technology, Beijing 100124,China; 2. Life Science Institute, Hubei University of Medicine affiliated Taihe Hostipal, Shiyan Hubei 442000，China

Received:2012-03-21 Online:2012-09-25 Published:2012-09-29

摘要/Abstract

摘要：

目的　探讨人脐带血血浆（HUP）能否维持人包皮成纤维细胞（HFF）的生长。方法　将脐血浆经盐析、透析等处理后，按10%体积成分培养HFF，并与同样浓度的胎牛血清（FBS）进行比较，观察细胞的贴壁速度、生长曲线、细胞周期及增殖能力。结果　 HFF在含10%HUP的培养液中能长期生长，其生长生长形态、细胞增殖能力及特征都与在含同体积FBS的培养液中无显著差异。结论　人HUP能维持HFF的长期生长。

关键词: 脐血, 包皮成纤维细胞, 胎牛血清

Abstract:

Objective　To investigate whether human umbilical cord plasma (HUP) can be used to culture human foreskin fibroblast (HFF) cell.　Methods　Surplus HUP were collected in this study. After being treated by salting out and diasysis, the HUP were used to culture HFF as 10% volume, and compared the effects with FBS. The features, growth curve, cell cycle and reproductive activity of HFF were observed.　Results　compared to fetal bovine serum (FBS), the morphous, reproductive activity and fibroblast characteristic of HFF cell cultured in HUP were not distinctively different.　Conclusions　HUP can be used to culture HFF.

Key words: Cord blood, Foreskin fibroblast, Fetal bovine serum

中图分类号:

R338

丁妍, 卢智勇, 袁雅红, 梦淑艳, 冯静波, 李东升, 曾毅. 脐带血血浆对包皮成纤维细胞生长影响的实验研究[J]. 中国临床解剖学杂志, 2012, 30(5): 548-551.

DING Yan, LEI Zhi-Yong, YUAN Ya-Gong, MENG Chu-Yan, FENG Jing-Bei, LI Dong-Sheng, CENG Yi. The effect of human umbilical cord plasma on the growth of human foreskin fibroblast[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(5): 548-551.

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