中晚期孕期束缚应激对子代下丘脑多巴胺能神经元发育的影响

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (6): 663-666.

中晚期孕期束缚应激对子代下丘脑多巴胺能神经元发育的影响

郝彦利¹，　陈英华²，　董为人²

1.广州医学院人体解剖学教研室，广州 510180； 2.南方医科大学组织胚胎学教研室, 广州 510515

收稿日期:2012-07-28 出版日期:2012-11-25 发布日期:2012-12-11
作者简介:郝彦利（1973-），女，河北邯郸人，副教授，主要从事神经发育和再生的研究
基金资助:
广州市属高校科技、社科项目 (08A036)

Effects of prenatal stress on the development of dopaminergic neurons in the hypothalamus of offspring

HAO Yan-li¹, CHEN Ying-hua², DONG Wei-ren²

1.Department of Anatomy, Guangzhou Medical University, Guangzhou 510180, China； 2.Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China

Received:2012-07-28 Online:2012-11-25 Published:2012-12-11

摘要/Abstract

摘要：

目的　研究中晚期孕期束缚应激对子代下丘脑多巴胺能神经元发育的影响。方法　使用怀孕SD大鼠，实验组在孕期第15~21 d进行束缚应激，对照组则不受任何干扰。子代大鼠出生的当天为出生后的第0 天。在子代大鼠出生后的第1天（P1），第7天（P7）和第30天（P30）,分别测子代大鼠的体重，通过免疫组织化学染色和Western Blotting，观察和比较下丘脑内合成多巴胺的关键酶酪氨酸羟化酶（TH）染色像素密度及TH蛋白水平变化。结果　与正常对照组相比，中晚期孕期束缚应激子代大鼠在P1体重较低，下丘脑TH染色像素密度和TH蛋白水平上调，这种异常在P7和P30消失。结论　孕期应激使子代下丘脑多巴胺神经元早期发育异常, 可能是造成子代神经系统行为学异常的原因之一。

关键词: 孕期应激, 子代, 下丘脑, 多巴胺能神经元

Abstract:

Objective　To investigate the effect of prenatal stress on the development of dopaminergic neurons in hypothalamus of rat offspring.　Methods　Restraint stress was performed three times each day for pregnant SD rats from embryonic day 12-19 in prenatal stress group. The day that the offspring was born was defined as the postnatal day 1. Dopamine expression in the hypothalamus of offspring was observed using the method of immunostaining on postnatal day 1, 7 and 30, respectively.　Results　Body weight of offspring rats in prenatal stress group significantly decreased, while the pixel intensity and level of dopamine in hypothalamus was up-regulated, compared with control group at the postnatal day 1(P<0.05). This change disappeared at the postnatal day 7 and 30.　Conclusions　Prenatal stress had an effect on the prenatal development of dopaminergic neurons in the hypothalamus, which may induce the long-term detrimental consequences for brain function.

Key words: Prenatal stress, Offspring, Hypothalamus, Dopaminergic neurons

中图分类号:

R715.3
R324

郝彦利, 陈英华, 董为人. 中晚期孕期束缚应激对子代下丘脑多巴胺能神经元发育的影响[J]. 中国临床解剖学杂志, 2012, 30(6): 663-666.

HAO Pan-Li, CHEN Yang-Hua, DONG Wei-Ren. Effects of prenatal stress on the development of dopaminergic neurons in the hypothalamus of offspring[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(6): 663-666.

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