四种固定液对肾上腺组织冰冻切片固定效果的比较

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (3): 357-359.

四种固定液对肾上腺组织冰冻切片固定效果的比较

张利朝^1,2，　陈英华¹，　赵永斌²，　胡卫列²

1.南方医科大学组织胚胎学教研室，广州 510515； 2.广州军区广州总医院泌尿外科，广州 510010

收稿日期:2013-01-03 出版日期:2013-05-25 发布日期:2013-06-06
通讯作者: 胡卫列，教授，博士生导师，Tel: (020)88653533，E-mail: gz_urology@yahoo.cn E-mail:mrchch@126.com
作者简介:并列第一作者：张利朝（1977-），男，内蒙古呼和浩特人，在读博士，主要从事肾上腺疾病和微创泌尿外科研究，E-mail: lichaoz@21cn.com 陈英华（1978-），女，新疆喀什人，在读博士，主要从事组织工程及皮肤相关信号转导通路研究，E-mail: mrchch@126.com
基金资助:
广东省医学科研基金（B2010262）

Comparison of fixation effects of frozen section of adrenal glands fixed by four fixatives

ZHANG Li-chao^1,2 , CHEN Ying-hua¹, ZHAO Yong-bin², HU Wei-lie²

1.Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China; 2.Department of Urology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010,China

Received:2013-01-03 Online:2013-05-25 Published:2013-06-06

摘要/Abstract

摘要：

目的　以肾上腺组织为例观察比较丙酮、4%多聚甲醛、AAF和95%乙醇四种固定液的固定效果，为临床做出更好的快速冰冻切片，及时、准确的做出病理诊断提供实验基础。方法　取人正常肾上腺组织，冰冻切片后分别用四种固定液进行固定，然后行HE染色，观察各组的组织结构、细胞核染色、细胞质染色和核质对比度情况。结果　经冷丙酮固定的肾上腺组织切片，镜下组织结构清晰，高倍镜下观察：细胞核形态清晰完整，核染色和胞质染色清晰对比度好；经4%多聚甲醛固定的切片，镜下组织结构不清，细胞核形态较清晰，核染色和胞质染色对比度差；经AAF液固定的组织切片，镜下组织结构尚清晰，细胞核部分溶解，核染色均一，胞质染色较清晰；经95%乙醇固定的组织，组织结构尚清晰，核溶解普遍，胞质染色不清晰。结论　四种固定液都可用于固定肾上腺组织及其它细胞成分较多的病理肿瘤组织，但冷丙酮效果最好。

关键词: 固定液, 冰冻切片, H.E.染色

Abstract:

Objective　Comparing the fixation effects of four fixatives，acetone ,4% formaldehyde, AAF and 95% alcohol for adrenal glands, so as to provide experimental proof for making rapid and high quality frozen section clinically.　Methods 　Human normal adrenal glands were subjected to the technique of frozen section and then followed by different fixation and H.E. staining. The structures of all H.E. stained frozen sections of adrenal glands were examined carefully.　Results　Specimens fixed by acetone showed good effect with no structure contraction. The group fixed by 4% formaldehyde exhibited unclear structures observed by low magnification. The group fixed by AAF exhibited both clear structures and good staining effects ,however, partial nucleus shrinkage was observed. Specimens fixed by 95% alcohol showed unclear nuclear structure generally.　Conclusions　 These four fixatives could be used for all swelling tissue similar to adrenal glands with the standing out as best fixative.

Key words: Fixative, Frozen section, HE staining

中图分类号:

R361.2

张利朝, 陈英华, 赵永斌, 胡卫列. 四种固定液对肾上腺组织冰冻切片固定效果的比较[J]. 中国临床解剖学杂志, 2013, 31(3): 357-359.

ZHANG Li-Chao, CHEN Yang-Hua, DIAO Yong-Bin, HU Wei-Li. Comparison of fixation effects of frozen section of adrenal glands fixed by four fixatives[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 357-359.