凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性

中国临床解剖学杂志 ›› 2013, Vol. 31 ›› Issue (5): 560-563.

凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性

方志启¹,　吴刚¹,　王贺彬¹, 　陈晓宇²

1.合肥市第三人民医院泌尿外科　安徽医科大学附属临床学院，合肥 230021;
2.安徽医科大学组织胚胎学教研室, 合肥 230032

收稿日期:2013-03-15 出版日期:2013-09-25 发布日期:2013-10-16
通讯作者: 陈晓宇,博士，副教授，Tel: 0551-65161135 E-mail:fzq1022@163.com
作者简介:方志启(1969-)，男，安徽舒城人，硕士，副主任医师，主要从事泌尿外科临床基础方面的研究
基金资助:
安徽省自然科学基金资助项目(1208085MC53)

Correlation of apoptosis genes of p53，bcl-2 and bax in tissue of prostate

FANG Zhi-qi¹, WU Gang¹, WANG He-bing¹, CHEN Xiao-yu²

1.Department of Urology, the Third People Hospital of Hefei, Hefei 230022, China; 2.Department of Histology and Embryology, Anhui Medical University, Hefei 230032，China

Received:2013-03-15 Online:2013-09-25 Published:2013-10-16

摘要/Abstract

摘要：

目的　探讨细胞凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性。方法　收集36例前列腺癌(prostate caner, PCa)、20例前列腺增生(benign prostatic hyperplasia,BPH)和11例正常前列腺(normal prostatic, NP)，应用免疫组织化学S-P法检测凋亡相关基因p53、bcl-2、bax蛋白的表达。结果　①PCa和BPH组bcl-2蛋白阳性表达率明显高于NP组（P<0.05），而PCa组与BPH组阳性率差异无显著性。PCa组p53蛋白阳性表达率明显高于BPH组和NP组（P<0.01），而BPH组与NP组阳性率无显著性差异（P>0.05）。②p53与PCa分级有关，随着肿瘤分级增高而呈正相关（P<0.05）；bcl-2与PCa分级有关，随着肿瘤分级增高而呈正相关（P<0.01），显示bcl-2、p53蛋白表达随着病理分级的增高而增高。PCa、BPH和NP中bax阳性表达率差异无显著性。③p53蛋白表达阳性率≤5年生存组明显高于>5年生存组，呈负相关（P<0.05）；bcl-2、bax蛋白表达与生存期无关（P>0.05）。结论细胞凋亡相关基因 p53、bcl-2、bax蛋白的异常表达与PCa的发生和发展、病理分级和预后有相关性。　　

关键词: 前列腺癌, 基因, bcl-2, 免疫组织化学, p53, bax

Abstract:

Objective　To investigate clinical significance and expression between apoptosis and the expression of the related genes of p53，bcl-2 and bax in prostate cancer (PCa).　Methods　The expression of bcl-2, bax and p53 protein and apoptosis cells were detected by immunohistochemical S-P method respectively in tissues of 36 cases of PCa, 20 BPH and 11 NP. Results ① It showed that bcl-2 expression in Pca was significantly higher than that in NP and BPH (P<0.05), and there was no significant difference between PCa and BPH(P>0.05). p53 expression in PCa was significantly higher than that in BPH and NP (P<0.01), and there was no significant difference between BPH and NP(P>0.05). ② In the tissue of Pca， p53 and bcl-2 expression was positively correlated with pathological grade, as PCa increased in grades (P<0.05, P<0.01).It showed that expression levels of bcl-2 and p53 protein in PCa tissues increased with the progression of pathological grade. Bax positive expressing rate difference had no significance in PCa, BPH and NP. ③The expression of p53 was frequently detected in shorter-than-5-year survival group, than it was in longer-than-5-year survival group(P<0.05). That of bcl-2 expression was not related to survival period(P>0.05). Conclusion The over-expression of apoptosis-related genes: bcl-2，bax and p53 might be related to the carcinogenesis，progression pathological grade and prognosis of prostate carcinoma

Key words: Prostatic neoplasmas, Genes, bcl-2, Immunohistochemistry, p53, Bax

中图分类号:

方志启, 吴刚, 王贺彬, 陈晓宇. 凋亡相关基因p53、bcl-2、bax在前列腺组织中的相关性[J]. 中国临床解剖学杂志, 2013, 31(5): 560-563.

FANG Zhi-Qi, TUN Gang, WANG He-Ban, CHEN Xiao-Yu. Correlation of apoptosis genes of p53，bcl-2 and bax in tissue of prostate[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(5): 560-563.

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