脑出血后大鼠神经干细胞增殖及迁徙途径的实验研究

doi:10.13418/j.issn.1001-165x.2015

中国临床解剖学杂志 ›› 2015, Vol. 33 ›› Issue (2): 189-192.doi: 10.13418/j.issn.1001-165x.2015

脑出血后大鼠神经干细胞增殖及迁徙途径的实验研究

汤华军¹，　范光碧¹，　郑宇杰¹，　邓莉²，　高小青²，　杨朝鲜²

泸州医学院 1.人体解剖学教研室， 2.神经生物学研究室，四川泸州 646000

收稿日期:2014-05-05 发布日期:2015-04-21
通讯作者: 杨朝鲜，男，教授，硕士生导师，E-mail：lyycx@foxmail.com E-mail:337578364@qq.com
作者简介:汤华军（1977-），男，四川南充人，硕士，讲师，主要从事神经损伤与修复的研究，Tel：15082092936
基金资助:
泸州医学院青年基金项目（2011037）；泸州市科技局基金项目（泸市科【2013】134号）

The experimental study on proliferation and migration pathway of the neural stem cells in the rats following intracerebral hemorrhage

TANG Hua-jun¹, FAN Guang-bi¹, ZHENG Yu-jie¹, DENG Li², GAO Xiao-qing², YANG Chao-xian²

1. Department of Anatomy,Luzhou Medical College, Luzhou 646000, China; 2. Department of Neurobiology, Luzhou Medical College, Luzhou 646000, China

Received:2014-05-05 Published:2015-04-21

摘要/Abstract

摘要：

目的　探讨脑出血后大鼠神经干细胞增殖变化及迁徙途径。方法将75只SD大鼠随机分为脑出血组、假手术组和正常组，脑出血组采用自体血注入法复制脑出血模型。各实验大鼠腹腔注射溴脱氧核苷尿嘧啶（BrdU）标记神经干细胞，运用免疫组化染色观察神经干细胞增殖及迁徙分布情况。结果脑出血组侧脑室下区室管膜上皮的BrdU阳性细胞数比假手术组和正常组显著增加（P<0.05），BrdU阳性细胞沿胼胝体迁徙，最后散在分布于出血灶及周边区。结论脑出血后神经干细胞可增殖并沿胼胝体向出血灶及周边区迁徙，参与出血性脑损伤神经功能的修复。

关键词: 脑出血, 神经干细胞, 增殖, 迁徙途径

Abstract:

Objective To investigate proliferation and migration pathways of the neural stem cells in the rats following intracerebral hemorrhage (ICH). Methods The 75 SD rats were randomly divided into the ICH group, sham group and normal group the ICH group received injection of autologous blood to establish the ICH model. The bromodeoxyuridine (BrdU) was used to mark neural stem cells. Proliferation, migration and distribution of neural stem cells were observed by immunohistochemical staining. Results BrdU-positive cells located in the ependymal epithelium of subventricular zone increased significantly in the ICH group compared with the control group and normal group( P<0.05), and migrated along corpus callosum, and finally settled in the hemorrhagic focus and the surrounding area. Conclusion Neural stem cells in the rats following ICH can proliferate and migrate to the hemorrhagic focus and surrounding area along corpus callosum, and participate in repairing the nerve function of hemorrhagic brain injury.

Key words: Intracerebral hemorrhage, Neuron stem cells, Proliferation, Migration pathway

中图分类号:

R322.81

汤华军, 范光碧, 郑宇杰, 邓莉, 高小青, 杨朝鲜. 脑出血后大鼠神经干细胞增殖及迁徙途径的实验研究[J]. 中国临床解剖学杂志, 2015, 33(2): 189-192.

SHANG Hua-Jun, FAN Guang-Bi, ZHENG Yu-Jie, DENG Chi, GAO Xiao-Jing, YANG Chao-Xian. The experimental study on proliferation and migration pathway of the neural stem cells in the rats following intracerebral hemorrhage[J]. Chinese Journal Of Clinical Anatomy, 2015, 33(2): 189-192.

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脑出血后大鼠神经干细胞增殖及迁徙途径的实验研究

The experimental study on proliferation and migration pathway of the neural stem cells in the rats following intracerebral hemorrhage

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