关键词: 单羧酸转运蛋白1、2、4, 急性心衰, 应激, 肾上腺

Abstract:

Objective   To observe the alterations of the MCT 1、2、4 expression in adrenal gland of rats with acute heart failure , so as to explore the alterations of energy metabolism of adrenal gland under stress of acute heart failure.   Method     Fifty healthy adult SD rats were randomly divided into 3 groups: normal, sham(2 subgroups) and experimental groups(2 subgroups). In experimental group, acute heart failure model was established by 1.5% sodium pentobarbital injection i.v . In sham group, the rats underwent the same surgical procedures as those in experimental group with NS instead of 1.5% sodium pentobarbital injection. After injection, left ventricular systolic pressure（LVSP）and increased rate of left ventricular pressure maximum（+dp/dtmax）were recorded to judge whether the model was successful or not. The animals were sacrificed and their adrenal glands were properly taken at 10 and 20 min after the models had been established successfully. Immunohistochemistry（IHC） and Western Bolt(WB) methods were used to localize and detect the alterations of MCT1、2、4 expression.   Results   IHC and WB results showed that the expressions of MCT1、2、4 were statistically increased in both experimental and sham groups in 10 min(P<0.05), compared with the normal group. The semi-quantitative analysis of MCT1、2、4 showed that in 20min the amount of MCT1、2、4 expression in experimental group was lower than that in control group (MCT1:P<0.05; MCT2 & MCT4: P>0.05).   Conclusions   Under the conditions of  acute heart failure and traumatic stress, the upregulation of MCT1、2、4 expression in adrenal gland might make a compensation for energy consumption within 10 min. However, the capacity of compensation was limited. The results suggest that MCT1、2、4 might play an important role in stress reaction .

Key words: MCT1、2、4, Acute Heart Failure, Stress, Adrenal gland