去卵巢联合糖皮质激素诱发兔骨质疏松模型的研究

doi:10.13418/j.issn.1001-165x.201

中国临床解剖学杂志 ›› 2015, Vol. 33 ›› Issue (2): 204-208.doi: 10.13418/j.issn.1001-165x.201

去卵巢联合糖皮质激素诱发兔骨质疏松模型的研究

张远华¹，苏艳杰²，黄潮桐³

1.广州市番禺区南村医院，广州511442； 2.香港中文大学深圳研究院，深圳518057；
3.广东医学院附属厚街医院，广东东莞 523945

收稿日期:2014-12-04 发布日期:2015-04-21
通讯作者: 黄潮桐，教授，主任医师，硕士生导师，E-mail： myhct@medmail.com.cn E-mail:mail:yuanhua9901186@163.com
作者简介:张远华（1980-），男，湖北省黄冈市人，医学硕士，主治医师，研究方向：骨质疏松与数字骨科学，Tel：13543436469

The research of ovariectomy combined with glucocorticoid for induction of osteoporosis in rabbits

ZHANG Yuan-hua¹, SU Yan-jie², HUANG Chao-tong³

1.Nancun Hospital of Panyu District, Guangzhou 511442, China; 2.The Chinese University of Hong Kong Shenzhen Research, Shenzhen 518057, China; 3.Institute Affiliated Houjie Hospital of Guangdong Medical College, Dongguan 523945, China

Received:2014-12-04 Published:2015-04-21

摘要/Abstract

摘要：

目的　建立去卵巢联合糖皮质激素致兔骨质疏松模型，探讨去卵巢联合糖皮质激素对兔骨代谢的影响。方法将18只新西兰雌兔随机分为3组：假手术组（SHAM，生理盐水5 ml·kg-1·d-1）；去卵巢组（OVX，生理盐水5 ml/kg/d)；去卵巢联合糖皮质激素组（OVX+GC，地塞米松1 mg·kg-1·d-1）。实验第0、3和6周，检测各组大白兔腰椎（3～6）和右侧股骨的骨密度。实验结束时，取右侧股骨进行生物力学检测；取第3腰椎进行骨组织形态计量学检测。结果与SHAM组相比，OVX+GC组大白兔骨密度、骨生物力学性能（最大载荷、断裂载荷等）、骨小梁面积百分数、骨小梁厚度和骨小梁数量均显著降低（P<0.05），骨小梁分离度显著升高（P<0.05），而OVX组大白兔的均无显著差异。结论去卵巢联合糖皮质激素法建立兔骨质疏松模型切实可行，而且效果优于单纯去卵巢兔骨质疏松模型。

关键词: 去卵巢, 糖皮质激素, 骨质疏松, 兔

Abstract:

Objective To explore the effect of ovariectomy combined with glucocorticoid on rabbits bone metabolism and develop a new method for inducing osteoporosis in rabbits. Method 18 New Zealand female rabbits were randomly divided into 3 groups: Sham-operated group (SHAM, Physiological saline 5 ml·kg-1·d-1); ovariectomy group (OVX, Physiological saline 5 ml·kg-1·d-1); ovariectomy combined with glucocorticoid group (OVX +GC, Dexamethasone 1 mg·kg-1·d-1). The femoral bone mineral density (BMD) of the rabbit lumbar (3~6) and the right femoral were assessed at 0, 3 and 6-week, respectively. At the end of the experiment, the right femur biomechanics and the third lumbar vertebra bone tissue morphology metrology were determined. Result Compared with Sham-operated group, the BMD, the bone biomechanics (maximum load, fracture load) , the trabecular bone area percentage, the bone trabecular thickness and the bone trabecular number in the ovariectomy combined with glucocorticoid group were significantly decreased (P<0.05), the bone separating degree were significantly increased(P<0.05), while the ovariectomy group was not significantly different. Conclusion The method of ovariectomy combined with glucocorticoid is better at induction of osteoporosis in rabbits than ovariectomy alone and it is a feasible approach.

Key words: Ovariectomy, Glucocorticoid, Osteoporosis, Rabbits

中图分类号:

R965.2

张远华, 苏艳杰, 黄潮桐. 去卵巢联合糖皮质激素诱发兔骨质疏松模型的研究[J]. 中国临床解剖学杂志, 2015, 33(2): 204-208.

ZHANG Yuan-Hua, SU Yan-Jie, HUANG Chao-Dong. The research of ovariectomy combined with glucocorticoid for induction of osteoporosis in rabbits[J]. Chinese Journal Of Clinical Anatomy, 2015, 33(2): 204-208.

参考文献

[1] Turner AS. Animal models of osteoporosis-necessity and limitations
[J]. Eur Cell Mater, 2001, 1:66–81.

[2] Kimmel DB. Animal models for in vivo experimentation in osteoporosis research
[M]// Marcus R, Feldman D, Kelsey J (eds). Osteoporosis. Academic Press, San Diego, 2001:2:29-47.

[3] 魏劲松, 龚颜, 王健, 等. 去卵巢大鼠骨髓微环境中RUNX2/PPARγ基因及OPG/RANKL基因蛋白表达变化的实验研究
[J]. 中国矫形外科杂志, 2013, 21(13):1359-1364.

[4] Zhou DA, Zheng HX, Wang CW, et al. Influence of glucocorticoids on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells
[J]. BMC Musculoskelet Disord, 2014, 15:239.

[5] Lin S, Huang J, Zheng L, et al. Glucocorticoid-induced osteoporosis in growing rats
[J]. Calcif Tissue Int, 2014, 95(4):362-73.

[6] Ke HZ. Animal models for osteoporosis research
[J]. Bone, 2010, 47(3Suppl):S349-S350.

[7] Cho DY, Sinha SR, Gardner JM, et al. Effect of intratonsillar injection of steroids on the palatine tonsils of rabbits
[J]. Laryngoscope, 2014, 124(12):2811-2817.

[8] Oheim R, Beil FT, Köhne T, et al. Sheep model for osteoporosis: sustainability and biomechanical relevance of low turnover osteoporosis induced by hypothalamic -pituitary disconnection
[J]. J Orthop Res, 2013,31(7):1067-1074.

[9] Castaneda S, Calvo E, Largo R, et al. Characterization of a new experimental model of osteoporosis in rabbits
[J]. J Bone Miner Metab, 2008, 26 (1): 53-59.

[10]Eberhardt AW, Yeager-Jones A, Blair HC. Regional trabecular bone matrix degeneration and osteocyte death in femora of glucocorticoid- treated rabbits
[J]. Endocrinology, 2001, 142 (3): 1333-1340.

[11]张智海, 刘忠厚, 李娜等. 中国人骨质疏松症诊断标准专家共识（第三稿•2014版）
[J]. 中国骨质疏松杂志, 2014, 20(9):1007-1010.

[12]Punda M, Grazio S. Bone densitometry-the gold standard for diagnosis of osteoporosis
[J]. Reumatizam, 2014, 61(2):70-74.

[13]Turner CH. Biomechanics of bone: determinants of skeletal fragility and bone quality
[J]. Osteoporos Int, 2002, 13 (2): 97-104.

[14]方忠，扬琴，李锋, 等. 去势法兔骨质疏松模型建立的探讨
[J].中国骨质疏松杂志,2005,11(2):202-209.

[15]Slyfield CR, Tkachenko EV, Wilson DL, et al. Three-dimensional dynamic bone histomorphometry
[J]. J Bone Miner Res, 2012, 27(2):486-495.

[16]马信龙, 马剑雄, 徐卫国, 等. 骨科生物力学研究的测量方法学专家共识
[J]. 中国骨质疏松杂志, 2014, 20(9):1039-1054.

[17] 张晓刚, 秦大平, 宋敏, 等. 骨生物力学的应用与研究进展
[J]. 中国骨质疏松杂志, 2012, 18(9): 850-860.

去卵巢联合糖皮质激素诱发兔骨质疏松模型的研究

The research of ovariectomy combined with glucocorticoid for induction of osteoporosis in rabbits

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	林杰, 金甬, 庞清江. 胃肠道菌群与骨质疏松症关系的研究进展[J]. 中国临床解剖学杂志, 2024, 42(1): 109-111.
[2]	李海艳, 司丽娜, 要星晨, 杜心如. 股骨颈滋养孔的形态特点及临床意义[J]. 中国临床解剖学杂志, 2023, 41(3): 258-262.
[3]	何艾岭, 王清, 李广州. 骨质疏松性椎体压缩骨折患者腰椎椎基静脉孔的CT影像解剖学研究[J]. 中国临床解剖学杂志, 2023, 41(3): 283-287.
[4]	黄星翔, 钟超, 叶华, 徐春贻, 刘云飞, 蒋璐. 基于Nrf2通路探讨松脂醇二葡萄糖苷改善小鼠骨质疏松的机制研究[J]. 中国临床解剖学杂志, 2023, 41(2): 162-171.
[5]	陈俊泽, 俞辉, 肖进. 雷公藤甲素通过抑制破骨细胞生成预防骨丢失的研究[J]. 中国临床解剖学杂志, 2023, 41(1): 77-80.
[6]	马学强, 曹林忠, 蒋玮, 张琪, 刘孟初, 王多贤. 生骨再造丸对兔激素性股骨头坏死骨形态计量学指标的影响[J]. 中国临床解剖学杂志, 2022, 40(5): 555-561.
[7]	姚文君, 李才, 许刚, 叶雨辰, 朱坤, 王艳丽, 张长春. 重度骨质疏松条件下后路枢椎三种内固定方式的有限元分析[J]. 中国临床解剖学杂志, 2022, 40(2): 198-203.
[8]	邓博, 徐庶钦, 王方舟, 石宇航, 李益行, 吝怡, 樊茜, 吉琳, 陈雯雯, 吕毅, 严小鹏. 磁压榨技术治疗输尿管远端梗阻的实验研究[J]. 中国临床解剖学杂志, 2021, 39(3): 319-322.
[9]	张世浩, 谭亮, 林云志, 王洪, 方国芳, 桑宏勋. OLIF联合皮质骨轨迹螺钉治疗腰椎退行性疾病的临床疗效分析[J]. 中国临床解剖学杂志, 2020, 38(2): 198-201.
[10]	林琼萍, 李达飞, 杨静雅, 徐飘, 张珊珊, 田广永. 耳内镜下新西兰兔中耳的解剖研究[J]. 中国临床解剖学杂志, 2020, 38(1): 14-18.
[11]	杨阳, 武文斌, 刘斌, 张良明, 罗春晓, 戎利民. 高盐饮食通过激活破骨细胞促进小鼠骨质疏松发生[J]. 中国临床解剖学杂志, 2019, 37(3): 267-272.
[12]	查丁胜, 　徐武岩, 　黄嘉文, 　李亚骐, 　陈诗君, 　林振, 　林宏生, 　查振刚, 　吴昊. 经皮椎体成形术骨水泥渗漏的回顾性分析[J]. 中国临床解剖学杂志, 2019, 37(3): 316-321.
[13]	查丁胜,邓轩赓,陈建庭. 低强度高频率振动对成骨细胞生物学特性的影响[J]. 中国临床解剖学杂志, 2018, 36(2): 165-173.
[14]	李建赤,刘岗,薛小华,谭加群,徐自强,吴春辉. 老年人股骨转子间骨折PFNA和DHS内固定术疗效的临床研究[J]. 中国临床解剖学杂志, 2017, 35(5): 571-575.
[15]	刘俊豪,姚欣强,黄祖成,黄志平,吴晓亮,吴秀华,季伟,孔刚刚,李榕,朱青安. 兔颈脊髓半侧挫伤模型的建立及其MRI和组织学表现的研究[J]. 中国临床解剖学杂志, 2017, 35(1): 31-36.