去卵巢联合糖皮质激素诱发兔骨质疏松模型的研究
The research of ovariectomy combined with glucocorticoid for induction of osteoporosis in rabbits
目的 建立去卵巢联合糖皮质激素致兔骨质疏松模型，探讨去卵巢联合糖皮质激素对兔骨代谢的影响。 方法 将18只新西兰雌兔随机分为3组：假手术组（SHAM，生理盐水5 ml·kg-1·d-1）；去卵巢组（OVX，生理盐水5 ml/kg/d)；去卵巢联合糖皮质激素组（OVX+GC，地塞米松1 mg·kg-1·d-1）。实验第0、3和6周，检测各组大白兔腰椎（3～6）和右侧股骨的骨密度。实验结束时，取右侧股骨进行生物力学检测；取第3腰椎进行骨组织形态计量学检测。 结果 与SHAM组相比，OVX+GC组大白兔骨密度、骨生物力学性能（最大载荷、断裂载荷等）、骨小梁面积百分数、骨小梁厚度和骨小梁数量均显著降低（P<0.05），骨小梁分离度显著升高（P<0.05），而OVX组大白兔的均无显著差异。 结论 去卵巢联合糖皮质激素法建立兔骨质疏松模型切实可行，而且效果优于单纯去卵巢兔骨质疏松模型。
Objective To explore the effect of ovariectomy combined with glucocorticoid on rabbits bone metabolism and develop a new method for inducing osteoporosis in rabbits. Method 18 New Zealand female rabbits were randomly divided into 3 groups: Sham-operated group (SHAM, Physiological saline 5 ml·kg-1·d-1); ovariectomy group (OVX, Physiological saline 5 ml·kg-1·d-1); ovariectomy combined with glucocorticoid group (OVX +GC, Dexamethasone 1 mg·kg-1·d-1). The femoral bone mineral density (BMD) of the rabbit lumbar (3~6) and the right femoral were assessed at 0, 3 and 6-week, respectively. At the end of the experiment, the right femur biomechanics and the third lumbar vertebra bone tissue morphology metrology were determined. Result Compared with Sham-operated group, the BMD, the bone biomechanics (maximum load, fracture load) , the trabecular bone area percentage, the bone trabecular thickness and the bone trabecular number in the ovariectomy combined with glucocorticoid group were significantly decreased (P<0.05), the bone separating degree were significantly increased(P<0.05), while the ovariectomy group was not significantly different. Conclusion The method of ovariectomy combined with glucocorticoid is better at induction of osteoporosis in rabbits than ovariectomy alone and it is a feasible approach.
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