目的　探讨雌激素对hASCs成脂分化过程中脂滴相关特异基因DNA断裂因子相似蛋白C（The cell death-inducing DNA fragmentation 45-like effector, CIDEc）、脂滴包被蛋白（Perilipin1, Plin1）mRNA表达的影响。  方法　对３例患者行脂肪抽吸术，并进行hASCs培养及传代，鉴定表面标志物；经典鸡尾酒法诱导其成脂，不同浓度(10-8、10-7、10-6 mol/L)17β-雌二醇(17β-E2)作用于细胞，分别于24 h、4、7、11d收获细胞，用RT-PCR检测CIDEc、Plin1 mRNA表达情况。  结果　各组细胞经药物作用后，脂肪细胞中CIDEc及Plin1 mRNA的表达水平随着17β-E2浓度的升高而下降；而随着作用时间的延长，CIDEc及Plin1 mRNA表达水平分别于7 d和4 d达到高峰期，其后逐渐下降。当17β-E2浓度为10-6 mol/L时，细胞中CIDEc及Plin1 mRNA的表达水平最低，差异有统计学意义(P＜0.01)；而加入雌激素受体阻滞剂ICI182780的一组细胞，雌激素对CIDEc 及Plin1 mRNA表达的抑制作用明显减弱。  结论　17β-E2能够显著降低脂肪细胞分化过程中CIDEc及Plin1的mRNA表达,并可能通过这一途径抑制脂肪细胞内脂滴的增殖。

Objective   To evaluate the effect of different concentrations of 17β-estradiol（17β-E2）on adipocytes lipid droplet growth and CIDEc, Plin1 mRNA expression in vitro.    Methods    Human primary adipose tissue-derived stem cells(hASCs) were isolated from liposuction aspirates of three patients and cultured, passaged and  identified the cell surface markers. hASCs were induced differentiated at 0 mol/L(17β-E2 free DMEM/F12),10-8 mol/L, 10-7 mol/L and 10-6 mol/L 17β-E2 and ICI182780 was added to culture human primary preadipocytes. The expression of CIDEc and Plin1 mRNA was detected by RT-PCR after staining with oil red O.  Result   Different concentrations of 17β-E2 suppressed CIDEc mRNA and Plin1 mRNA expression in human primary preadipocytes. The expression peak of CIDEc and Plin1 mRNA occurred at 7 and 4 days.  At 10-6 mo/L 17β-E2，the expression of CIDEc and Plin1 mRNA were the lowest. Andwith the increase in 17β-E2 concentration，the expression of CIDEc and Plin1 mRNA was in decline.  Conclusion   17β-E2 reduces CIDEc and Plin1 mRNA expression in adipocytes，in which inhibited CIDEc and Plin1 expression may contribute to the negative effect of 17β-E2 on lipid droplets.