人参皂苷Rbl对大鼠局灶性脑缺血白质重塑的影响

doi:10.13418/j.issn.1001-165x.2015.04.017

中国临床解剖学杂志 ›› 2015, Vol. 33 ›› Issue (4): 451-454.doi: 10.13418/j.issn.1001-165x.2015.04.017

人参皂苷Rbl对大鼠局灶性脑缺血白质重塑的影响

胡光强¹，　张慧³，　萧洪文¹，　高小青⁴，　杨朝鲜⁴，　余录²

1.泸州医学院解剖学教研室，四川泸州 646000；　2泸州医学院基础医学院，四川泸州 646000； 3.乐山市
第五人民医院药剂科，四川乐山 614900； 4.泸州医学院神经生物学研究室，四川泸州 646000

收稿日期:2014-01-01 出版日期:2015-07-25 发布日期:2015-08-14
通讯作者: 余录，实验师，E-mail：yulu863@sina.com
作者简介:胡光强（1970-），男，副教授，专业方向：神经解剖学，E-mail：hgq863@sina.com
基金资助:
泸州医学院青年基金（201125）

Effect of ginsenoside Rbl on white matter remodeling in rats with focal cerebral ischemia

HU Guang-qiang¹, ZHANG Hui³, XIAO Hong-wen¹, GAO Xiao-qing⁴, YANG Chao-xian⁴, YU Lu²

1.Department of Anatomy, Luzhou Medical College，Luzhou 646000，China；2. School of Basic Medical Sciences，Luzhou Medical College，Luzhou 646000，China；3. Department of Pharmacy, Leshan Fifth People's Hospital，Leshan 614900，China；4. Department of Neurobiology, Luzhou Medical College, Luzhou 646000, China

Received:2014-01-01 Online:2015-07-25 Published:2015-08-14

摘要/Abstract

摘要：

目的　探讨人参皂苷Rbl(Ginsenoside Rb1，GSRb1)对大鼠局灶性脑缺血白质重塑的影响。方法　大鼠随机分为假手术组、溶媒处理组和人参皂苷Rbl处理组，采用线栓法建立大鼠大脑中动脉缺血再灌注（middle cerebral artery occlusion/reperfusion, MCAO/R）损伤模型，用LFB染色观察大鼠胼胝体和内囊的髓鞘变化，用免疫组化染色法检测缺血侧胼胝体GFAP和APP的表达以评估星形胶质细胞和轴突的改变。结果　缺血2 h再灌72 h后，溶媒处理组胼胝体和内囊有明显的髓鞘紊乱、脱失，胼胝体GFAP和APP表达显著增加。与溶媒处理组相比，GSRb1处理组髓鞘脱失有明显改善 (P<0. 01, P<0. 05)且胼胝体GFAP和APP表达显著减少(P<0. 05)。结论　GSRb1可能促进大鼠局灶性脑缺血后脑白质重塑。

关键词: 人参皂苷Rbl, 　局灶性脑缺血, 　白质损伤

Abstract:

Objective To investigate the effect of Ginsenoside Rbl (GSRb1) on white matter remodeling in rats with focal cerebral ischemia. Methods Rats were randomly divided into sham-operated group, vehicle-treated group and GSRb1-treated group, and MCAO/R (middle cerebral artery occlusion/reperfusion) model was induced by thread embolism; then pathological change of myelin sheath was observed by LFB staining in the cerebral corpus callosum and internal capsule, damage to astrocytes and axons was examined by GFAP and APP immunostaining. Results after 72 h following MCAO/R, obvious demyelination appeared in the corpus callosum and internal capsule and the expression of GFAP and APP was increased significantly in the corpus callosum. Compared with the vehicle-treated group, demyelination was significantly improved in GSRb1-treated group (P<0.01, P<0.05), and the expression GFAP and APP was greatly decreased in GSRb1-treated group (P<0.05). Conclusion Our findings suggest GSRb1 probably promote the white matter remodeling in rats with focal cerebral ischemia.

Key words: GSRbl, Focal cerebral ischemia, White matter remodeling

胡光强,张慧,萧洪文,高小青,杨朝鲜,余录. 人参皂苷Rbl对大鼠局灶性脑缺血白质重塑的影响[J]. 中国临床解剖学杂志, 2015, 33(4): 451-454.

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人参皂苷Rbl对大鼠局灶性脑缺血白质重塑的影响

Effect of ginsenoside Rbl on white matter remodeling in rats with focal cerebral ischemia

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