脊髓损伤后5-HT2A受体的变化与鼠尾痉挛关系的分析

doi:10.13418/j.issn.1001-165x.2016.01.021

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (1): 83-86.doi: 10.13418/j.issn.1001-165x.2016.01.021

脊髓损伤后5-HT2A受体的变化与鼠尾痉挛关系的分析

王永为¹，　李海艳¹，　高亚贤²，　郭森¹ ，　谢红林¹

承德医学院 1.人体解剖学教研室，　2. 免疫学教研室，河北承德 067000

收稿日期:2015-05-25 出版日期:2016-01-25 发布日期:2016-01-28
作者简介:王永为（1984-），男，硕士，主要从事临床应用解剖学及脊髓损伤与修复研究, Tel：15512367286，E-mail:wangyongwei5116@163.com
基金资助:
河北省卫生厅自然科学基金（20150016）

Analysis of the relationship between the changes of 5-HT2A receptor in spinal cord and the mouse tail spasm after spinal cord injury

WANG Yong-wei¹, LI Hai-yan¹, GAO Ya-xian², GUO Sen¹, XIE Hong-lin¹

1. Department of Anatomy, Chengde Medical University, Chengde, HeBei 067000, China; 2. Department of Immunity, Chengde Medical University,Chengde, HeBei 067000, China

Received:2015-05-25 Online:2016-01-25 Published:2016-01-28

摘要/Abstract

摘要：

目的　通过损伤大鼠脊髓腰1节段来探讨损伤下段5-HT2A受体免疫反应变化与鼠尾痉挛状态的关系，为治疗患者脊髓损伤后肢体痉挛提供理论依据。方法　60只雄性Wistar大鼠随机分为空白组、打击组和横断组（n=20），用免疫组化法研究5-HT2A受体免疫反应的变化，并分析与鼠尾痉挛状态的关系。所得数据统计学处理。结果　(1)打击组和横断组5-HT2A受体免疫反应的密度比空白组增高，横断组增高更显著，差异有统计学意义。(2)脊髓损伤后鼠尾痉挛状态与5-HT2A受体的密度和脊髓损伤的程度呈正相关。结论　脊髓损伤后鼠尾痉挛状态与5-HT2A受体密度的变化呈正相关，应用5-HT2A受体阻滞剂治疗患者的肢体痉挛提供了理论依据。

关键词: 脊髓损伤, 5羟色胺2A受体, 痉挛状态

Abstract:

Objective　To study the relationship between the immunoreactive changes of 5-HT2A receptor and tail Spasticity, within spinal L1 segment after SCI, and to provide a theoretical basis for The treatment of spinal cord injury.　Methods　Sixty adult males Wistar rats were randomly separated into three groups: control group, attacked group and transversed group. To study 5-HT2AR immunoreactivity and hyperexcitability of tail of rats. Results (1) Compared the attacked and transversed groups with the control group, 5-HT2AR-IR density significantly increased in both attacked and transversed groups compared with that in the control group. (2) Spasticity and reflective hyperfunction symptom after SCI were positively correlated with 5-HT2AR-IR density and extent of SCI. Conclusion Spasticity and reflective hyperfunction symptom after SCI are positively correlated wtih 5-HT2AR-IR density and the extent of SCI, which provides the theoretical basis for using 5-HT2A receptor blockers in patients with limb spasm.

Key words: Spinal cord injury, 5-HT2A receptor, Spasticity

王永为,李海艳,高亚贤,郭森,谢红林. 脊髓损伤后5-HT2A受体的变化与鼠尾痉挛关系的分析[J]. 中国临床解剖学杂志, 2016, 34(1): 83-86.

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脊髓损伤后5-HT2A受体的变化与鼠尾痉挛关系的分析

Analysis of the relationship between the changes of 5-HT2A receptor in spinal cord and the mouse tail spasm after spinal cord injury

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