钙激活的氯离子通道Anoctamin 5 在骨骼肌发育过程中的表达变化

doi:10.13418/j.issn.1001-165x.2016.03.017

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (3): 318-321.doi: 10.13418/j.issn.1001-165x.2016.03.017

钙激活的氯离子通道Anoctamin 5 在骨骼肌发育过程中的表达变化

宋海岩，　张毅敏，　周立，　田岳民，　付升旗

新乡医学院基础医学院人体解剖学教研室　河南省医用组织再生重点实验室，河南新乡 453003

收稿日期:2015-05-14 出版日期:2016-05-25 发布日期:2016-06-17
通讯作者: 田岳民，E-mail: zhouyike2009@163.com
作者简介:宋海岩(1980-)，女，河南新乡人，讲师，硕士, E-mail: shy80825@163.com
基金资助:
国家自然科学基金（31200858）;新乡医学院科研培育基金（2014QN119）

Developmental expression of calcium activated chloride ion channels Anoctamin5 in mouse skeletal muscle

SONG Hai-yan, ZHANG Yi-min, ZHOU Li, TIAN Yue-min, FU Sheng-qi

Department of Anatomy, School of Basic Medical Sciences, Xinxiang Medical University; Key Laboratory for Medical Tissue Regeneration of Henan Province, Xinxiang 453003, Henan

Received:2015-05-14 Online:2016-05-25 Published:2016-06-17

摘要/Abstract

摘要：

目的　探讨钙依赖的氯离子通道Anoctamin5在骨骼肌发育过程中的可能作用。方法　采用 RT-PCR 检测Anoctamin5 mRNA在小鼠骨骼肌发育的不同时期的表达变化，同时，采用免疫荧光，Western blot的方法检测Anoctamin 5蛋白在小鼠骨骼肌发育不同时期的表达变化。结果　Anoctamin5 mRNA 与蛋白在1 d 至6 月的小鼠骨骼肌中均有表达，但随着年龄的增长，Anoctamin5 mRNA 与蛋白的表达呈逐渐降低的趋势。结论　Anoctamin5在骨骼肌的发育过程中发挥着重要的作用，也可能参与骨骼肌的分化、增殖及损伤修复，这也与Anoctamin5 突变的病人出现症状较晚相一致。

关键词: Anoctamin5, 骨骼肌, 发育, 表达

Abstract:

Objective　To study the possible role of the calcium dependent Anoctamin5 chloride ion channels in the process of skeletal muscle development.　Methods　RT-PCR was used to detect Anoctamin5 mRNA expression in skeletal muscle of different development periods in mice, and immunofluorescence and Western blot methods was used to detect Anoctamin 5 protein expression in different development periods of mice skeletal muscle.　Results　Anoctamin5 mRNA and protein expression in skeletal muscle in mice from 1 day to 6 months, but with the growth of the age, Anoctamin5 mRNA and protein expression underwent a trend of gradual reduction.　Conclusion　Anoctamin5 plays an important role in the development process of skeletal muscle in mice, which may participate in differentiation, proliferation, injury and repair of skeletal muscle. Our foundings are also with consistent with the patient developed symptoms later caused by Anoctamin5 mutations.

Key words: Anoctamin5, Skeletalmuscle, Development, Expression

宋海岩,张毅敏,周立,田岳民,付升旗. 钙激活的氯离子通道Anoctamin 5 在骨骼肌发育过程中的表达变化[J]. 中国临床解剖学杂志, 2016, 34(3): 318-321.

SONG Hai-yan, ZHANG Yi-min, ZHOU Li, TIAN Yue-min, FU Sheng-qi . Developmental expression of calcium activated chloride ion channels Anoctamin5 in mouse skeletal muscle[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(3): 318-321.

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钙激活的氯离子通道Anoctamin 5 在骨骼肌发育过程中的表达变化

Developmental expression of calcium activated chloride ion channels Anoctamin5 in mouse skeletal muscle

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