丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究

doi:10.13418/j.issn.1001-165x.2016.04.014

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (4): 423-427.doi: 10.13418/j.issn.1001-165x.2016.04.014

丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究

杨拯，　李欣芮，　王强，　刘双，　黄倩，　计震华

成都医学院基础医学院基础医学实验教学中心，成都 610500

收稿日期:2015-09-22 出版日期:2016-07-25 发布日期:2016-08-03
作者简介:杨拯（1980-）, 男, 四川苍溪人, 硕士, 高级实验师,主要从事实验动物学的教学和脊髓损伤与胃肠道动力障碍疾病的治疗和机制研究，Tel:028-62739316, E-mail：yzixjj@163.com
基金资助:
四川省教育厅科研项目（13ZB0095）;成都医学院科研基金项目（CYZ12-012）

Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method

YANG Zheng, LI Xin-rui, WANG Qiang, LIU Shuang, HUANG Qian, JI Zhen-hua

Teaching Center of Basic Science, School of Basic Medical Science, Chengdu Medical College, Chengdu 610500,China

Received:2015-09-22 Online:2016-07-25 Published:2016-08-03

摘要/Abstract

摘要：

目的　探讨丝线法建立豚鼠不完全性小肠梗阻模型对ICC细胞表达c-kit的影响。方法　选取43只成年健康豚鼠随机分成正常A组、假手术B组、模型组（C、D、E）。对模型组豚鼠通过丝线法造成不完全性肠梗阻,3 d后取出丝线并关闭腹腔,分别于术后第1d（C组）,第3d（D组）,第7d（E组）对小肠收缩力、肠黏膜损伤程度及小肠c-kit含量变化进行测定。结果　模型组各项指标的测定与正常组比较均有统计学差异（P<0.05）,但E组肠黏膜损伤程度与A组比较无明显差异（P>0.05）。E组小肠收缩力较D组高（P<0.05），肠黏膜损伤程度较D组轻（P<0.01），小肠c-kit含量较D组明显增高（P<0.01）。结论　丝线法能建立豚鼠不完全性肠梗阻模型，同时可降低c-kit蛋白表达。

关键词: , Cajal间质细胞, 丝线法}c-kit蛋白}豚鼠}不完全性小肠梗阻

Abstract:

Objective　To investigatethe expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models in guinea pig with the silk method.　Methods　43 adult healthy guinea pigs were selected and randomly divided into the normal group A, sham-operated group B, model groups（C, D, E）. Model groups were established for incomplete small intestinal obstruction by the means of silk method for 3 days. After taking out of the silk and closing the abdominal cavity, the abdominal cavity was opened and the intestinal contractility was measured，respectively at 1st（C group）, 3rd（D group）,7th（E group）. Meanwhile, the contractility of intestinal tissue was measured, the degree of intestinal mucosal membrane injury was evaluated and the expression of c-kit was observed.　Results　The comparison of normal and model groups in every data’s measurement were significant on statistics (P<0.05),but the comparison of intestinal mucosal membrane injury between E and A group had no significant difference (P>0.05). The contractility of intestinal tissue of E group was higher than D group (P<0.05), and the degree of intestinal mucosal membrane injury was less serious than D group (P<0.01). Besides, the expression of c-kit in E group wass significantly higher than D group (P<0.01). Conclusion　Incomplete intestinal obstruction with down-regulated c-kit could be established by the silk method.

Key words: Interstitial cells of Cajal, Silk method, c-kit, Guinea pig, Incomplete small intestinal obstruction

杨拯，李欣芮，王强，刘双，黄倩，计震华. 丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究[J]. 中国临床解剖学杂志, 2016, 34(4): 423-427.

YANG Zheng, LI Xin-rui, WANG Qiang, LIU Shuang, HUANG Qian, JI Zhen-hua. Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(4): 423-427.

参考文献

[1] Wu CC, Lin YM, Gao JH, et al. Are interstitial cells of Cajal involved in mechanical stress-induced gene expression and impairment of smooth muscle contractility in bowel obstrucion[J]. 2013, PLoS ONE, 8(9):e76222.
[2] Wu B, Liu L, Gao H, et al. Distribution of interstitial cells of Cajal in Meriones unguiculatus and alterations in the development of incomplete intestinal obstruction[J]. Histol Histopathol, 2013, 28(12):1567-1575.
[3] 朱晓艳,李昆, 彭梅芳, 等. 电针足三里穴对不全肠梗阻大鼠小肠Cajal间质细胞影响的免疫组化观察[J]. 中国普外基础与临床杂志, 2012, 19(9): 984-987.
[4] El-Awady SI, El-Nagar M, El-Dakar M, et al. Bacterial translocation in an experimental intestinal obstruction model. C-reaclive protein reliability [J] ? Acta Cirurgica Brasileira, 2009, 24(2):98-106.
[5] Wu CC, Lu YZ, Wu LL, et al. Role of myosin light chain kinase in intestinal epithelial barrier defects in a rat model of bowel obstruction[J]. BMC Gastroenterology, 2010, 10:39-51.
[6] 赵静波, Hans Gregersen. 不同疾病动物模型中的胃肠生物力学和组织结构重构[J]. 医学综述, 2008, 14(23): 3553-3557.
[7] Chui CJ, Mcardle AH, Browm R, et al. Intestinal mucosal lesion in low-flow states [J]. Arch Surg,1970,101:478-483.
[8] Yu XY, Zou CL, Zhou ZL, et al. Phasic study of intestinal homeostasis disruption in experiment intestinal obstruction[J].World J Gastroenterol, 2014, 20(25):8130-8138.
[9] 张林. SCL转基因联合SCF促Cajal间质细胞恢复的在体实验研究[D].重庆:第三军医大学, 2007.
[10]Caroline CA, Hennig GW, Kurahashi M, et al. Relationship between interstitial cells of Cajal, fibroblast-like cells and inhibitory motor nerves in the internal anal sphincter[J].Cell Tissue Res, 2011, 344(1):17-30.
[11]Kondo J, Powell AE, Wang Y, et al. LRIG1 regulates ontogeny of smooth muscle-derived subsets of interstitial cells of Cajal in mice[J]. Gastroenterology, 2015, 149(2):407-419.
[12]Zhu MH, Sung TS, O’Driscoll K, et al. Intracellular Ca2+ release from endoplasmic reticulum regulates slow wave currents and pacemaker activity of interstitial cells of Cajal[J]. AM J Physiol Cell Physiol, 2015, 308(8): C608-620.
[13]Sanders KM, Ward SM. Interstitial cells of Cajal: a new perspective on smooth muscle function[J]. J Physiol, 2006, 576(3):721-726.
[14]Torihashi S, Nishi K, Tokutomi Y, et al. Blockade of kit signaling induces transdifferentiation of interstitial cells of Cajal to a smooth muscle phenotype[J]. Gastroenterol, 1999, 117(1):140-148.
[15]Peng MF, Li K, Wang C, et al. Therapeutic effect and mechanism of electroacupuncture at Zusanli on plasticity of interstitial cells of Cajal: a study of rat ilum[J]. BMC Complement Altern Med, 2014,14:186-196.
[16]Gim H, Nam JH, Lee S, et al. Quercetin inhibits pacemaker potentials via nitric oxide/cGMP-dependent activation and TRPM7/ANO1 channels in cultured interstitial cells of Cajal from mouse small intestine[J]. Cell Physiol Biochem, 2015, 35(6):2422-2436.
[17]Gao J, Sathar S, O’Grady G, et al. Developmental changes in postnatal murine intestinal interstitial cell of Cajal network structure and function[J]. Ann Biomed Eng, 2014, 42(8):1729-1739.

丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究

Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	江剑宏, 段仁鹏, 李晓锋. 国人胆道三维重建解剖变异应用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 1-4.
[2]	郭红志, 王瑜, 张加琪, 梁海彬, 马梓玮, 冯伟, 吴忧, 司子燚. 血管铸型结合CT三维模型评估先兆子痫胎盘血管结构[J]. 中国临床解剖学杂志, 2024, 42(1): 5-10.
[3]	李佳伟, 张静, 李灿然, 兰文杰, 籍庆余, 郭志勇, 张云凤, 刘启, 陈清威, 李筱贺. 蒙古族人群股骨近端解剖参数X线测量[J]. 中国临床解剖学杂志, 2024, 42(1): 11-16.
[4]	李琨, 张燕, 郭冉, 郝咪咪, 吴轩宇, 徐艺芳, 王超群, 马文童, 张灵淇, 杨宏宇, 李志军, 张少杰, 王星. 儿童及青少年枕颈角与后枕颈角数字化测量及其临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 17-20.
[5]	李文, 杨思艺, 黄蕾, 卿霁雯, 蒋松涛, 张磊. 基于MRI探讨Lisfranc韧带的形态学特点及临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 21-25.
[6]	李凡凡, 徐阳, 王晓旭. 紫草素调节Nrf2/HO-1信号通路对实验性大鼠肉芽肿性小叶性乳腺炎的治疗作用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 26-32.
[7]	王兴航, 丁佳媛, 李放, 包翠芬, 阎丽菁. 人参皂苷Rg1通过抑制NLRP3炎症小体途径减轻氧糖剥夺/复供后小胶质细胞炎症反应[J]. 中国临床解剖学杂志, 2024, 42(1): 33-41.
[8]	程莹莹, 武建军, 蔡海燕, 焦旭文, 马江波, 丁银秀. 体外炎性诱导星形胶质细胞激活及功能分析[J]. 中国临床解剖学杂志, 2024, 42(1): 42-46.
[9]	祁志, 杨力, 贾秋叶, 陈浩伦, 段兆达, 吴春云. 依达拉奉经MAPKs通路对脂多糖激活的小胶质细胞Sirt3表达调控[J]. 中国临床解剖学杂志, 2024, 42(1): 47-53.
[10]	张晶晶, 王洪新. 黄芩苷通过PDGF/P38 MAPK信号通路对肺动脉高压大鼠的保护作用[J]. 中国临床解剖学杂志, 2024, 42(1): 54-58.
[11]	乐钦, 陈荣丽, 熊婧熙, 王婷怡, 蔡志恒, 易秋实, 曾心怡. 白芨多糖对大鼠跨区皮瓣choke区的影响[J]. 中国临床解剖学杂志, 2024, 42(1): 59-64.
[12]	李笑予, 张磊, 付磊, 李东波, 汪国友. 三步接骨法治疗Sanders Ⅱ型跟骨骨折的有限元研究[J]. 中国临床解剖学杂志, 2024, 42(1): 65-70.
[13]	吴研飞, 马剑雄, 卢斌, 王颖, 柏豪豪, 靳洪震, 马信龙. CT后处理技术重建CT值与终板抗压强度的相关性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 71-76.
[14]	庄万强, 唐毅, 骆勇刚, 张辉. 关节镜联合胫骨高位截骨术对髌骨位置及髌股关节功能影响的早中期回顾性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 77-82.
[15]	罗鹰, 窦伟誉, 吴小杭, 陈璟昆, 彭昌贵, 潘剑英 . 微创海马钢板内固定治疗跟骨骨折并发症的影响因素[J]. 中国临床解剖学杂志, 2024, 42(1): 83-88.