地塞米松对急性肺损伤中TREM-1表达的影响

doi:10.13418/j.issn.1001-165x.2016.05.010

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (5): 523-527.doi: 10.13418/j.issn.1001-165x.2016.05.010

地塞米松对急性肺损伤中TREM-1表达的影响

骆凤¹，　郑浩翔²，　董良³，　李年旺²，　周琴²，　贾哲²，　贺丽萍¹，　孙国瑛^1,4

1.湖南师范大学医学院基础医学系组织学与胚胎学教研室，长沙   410013；    2. 湖南师范大学树达学院
2014级临床医学专业，长沙   410013；    3. 遵义医学院附属医院麻醉科，遵义   563000；
4.中南大学基础医学院，长沙   410006

收稿日期:2016-04-21 出版日期:2016-09-25 发布日期:2016-10-14
通讯作者: 孙国瑛，博士，硕士生导师，E-mail:sunguoying1029@ 163.com
作者简介:骆凤(1992-)，女，湖南岳阳人，学士，在读硕士，主要研究方向：呼吸系统疾病损伤与修复机制研究，Tel:0731-88912441，E-mail:13973161724@163.com
基金资助:
湖南省教育厅科研项目(14C0703)；中南大学博士后基金（160320001）；2015年湖南师范大学树达学院创新课题（012-615）

Effect of dexamethasone on the expression of TREM-1 in acute lung injury

LUO Feng¹, ZHENG Hao-xiang², DONG Liang³, LI Nian-wang², ZHOU Qin², JIA Zhe², HE Li-ping¹, SUN Guo-ying ^1,4

1.Department of Histology and Embryology, Hunan Normal University, Changsha 410013; 2. Clinical Medical Undergraduates of Grade 2014, College of Shu Da, Hunan Normal University, Changsha 410013; 3.Department of Anesthesiology, Affiliated Hospital of Zunyi Medical College, Zunyi 563000;4. College of Basic Medical Sciences, Central South University, Changsha 410006, China

Received:2016-04-21 Online:2016-09-25 Published:2016-10-14

摘要/Abstract

摘要：

目的　探讨地塞米松（Dexamethasone，Dex）对脂多糖（Lipopolysaccharide, LPS）诱导的急性肺损伤(acute lung injury, ALI)小鼠肺组织中髓样细胞表达的触发受体1（triggering receptor expressed on myeloid cells-1,TREM-1）表达的影响。方法　以昆明小鼠为研究对象，腹腔注射LPS(10 mg/kg)建立ALI模型，30 min后给予不同浓度的Dex（5、10、20、40 mg/kg）处理6 h；选用Dex(10 mg/kg)处理不同的时间（6、12、24、36 h）。HE染色法观察肺组织病理损伤程度； RT-PCR检测肺组织TREM-1mRNA的表达；ELISA检测小鼠支气管肺泡灌洗液中可溶性TREM-1（sTREM-1）蛋白水平。结果　Dex可减轻肺病理损伤；Dex呈时间依赖性地下调ALI小鼠肺组织的TREM-1 mRNA的表达，且在6 h即可降低；Dex呈剂量依赖地下调ALI小鼠肺组织中TREM-1 mRNA的表达，并在10 mg/kg时开始降低。Dex可降低ALI小鼠支气管肺泡灌洗液中sTREM-1的蛋白水平。结论　Dex可呈剂量及时间依赖性下调ALI小鼠肺组织中的TREM-1mRNA的表达，并减少肺内髓样细胞胞膜TREM-1的脱落，提示Dex可能通过调节TREM-1的表达，从而抑制ALI早期的炎症级联反应，参与保护肺组织。

关键词: 髓样细胞表达的触发受体-1, 急性肺损伤, 地塞米松, 脂多糖

Abstract:

Objective　To investigate the effects of dexamethasone (Dex) on the TREM-1expression in acute lung injury(ALI) of mice induced by lipopolysaccharide (LPS).　Methods Kunming mice (KM mice) were used as the research object and LPS (10 mg/kg) was used to induce acute lung injury model. After half an hour given different concentrations of Dex (5, 10, 20, 40 mg/kg) and selection of different time points (6, 12, 24 and 36 h) were observed. The lung tissue was observed by HE staining, RT-PCR was used to detect TREM-1 expression, and ELISA was used to detect the expression of sTREM-1 in bronchoalveolar lavage fluid of mice. Results Dex could time-dependently down-regulate the expression of lung tissue in the acute lung injury and in 6h treatment peak; Dexcould dose-dependently down-regulate TREM-1 expression in acute lung injury of lung tissue, and reached the peak at 10 mg /kg. Dex could decrease the protein level of sTREM-1 in bronchoalveolar lavage fluid of ALI mice.　Conclusion　Dex can down-regulate TREM-1 of lung tissues of ALI mice in a dose and time-dependent manner, and reduce myeloid cell membrane of TREM-1 in lung. Our research indicates that Dex may inhibit inflammatory cascade and protect lung tissue by regulation of TREM-1 expression.

Key words: Triggering receptor expressed on myeloid cells-1, Acute lung injury, Dexamethasone;Lipopolysaccharide

骆凤,郑浩翔,董良,李年旺,周琴,贾哲,贺丽萍,孙国瑛. 地塞米松对急性肺损伤中TREM-1表达的影响[J]. 中国临床解剖学杂志, 2016, 34(5): 523-527.

LUO Feng, ZHENG Hao-xiang, DONG Liang, LI Nian-wang, ZHOU Qin, JIA Zhe, HE Li-ping,SUN Guo-ying. Effect of dexamethasone on the expression of TREM-1 in acute lung injury[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 523-527.

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地塞米松对急性肺损伤中TREM-1表达的影响

Effect of dexamethasone on the expression of TREM-1 in acute lung injury

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