BMP-2转染BMSCs复合镁合金棒修复兔股骨头坏死的实验研究

doi:10.13418/j.issn.1001-165x.2016.05.011

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (5): 528-533.doi: 10.13418/j.issn.1001-165x.2016.05.011

BMP-2转染BMSCs复合镁合金棒修复兔股骨头坏死的实验研究

张雁儒^1,2，　张辉³，　卡卡¹，　张戈宸³，　尚艳⁴，　孙昊³，　李肖肖⁴，　黄文华²

1.河南理工大学医学院，河南焦作 454000；　2.南方医科大学临床解剖学研究所, 广州 510515；
3.郑州大学第一附属医学院骨科，郑州 450052； 4.郑州市儿童医院，郑州 450062

收稿日期:2015-12-23 出版日期:2016-09-25 发布日期:2016-10-14
通讯作者: 黄文华，教授，博士生导师，E-mail: 13822232749@139.com
作者简介:张雁儒（1970-），男，河南西华人，教授，博士，主要从事股骨头坏死及临床解剖学方面的研究，Tel：（0391）3986833，E-mail：jiaoxueban2010@126.com

BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis

ZHANG Yan-ru ^1,2, ZHANG Hui³, Kaka¹, ZHANG Ge-chen³, SHANG Yan⁴, SUN Hao³, HUANG Wen-hua²

1. Medical School of Henan Polytechnic University, Jiaozhuo，Henan 454000; 2. Institute of Clinical Anatomy, Southern Medical University, Guangzhou 510515; 3. Department of Orthopedic of First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052; 4. Department of laboratory of Zhengzhou City Children's Hospital, Zhengzhou 450062, China

Received:2015-12-23 Online:2016-09-25 Published:2016-10-14

摘要/Abstract

摘要：

目的　探讨BMP-2转染BMSCs复合镁合金棒修复兔股骨头坏死的的效果。方法　取一只兔分离、培养兔BMSCs，采用电转法将成功转染pcDNA3.1-BMP-2的质粒导入BMSCs。取40只兔子用液氮冷冻法制造股骨头坏死模型，然后随机分为4组，每组10只，分别为镁棒/BMSCs组，镁棒组，BMSCs组，空白组。镁棒组和镁棒/BMSCs组是指利用生物钻将生物镁合金棒置入坏死股骨头，同时镁棒/BMSCs组和BMSCs组导入转染pcDNA3.1-BMP-2质粒的BMSCs。术后6周和12周分别取股骨头标本观察修复效果。结果　术后第1、2、4、8、12周分别检验各组兔血液、尿液中镁离子浓度。第6、12周X线、HE染色显示，镁棒/BMSCs组股骨头恢复最好，第12周镁合金棒基本完全吸收；镁棒组和BMSCs组股骨头坏死进展较慢，部分组股骨头坏死有好转；空白组股骨头坏死加重。结论　植入镁合金棒联合BMP-2基因复合BMSCs有延缓和修复股骨头缺血性坏死的作用。

关键词: BMP-2, BMSCs, 镁合金, 股骨头坏死

Abstract:

Objective 　To investigate the effect of BMSCs transfected BMP-2 composite with magnesium alloy rod in the repair of the femoral head necrosis. Methods Rabbit BMSCs was isolated, cultured, and successfully transfected pcDNA3.1-BMP-2 plasmid by electroporation. 40 rabbit femoral head necrosis was established by liquid nitrogen freezing method, and then were randomly divided into 4 groups of 10 animals each: the magnesium rod /BMSCs group, the magnesium rod group, the BMSCs group, and the blank group. The bio-magnesium alloy rod imported the BMSCs-BMP-2 plasmid pcDNA3.1. Repair effect was assessed post-operatively at 6 weeks and 12 weeks.　Results　Six weeks and 12 weeks after operation, the concentration of magnesium ion in blood and urine was tested by first, two, four, eight and twelve weeks, respectively. X-ray and HE staining at the sixth and twelfth week showed that the femoral head of magnesium rod /BMSCs group had the best recovery, and at the twelfth week, the magnesium alloy rod was completely absorbed; the femoral head necrosis in the magnesium rod group and BMSCs group was slower, and some of the femoral head necrosis was improved.　Conclusion　Implantation of composite magnesium alloy-BMSCs-BMP-2 gene has effect on delaying femoral head necrosis and improving repair.

Key words: BMSCs, BMP-2, Magnesium alloy, Femoral head necrosis, Rabbits

张雁儒,张辉,卡卡,张戈宸,尚艳,孙昊,李肖肖,黄文华. BMP-2转染BMSCs复合镁合金棒修复兔股骨头坏死的实验研究[J]. 中国临床解剖学杂志, 2016, 34(5): 528-533.

ZHANG Yan-ru,ZHANG Hui, Kaka, ZHANG Ge-chen, SHANG Yan, SUN Hao, HUANG Wen-hua. BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 528-533.

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BMP-2转染BMSCs复合镁合金棒修复兔股骨头坏死的实验研究

BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis

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