脑缺血再灌注损伤程度与自噬相关基因LC3、mTOR表达的相关性研究

doi:10.13418/j.issn.1001-165x.2018.02.012

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (2): 182-186.doi: 10.13418/j.issn.1001-165x.2018.02.012

脑缺血再灌注损伤程度与自噬相关基因LC3、mTOR表达的相关性研究

李丹丹¹，　肖学进¹，　秦书俭²，　包翠芬³

锦州医科大学　1. 细胞生物学教研室， 2. 解剖学教研室， 3. 基础医学实验教学中心，辽宁锦州 121001

出版日期:2018-03-25 发布日期:2018-05-04
通讯作者: 包翠芬，教授，硕士生导师，E-mail：mianyizuhua　@aliyun.com
作者简介:李丹丹（1990-），硕士，主要研究方向为脑缺血再灌注损伤的防治研究，Tel：13134161884，E-mail：397929461@qq.com
基金资助:
国家自然基金（81774116）；辽宁省科技厅计划项目（20170540378，2015020696）；辽宁省教育厅一般项目（L2015324）

Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR

LI Dan-dan¹， XIAO Xue-jin¹， QIN Shu-jian²， BAO Cui-fen³

1. Department of Cell Biology，2. Department of Anatomy，3. Basic Medical Experimental Teaching Center,Jinzhou Medical University, Jinzhou 121001, Liaoning Province, China

Online:2018-03-25 Published:2018-05-04

摘要/Abstract

摘要：

目的　探讨局灶性脑缺血再灌注损伤（cerebral ischemia reperfusion, CIR）程度与自噬微管相关蛋白轻链3抗体-Ⅱ（Autophagy microtubule associated protein light chain 3 antibody-Ⅱ，LC3-Ⅱ）、雷帕霉素靶蛋白抗体（Rapamycin target protein antibody，mTOR）表达的相关性。方法　采用线栓阻断法阻塞大脑中动脉2 h制作大鼠局灶性脑缺血再灌注模型；观察各组间的神经功能缺失评分、TTC梗死体积；应用免疫印迹、免疫荧光法检测大脑顶叶的LC3-Ⅱ、mTOR的表达。结果　TTC染色显示，梗死体积在缺血再灌24 h达高峰。WB、免疫荧光显示，LC3-Ⅱ在IR中表达增加，且在缺血再灌24 h表达最高；mTOR在缺血再灌24 h、48 h表达最低。相关性分析显示，TTC染色梗死体积与LC3-Ⅱ表达水平高度正相关。结论　脑缺血再灌注损伤程度可能与LC3、mTOR的表达水平相关。

关键词: 自噬, 　局灶性脑缺血再灌注, 　自噬微管相关蛋白轻链3抗体, 　雷帕霉素靶蛋白抗体

Abstract:

Objective　To investigate the correlation between the degree of focal cerebral ischemia-reperfusion injury and the expression of LC3-Ⅱ and mTOR.　Methods　The models of focal cerebral ischemia-reperfusion in rats were established by occlusion of the middle cerebral artery. Neurological deficit score and TTC infarct volume were observed among groups. The expressions of LC3-Ⅱ and mTOR in different groups were detected by immunoblotting and immunofluorescence. Results　Immunoblotting and immunofluorescence showed that LC3-Ⅱ had the highest expression in 24 hours after ischemia and reperfusion (IR 24 h), and mTOR was the lowest in IR 24h and IR 48 h. Correlation analysis showed that the infarcted volume of TTC was positively correlated with the expression level of LC3-II.　Conclusion 　IR may activate the autophagy of the parietal neurons of the brain by increasing the expression level of LC3-II and decreasing the expression level of mTOR. The degree of cerebral ischemia-reperfusion injury may be related to the expression level of LC3 and mTOR.

Key words: Autophagy, 　Cerebral ischemia reperfusion, 　Autophagy microtubule associated protein light chain 3 antibody-Ⅱ, 　Rapamycin target protein antibody

李丹丹,肖学进,秦书俭,包翠芬. 脑缺血再灌注损伤程度与自噬相关基因LC3、mTOR表达的相关性研究[J]. 中国临床解剖学杂志, 2018, 36(2): 182-186.

LI Dan-dan,XIAO Xue-jin,QIN Shu-jian,BAO Cui-fen. Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(2): 182-186.

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脑缺血再灌注损伤程度与自噬相关基因LC3、mTOR表达的相关性研究

Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR

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