白藜芦醇治疗POI大鼠药物浓度筛选

doi:10.13418/j.issn.1001-165x.2018.02.017

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (2): 206-210.doi: 10.13418/j.issn.1001-165x.2018.02.017

白藜芦醇治疗POI大鼠药物浓度筛选

李宁，刘丽丽

锦州医科大学附属第一医院妇产科，锦州 121000

收稿日期:2017-12-05 出版日期:2018-03-25 发布日期:2018-05-04
通讯作者: 刘丽丽，教授，硕士生导师，E-mail： clearsky315@163.com
作者简介:李宁（1992-），在读硕士，主要研究方向为生殖内分泌，Tel：13704168662, E-mail：lining8662@163.com

Screening of drug concentration of resveratrol in POI rats

LI Ning , LIU Li-li

First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, Liaoning Province, China

Received:2017-12-05 Online:2018-03-25 Published:2018-05-04

摘要/Abstract

摘要：

目的　通过观察不同浓度白藜芦醇治疗早发性卵巢功能不全（POI）成模大鼠的疗效，确定其治疗早发性卵巢功能不全大鼠的最佳药物浓度。方法　将90只5周龄SPF级SD健康雌性大鼠按随机数字表分为低剂量白藜芦醇灌胃治疗组(a组18只)、中剂量白藜芦醇灌胃治疗组(b组18只)、高剂量白藜芦醇灌胃治疗组(c组18只)、模型对照组(d组18只)、空白对照组(e组18只)。e组从造模前的动物中随机抽取；a、b、c、d组从成功造模（72只给予雷公藤多苷片50 mg·kg-1·d-1 灌胃14 d，根据观测指标确定早发性卵巢功能不全成模）的动物中随机分组获得。a、b、c组分别采用每日20、50、80 mg/kg 灌胃剂量，模型对照组采用0.5％羧甲基纤维素钠溶液灌胃。42 d后采用ELISA法检测大鼠血清性激素：抗苗勒管激素（AMH）、黄体生成素（LH）、卵泡刺激素（FSH）、雌二醇（E2）水平，HE染色镜检大鼠单位卵巢面积内成熟卵泡、闭锁卵泡和黄体数，评价其疗效。结果　不同浓度白藜芦醇灌胃治疗组POI大鼠较0.5％羧甲基纤维素溶液灌胃组POI大鼠血清中AMH、E2水平高（P<0.05），LH、FSH水平低（P<0.05）。不同浓度白藜芦醇灌胃治疗组POI大鼠较空白对照组大鼠血清中AMH、E2水平低（P<0.05），LH、FSH水平高（P<0.05）。与a、b、c、e组比较，d组卵巢内颗粒细胞层数，成熟卵泡数以及黄体数量少（P<0.05），闭锁卵泡数明显增加（P<0.05）。结论　不同浓度白藜芦醇灌胃治疗POI大鼠均有一定的疗效，50 mg/kg浓度效果最好，是最佳药物浓度。

关键词: 早发性卵巢功能不全, 　白藜芦醇, 　最佳药物浓度

Abstract:

Objective　To observe the curative effect of different concentrations of resveratrol on rats with premature ovarian insufficiency (POI) and to determine the optimal drug concentration of resveratrol for treating premature ovarian insufficiency dysfunction in rats.　Methods　A total of 90 5-week-old SPF grade SD healthy female rats were randomly divided into a low-dose resveratrol group (18 rats in group a) and a middle-dose resveratrol group (18 rats in group b), a high dose resveratrol group (18 rats in group c), a model group (18 rats in group d) and a blank control group (18 rats in group e). Group a, group b, group c were administered with a daily dose of 20 mg/kg, 50 mg/kg, 80 mg/kg by gavage, respectively; the model control group was administered with 0.5% sodium carboxymethyl cellulose solution by gavage. ELISA was used to detect the level of serum sex hormone levels: anti-Mullerian hormone (AMH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2) level and HE staining of rats to observe unit ovarian area of mature follicles, atresia follicles and corpus luteum, evaluation of resveratrol efficacy.　Results　Rats treated with different concentration of resveratrol had a significantly increased level of serum AMH and E2 compared to rats treated with 0.5% carboxymethyl cellulose solution (P<0.05), LH and FSH levels were lower (P<0.05).The serum level of AMH and E2 in POI rats treated with resveratrol at different concentration were lower (P<0.05) and LH and FSH were higher (P<0.05) than those of rats in the blank control. Compared with rats in group a, b, c and e, the number of granulosa cells, mature follicles and corpus luteum in ovaries was smaller in group d (P<0.05); In contrast, the number of atresia follicles was significantly increased in group d(P<0.05). Conclusion　Intragastric treatment with different concentration of resveratrol of all have a certain effect on POI rats receiving, with 50 mg/kg showing the best result, and thus being the best drug concentration.

Key words: Premature ovarian insufficiency, 　Resveratrol, 　Optimal Drug concentration

李宁,刘丽丽. 白藜芦醇治疗POI大鼠药物浓度筛选[J]. 中国临床解剖学杂志, 2018, 36(2): 206-210.

LI Ning,LIU Li-li. Screening of drug concentration of resveratrol in POI rats[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(2): 206-210.

参考文献

[1] 中华医学会妇产科学分会绝经学组. 早发性卵巢功能不全的激素补充治疗专家共识[J]. 中华妇产科杂志, 2016, 51(12): 881-886.
[2] de Vet A, Laven JS, de Jong FH, et al. Antimüllerian hormone serum levels: a putative marker for ovarian aging[J]. Fertil Steril, 2002, 77(2): 357-362.
[3] La Marca A, Broekmans FJ, Volpe A, et al. AntiMullerian hormone (AMH): What do we still need to know[J]? Hum Reprod, 2009, 24(9): 22642275.
[4] Anderson RA. What does antiMüllerian hormone tell you about ovarian function[J]? Clin Endocrinol (Oxf), 2012, 77(5): 652655.
[5] Karkanaki A, Vosnakis C, Panidis D. The clinical significance of anti-Mullerian hormone evaluation in gynecological endocrinology[J]. Hormones ( Athens), 2011, 10(2): 95-103．
[6] Lie Fong S, Lugtenburg PJ, Schipper I, et al. AntiMüllerian hormone as a marker of ovarian function in women after chemotherapy and radiotherapy for haematological malignancies[J]. Hum Reprod, 2008, 23(3): 674678.
[7] Xia T, Fu Y, Gao H, et al. Recovery of ovary function impaired by chemotherapy using Chinese herbal medicine in a rat model[J]. Syst Biol Reprod Med, 2014, 60(5): 293303.
[8] Said RS, ElDemerdash E, Nada AS, et al. Resveratrol inhibits inflammatory signaling implicated in ionizing radiationinduced premature ovarian failure through antagonistic crosstalk between silencing information regulator 1 (SIRT1) and poly (ADPribose) polymerase 1 (PARP1) [J]. Biochem Pharmacol, 2016, 103: 140150.
[9] MolgaardHansen L, Skou AS, Juul A, et al. Pubertal development and fertility in survivors of childhood acute myeloid leukemia treated with chemotherapy only: a NOPHOAML study[J]. Pediatr Blood Cancer, 2013, 60(12): 19881995.
[10]Baur JA, Sinclair DA. The rapeutic potential of resveratrol:The in vivo evidence [J]. Nat Rev Drug Discov, 2006, 5(6): 493-506.
[11]曾又佳, 李顺民. 白藜芦醇拮抗雷公藤甲素卵巢毒性实验研究[J]. 新中医, 2014, 46(8): 172-174.
[12]Kong XX, Fu YC, Xu JJ, et al. Resveratrol, an effective regulator of ovarian development and oocyte apoptosis [J]. J Endocrinol Invest, 2011, 34 (11): 374-381.
[13]郝娟, 王春莲, 王培嵩, 等. 雷公藤多甙片致卵巢早衰大鼠动物模型的研究[J]. 实用妇产科杂志, 2012, 27(12): 1866-1870.
[14]黄辉屠, 润琪, 刘芳丽, 等. 白藜芦醇对慢性铅暴露小鼠脑组织Nrf2 信号通路的影响[J]. 卫生研究, 2015, 44(6): 954-958.
[15]Fu Y, Zhao Z, Wu Y, et al. Therapeutic mechanisms of Tongmai Dasheng Tablet on tripterygium glycosides induced rat model for premature ovarian failure[J]. Journal of ethnopharmacol, 2012, 139(1): 26-33.
[16] Özcan P, F?ç?c?o?lu C, Y?ld?r?m ÖK, et al. Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague-Dawley rats [J]. Reprod Biomed Online, 2015, 31(3): 404-410.
[17]刘长江, 毕文超, 张晨, 等. 白藜芦醇对大鼠脊髓损伤后炎症反应的影响[J]. 西安交通大学学报（医学版）, 2013, 34(6): 779-784.

白藜芦醇治疗POI大鼠药物浓度筛选

Screening of drug concentration of resveratrol in POI rats

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

Metrics

本文评价

推荐阅读 0

[1]	米晓璐, 李百艳, 李雅琴, 朱保月, 王维展. 白藜芦醇对腔隙性脑梗死大鼠脑损伤的影响[J]. 中国临床解剖学杂志, 2022, 40(6): 683-688.
[2]	冯成军, 周艳萌, 田应娟. 基于Nrf2/ARE信号通路探讨白藜芦醇治疗非酒精性脂肪性肝炎大鼠的作用机制[J]. 中国临床解剖学杂志, 2021, 39(5): 579-585.