冻融循环及物理匀浆制备高质量脂肪脱细胞基质

doi:10.13418/j.issn.1001-165x.2018.04.008

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (4): 392-396.doi: 10.13418/j.issn.1001-165x.2018.04.008

冻融循环及物理匀浆制备高质量脂肪脱细胞基质

彭章松，　何云帆，　鲁峰

南方医科大学南方医院整形外科，广州 510515

收稿日期:2018-05-22 出版日期:2018-07-25 发布日期:2018-08-21
通讯作者: 鲁峰, 教授，主任医师，E-mail: doctorlufeng@hotmail.com
作者简介:共同第一作者：彭章松(1988-)，研究方向：组织工程及再生医学；何云帆(1992-)，研究方向：组织工程及再生医学，Tel：(020)61641869
基金资助:
国家自然科学基金（81471881，81372083，81171834），广州市健康医疗协同创新重大专项（7414275040815），广东省自然科学基金（S2012010009370），广州市科技计划重点项目（11C32120716）

Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization

PENG Zhang-song, HE Yun-fan, LU Feng

Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China

Received:2018-05-22 Online:2018-07-25 Published:2018-08-21

摘要/Abstract

摘要：

目的　采用冻融循环及物理匀浆制备脂肪脱细胞基质（Decellularized adipose tissue, DAT）以提高其自发诱导成脂能力。方法　利用匀浆法在短时间内对脂肪组织进行脱油，再经后续脱细胞处理后制备高质量DAT（DAT-1），传统方式制备的DAT（DAT-2）作为对照。检测基质相关蛋白CollagenⅣ和Laminin的含量，并植入小鼠体内验证脂肪再生效果。结果　DAT-1实现了有效的脱细胞处理，且DAT-1较DAT-2有更多基质关键性蛋白CollagenⅣ，Laminin的保留，小鼠体内移植结果进一步表明DAT-1比DAT-2具有更好脂肪再生能力。结论　物理匀浆是一种快速高效地去油方式，避免化学试剂对细胞外基质的破坏，提高DAT支架上关键性蛋白的保留，促进了DAT体内脂肪再生。

关键词: , 脱细胞基质, 　冻融循环, 　物理匀浆, 　脂肪再生

Abstract:

Objective　Decellularized adipose tissue (DAT) was prepared by physical homogenization to improve its ability of spontaneous lipid induction.　Methods　In this study, we used homogenization method to remove lipid from adipose tissue, a novel DAT (DAT-1) was fabricated after subsequent decellularization process. Ordinary DAT served as control.　Results　The results revealed that DAT-1 achieved the same decellularization efficiency, but exhibited better retention of ECM components (laminin and collagen Ⅳ) and in vivo recellularization, compared to those with ordinary DAT.　Conclusions 　Mechanical homogenization is a rapid and efficient method to remove lipid from adipose tissue, which could avoid the use of harsh chemical agent and lead to the better retention of ECM components, promoting the in vivo adipose tissue regeneration of DAT.

Key words: Decellularized matrix, 　Freeze-thaw cycle, 　Mechanical homogenization, 　Adipose tissue regeneration

彭章松，　何云帆，　鲁峰. 冻融循环及物理匀浆制备高质量脂肪脱细胞基质[J]. 中国临床解剖学杂志, 2018, 36(4): 392-396.

PENG Zhang-song, HE Yun-fan, LU Feng. Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(4): 392-396.

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冻融循环及物理匀浆制备高质量脂肪脱细胞基质

Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization

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