关键词: 缺血, 　缺氧, 　自噬, 　低氧诱导因子, 　心肌损伤

Abstract:

Objective　To investigate the effect of HIF-1α on autophage-associated genes Beclin1, LC3I and LC3II, and the regulation on autophagy.   Methods　The model of oxygen-glucose deprivation (OGD) was established, and western blot was used to detect interference effect of HIF-1α siRNA. The CCK-8 assay was used to detect the effect of HIF-1α siRNA on the activity of myocardial cells in OGD model. The effect of HIF-1α siRNA on autophagy-associated genes Beclin1, LC3I and LC3II expression was detected by RT-qPCR and western blot.    Results   The results of western blot showed that HIF-1α siRNA could effectively knock down the expression of HIF-1α in myocardial cells in OGD model. The result of CCK-8 assay showed that HIF-1α siRNA could reduce the activity of myocardial cells in OGD model. RT-qPCR and western blot results showed that HIF-1α siRNA could reduce the expression of the autophagy-associated genes Beclin1, LC3I and LC3II, and reduce the ratio of LC3II to LC3I at mRNA and protein level.  Conclusion    HIF-1α siRNA could knock down the expression of HIF-1α in the OGD model, resulting in further decrease of the activity of myocardial cells，with the Beclin1 expression and ratio of LC3II/LC3I reduced. HIF-1α plays a role of myocardial protection in the OGD model by regulation of autophagy.

Key words:  Ischemia, 　Hypoxia, 　Autophagy;　Hypoxic induction factor, 　Myocardial injury