神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究

doi:10.13418/j.issn.1001-165x.2018.06.005

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (6): 621-625.doi: 10.13418/j.issn.1001-165x.2018.06.005

神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究

韩建龙¹，　邱思强¹，　朱新炜¹，　栾方海¹，　常刚¹，　程继祖²，　左金良¹

1.泰山医学院济南临床学院/济南市第四人民医院，济南 250031； 2.济南市桑梓医院，济南 250000

收稿日期:2018-06-07 出版日期:2018-11-25 发布日期:2018-12-29
通讯作者: 左金良，主任医师，E-mail: zjl.md@163.com
作者简介:韩建龙（1979-），副主任医师，研究方向：脊柱疾病，Tel: 15589917693, E-mail: hjlty@163.com
基金资助:
国家自然基金项目（81472138）

The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit

HAN Jian-long¹, QIU Si-qiang¹, ZHU Xin-wei¹, LUAN Fang-hai¹, CHANG Gang¹, CHENG Ji-zu², ZUO Jin-liang¹

1. Jinan Clinical College of Taishan Medical University /Department of Orthopedics, The 4th Hospital of Jinan, Jinan 250031, China; 2. Department of Orthopedics, Sangzi Hospital of Jinan, Jinan 250031, China

Received:2018-06-07 Online:2018-11-25 Published:2018-12-29

摘要/Abstract

摘要：

目的　通过电刺激兔颈脊神经节，检测颈交感神经节内的神经肽Y （neuropeptide Y，NPY）含量，研究颈交感神经节与颈脊神经节间的通路联系，探讨颈性眩晕发病机制中的神经解剖学基础。方法　96只新西兰兔随机分为C2、C3、C4、C5、C6、C7组及相应对照组（每组8只），麻醉后显露相应的脊神经节，给予波宽0.5 ms、频率30 Hz、5 V电刺激，5秒/次，每1 min重复1次，共5次，30 min后，灌注固定，切取双侧颈上、下交感神经节，切片，免疫反应，染色，观察。结果　电刺激C2、C3脊神经节后，同侧颈上交感神经节内NPY含量升高（P<0.05）；电刺激C4、C5脊神经节后，同侧颈上交感神经节和颈下交感神经节内NPY含量均有明显变化（P<0.05）；电刺激C6、C7脊神经节后，同侧颈下交感神经节内NPY含量升高（P<0.05）。结论　颈脊神经节和颈交感神经节间存在神经电生理上的通路联系，且具有节段性、同侧性特点，为颈性眩晕的发病机制及临床分型提供了实验依据和理论基础。

关键词: 颈脊神经节, 　颈交感神经节, 　神经肽Y（NPY）, 　颈性眩晕, 　神经反射弧

Abstract:

Objective　To study the neural pathway between cervical spinal ganglia and cervical sympathetic ganglia, and to explore the neural factors in the pathogenesis of cervical vertigo by detecting the neuropeptide Y (NPY) in cervical sympathetic ganglia after the cervical spinal ganglia electro-stimulated. Methods　96 New Zealand rabbits were divided into C2, C3, C4, C5, C6, C7 and control groups randomly (n=8 in each group ). The electro-stimulations (width=0.5 ms, 30 Hz, 5 V, time=5 s, 1 time/1 min, and 5 times total) were given to specific spinal ganglia which were revealed. After 30 min, the rabbits were killed through perfusion and the superior cervical sympathetic ganglia (SCG) and inferior cervical sympathetic ganglia (ICG) of both sides were cut. Then the section were made after immunologic reaction and observed under the microscope.　Results　The content of NPY in the same side sympathetic ganglia was higher than the other side sympathetic ganglia and the control group sympathetic ganglia after the electro-stimulation was given to the cervical spinal ganglia, and the difference was statistic significance (P<0.05). When the electro-stimulation was given to the C2, C3 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in SCG mainly; when the electro-stimulation were given to the C4, C5 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in SCG and ICG; when the electro-stimulation were given to the C6, C7 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in ICG mainly. Conclusion　There is some kind of neural pathway between the cervical spinal ganglia and cervical sympathetic ganglia, and the characteristic of segment and homonymy in this kind of connection is also found, which provides a neuroanatomical basis for the hypothesis of reflex arc in cervical vertigo.

Key words: Cervical spinal ganglion, 　Cervical ganglion, 　Neuropeptide Y, 　Cervical vertigo, 　Nervous reflex arc

韩建龙,邱思强,朱新炜,栾方海,常刚,程继祖,左金良. 神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究[J]. 中国临床解剖学杂志, 2018, 36(6): 621-625.

HAN Jian-long, QIU Si-qiang, ZHU Xin-wei, LUAN Fang-hai, CHANG Gang, CHENG Ji-zu, ZUO Jin-liang. The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(6): 621-625.

参考文献

[1] Li Y, Peng B. Pathogenesis, diagnosis, and treatment of cervical vertigo[J]. Pain Physician, 2015, 18(4): E583-E595.
[2] 左金良, 邱思强, 魏光伟, 等. 逆行标记法研究颈脊神经节至交感神经节的神经反射通路[J]. 解剖学报, 2011, 42(5): 615-618.
[3] Zuo J, Han J, Qiu S, et al. Neural reflex pathway between cervical spinal and sympathetic ganglia in rabbits: implication for pathogenesis of cervical vertigo[J]. Spine J, 2014, 14(6): 1005-1009.
[4] Zuo J, Han J, Qiu S, et al. Nerve fiber connection between the cervical spinal ganglia and cervical sympathetic ganglia: an in vivo anatomic study in rabbits[J]. J Hard Tissue Biology, 2013, 22(2): 211-214.
[5] Yacovino DA, Hain TC. Clinical characteristics of cervicogenic-related dizziness and vertigo[J]. Semin Neurol, 2013, 33(3): 244-255.
[6] Perlmutter DH, Petraglia AL, Barbano R, et al. Microvascular decompression in patient with atypical features of hemifacial spasm secondary to compression by a tortuous vertebrobasilar system: case report[J]. Neurosurgery, 2010, 66(6): E1212.
[7] Ongun N, Atalay NS, Degirmenci E, et al. Tetra-ataxiometric posturography in patients with migrainous vertigo[J]. Pain Physician, 2016, 19(1): E87-E95.
[8] Johnston JL, Daye PM, Thomson GT. Inaccurate saccades and enhanced vestibulo-ocular reflex suppression during combined eye-head movements in patients with chronic neck pain: possible implications for cervical vertigo [J]. Front Neurol, 2017, 8: 23.
[9] van Leeuwen RB, van der Zaag-Loonen H. Dizziness and neck pain: a correct diagnosis is required before consulting a physiotherapist [J]. Acta Neurol Belg, 2017, 117(1): 241-244.
[10] Al Saif AA, Al Senany S. Determine the effect of neck muscle fatigue on dynamic visual acuity in healthy young adults. J Phys Ther Sci, 2015, 27(1): 259-263.
[11] Dieterich M. Dizziness[J]. Neurologist, 2004, 10(3): 154-164.
[12] Lundberg JM, Tatemoto K. Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade[J]. Acta Physiol Scand, 1982, 116(4): 393-402.
[13] Galli S, Naranjo A, Van Ryn C, et al. Neuropeptide Y as a biomarker and therapeutic target for neuroblastoma[J]. Am J Pathol, 2016, 186(11): 3040-3053.
[14] 康永生, 高延征. 颈性眩晕与神经肽Y相关性研究[J]. 中华实用诊断与治疗杂志, 2011(11): 1041-1042.

神经肽Y在兔颈部交感神经节与脊神经节间通路应用研究

The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

Metrics

本文评价

推荐阅读 0