中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (6): 632-636.doi: 10.13418/j.issn.1001-165x.2018.06.007

• 实验研究 • 上一篇    下一篇

鼠李素下调Notch-1的表达对乳腺癌MCF-7细胞增殖、侵袭和迁移的调节作用

张淑艳1, 张方1, 姬颖华2   

  1. 1.鹤壁职业技术学院护理学院,  河南   鹤壁    458030; 2.新乡医学院第一附属医院,  河南   卫辉    453100
  • 收稿日期:2018-04-25 出版日期:2018-11-25 发布日期:2018-12-29
  • 通讯作者: 张淑艳(1980-),硕士,讲师,研究方向:临床医学,Tel:13193470852,E-mail:liangyongcds@sina.com
  • 作者简介:张淑艳(1980-),硕士,讲师,研究方向:临床医学,Tel:13193470852,E-mail:liangyongcds@sina.com
  • 基金资助:

    河南省医学科技公关计划项目(201203068)

Rhamnetin regulates the proliferation, invasion and migration of breast cancer MCF-7 via down-regulating the expression of Notch-1

ZHANG Shu-yan1, ZHANG Fang1, JI Ying-hua2   

  1. 1.College of nursing, Hebi College of Vocation and Technology, Hebi 458030, Henan Province, China;  2. First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China
  • Received:2018-04-25 Online:2018-11-25 Published:2018-12-29

摘要:

目的 探索鼠李素对乳腺癌MCF-7细胞增殖,侵袭和迁移的影响及其分子机制。  方法 MCF-7细胞分为4组:MCF-7(对照组)、鼠李素(1、2.5、5 μM)组。CCK-8检测细胞增殖。Transwell检测细胞侵袭。划痕实验分析细胞迁移。蛋白印记检测Notch-1、CSL、Hes1、Ki67和VEGF表达。  结果 低浓度的(< 5 μM)的鼠李素不会影响MCF-7细胞活力;高浓度的(> 5 μM)的鼠李素对MCF-7有显著毒性。2.5和5 μM鼠李素组细胞增殖倍数明显低于对照组(P<0.05)。与对照组相比,鼠李素(1、2.5、5 μM)组细胞侵袭和迁移明显降低(P <0.05)。另外,鼠李素(1、2.5、5 μM)组Notch-1、CSL和Hes1相对蛋白表达量都明显少于对照组(P < 0.05)。而且,Notch-1通路激活剂Jagged1(10 μM)还可明显抑制鼠李素(5 μM)诱导的Notch-1、CSL、Hes1、Ki67和VEGF表达的下降(P < 0.05)。  结论 鼠李素通过下调Notch-1的表达抑制乳腺癌MCF-7细胞增殖,侵袭和迁移。

关键词: 乳腺癌,  鼠李素,  增殖,  侵袭,  迁移,  Notch-1通路

Abstract:

Objective To explore the effect, and its molecular mechanism, of rhamnetin on cell proliferation, invasion and migration of MCF-7 cell which is a kind of breast cancer cell.   Methods MCF-7 cells were divided into four groups: MCF-7 (control group) and rhamnetin (1, 2.5, 5 μM) groups. Cell proliferaion was detected by CCK-8. Cell invasion was measured by transwell. Cell migration was tested by wound healing. The expression of Notch-1, CSL, Hes1, Ki67 and VEGF were detected by western blot.   Results    Low concentration (< 5 μM) rhamnetin had no effect on the cell viability of MCF-7, while it was quite opposite under the circumstance of high concentration (> 5 μM) rhamnetin. The proliferation in rhamnetin (2.5, 5 μM) groups were lower than that of control group (P<0.05). Compared with control group, the invasion and migration in rhamnetin (1, 2.5, 5 μM) groups were decreased (P<0.05) with lower expression of Notch-1, CSL and Hes1 in rhamnetin (P<0.05). Moreover, the rhamnetin (5 μM)-induced declined expression of Notch-1, CSL, Hes1, Ki67 and VEGF were repressed by Notch-1 pathway activating agent Jagged1 (10 μM).   Conclusions Rhamnetin can restrain the proliferation, invasion and migration of MCF-7 via down-regulating the expression of Notch-1.

Key words: Breast cancer;   , Rhamnetin;   , Proliferation;   , Invasion;   , Migration;   , Notch-1 pathway