Objective To explore the anatomical structure of fetal gastric artery and its clinical application. Methods Gross specimens of fetal gastric arteries was perfused and measured. The data of CT scanning was introduced to reconstruct the three-dimensional fetal gastric artery and segment the blood vessels in Mimics 19.0 software. The results were compared with the adult gastric artery. Results The fetal gastric artery perfusion specimens showed clearly the distribution and anastomoses of the arteries in the fetal stomach. The biofidelity and the third dimension of three-dimensional visualized model were high and strong. The main branches of the arteries in the fetal stomach, the distribution and shape of fetal gastric arteries could be observed by model segmentation. Conclusion The blood supply of fetal surgery is evidently different from that of adult gastric artery, which provides anatomical basis for fetal intrauterine surgery and basic research of fetal medicine.