miR-143-3p 靶向MAPK1对人结肠癌细胞增殖，凋亡和侵袭的调节作用#br#

doi:10.13418/j.issn.1001-165x.2019.03.011

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (3): 292-298.doi: 10.13418/j.issn.1001-165x.2019.03.011

miR-143-3p 靶向MAPK1对人结肠癌细胞增殖，凋亡和侵袭的调节作用#br#

张志谦¹，　张广星²，　彭小东²

1.南昌市第三医院普外科，南昌 330009； 2.南昌大学第一附属医院肿瘤内科，南昌 330006

收稿日期:2018-09-26 出版日期:2019-05-25 发布日期:2019-06-13
通讯作者: 彭小东，主任医师，硕士生导师，E-mail: pxddhbb@163.com
作者简介:张志谦（1971-），副主任医师，学士，主要从事普通外科临床工作及基础理论方面的研究，E-mail: 2240706571@qq.com
基金资助:
江西省科技厅支持项目(20152BBG70054)

The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1

ZHANG Zhi-qian¹, ZHANG Guang-xin², PENG Xiao-dong²

1. Department of General Surgery, The Third Hospital of Nanchang，Nanchang 330009, China; 2. Department of Medicine Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China

Received:2018-09-26 Online:2019-05-25 Published:2019-06-13

摘要/Abstract

摘要： 目的　研究miR-143-3p靶向MAPK1与人结肠癌细胞增殖、凋亡和侵袭的关系。方法　qRT-PCR检测miR-143-3p mimic转染效果和MAPK1的mRNA表达水平；双荧光素酶报告实验分析miR-143-3p与MAPK1的靶向关系；蛋白质印迹检测MAPK1的蛋白表达水平，CCK-8检测细胞增殖倍数，Hoechst染色检测细胞增殖，体外侵袭实验检测细胞侵袭，蛋白质印迹检测Ki67、VEGF、MMP-2和cleaved caspase-3的表达。结果　miR-143-3p mimic抑制人结肠癌细胞SW620中MAPK1 mRNA和蛋白的表达水平；miR-143-3p mimic与MAPK1野生型报告载体共转后，荧光素酶的活性显著降低；miR-143-3p mimic转染SW620细胞后，细胞增殖、侵袭能力显著降低，凋亡细胞数目显著增加，Ki67、VEGF和MMP-2的表达水平显著降低，cl-caspase-3的表达水平显著上升；miR-143-3p mimic能缓解MAPK1高表达对SW620细胞增殖、侵袭的诱导作用和对细胞凋亡的抑制作用。结论　miR-143-3p抑制人结肠癌细胞增殖和侵袭，诱导细胞凋亡，其作用机制与靶向抑制MAPK1有关。

关键词: 结肠癌, 　微小RNA, 　MAPK1, 　增殖, 　凋亡, 　侵袭

Abstract: Objective　To investigate the relationship between miR-143-3p targeting MAPK1 and its effect on proliferation, apoptosis and invasion of human colon cancer cells.　Methods　qRT-PCR was used to detect the effect of transfection and the expression level of MAPK1 mRNA. Double luciferase reporter assay was used to detect the targeting relationship between microRNAs-143-3p and MAPK1. The expression level of MAPK1 protein was detected by Western blot. The proliferation multiple was detected by CCK-8. The proliferation was detected by Hoechst staining. The cell invasion was detected by in vitro invasion test. The expression of Ki67, VEGF，MMP-2 and claspase-3 was detected by Western blot.　Results　miR-143-3p mimic inhibited the expression of MAPK1 mRNA and protein in SW620 cells. miR-143-3p mimic and MAPK1 wild-type reporter plasmid decreased the activity of luciferase significantly. After miR-143-3p mimic transfection, the proliferation and invasion of SW620 cells were significantly decreased, the number of apoptotic cells was significantly increased, the expression of Ki67, VEGF and MMP-2 was significantly decreased, and the expression of cl-caspase-3 was significantly increased. The miR-143-3p mimic alleviated the induction effect of high expression of MAPK1 on proliferation and invasion of SW620 cells and inhibition effect on cell apoptosis.　Conclusion　miR-143-3p can inhibit proliferation and invasion of human colon cancer cells and induce apoptosis through possible targeting inhibition of MAPK1.

Key words: Colon cancer, 　Micro RNA, 　MAPK1, 　Proliferation, 　Apoptosis, 　Invasion

中图分类号:

R735.3

张志谦, 张广星, 彭小东. miR-143-3p 靶向MAPK1对人结肠癌细胞增殖，凋亡和侵袭的调节作用#br#[J]. 中国临床解剖学杂志, 2019, 37(3): 292-298.

ZHANG Zhi-qian, ZHANG Guang-xin, PENG Xiao-dong. The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1[J]. Chinese Journal of Clinical Anatomy, 2019, 37(3): 292-298.

参考文献 20

[1]	Mcguire S. World cancer report 2014. Geneva, switzerland: world health organization, international agency for research on cancer, WHO press, 2015[J]. Adv Nutr, 2016,7(2): 418-419.
[2]	Walter FM, Emery JD, Mendonca S, et al. Symptoms and patient factors associated with longer time to diagnosis for colorectal cancer: results from a prospective cohort study[J]. Br J Cancer, 2016,115(5): 533-541.
[3]	Williams TG, Cubiella J, Griffin SJ, et al. Risk prediction models for colorectal cancer in people with symptoms: a systematic review[J]. BMC Gastroenterol, 2016,16(1): 63.
[4]	Stearns MW Jr, Schottenfeld D. Techniques for the surgical manage ment of colon cancer[J]. Cancer, 1971, 28(1): 165-169.
[5]	Li D, Hu J, Song H, et al. Mir-143-3p targeting lim domain kinase 1 suppresses the progression of triple-negative breast cancer cells[J]. Am J Transl Res, 2017, 9(5): 2276-2285.
[6]	Shi H, Shen H, Xu J, et al. Mir-143-3p suppresses the progression of ovarian cancer[J]. Am J Transl Res, 2018, 10(3): 866-874.
[7]	Bracken CP, Scott HS, Goodall GJ. A network-biology perspective of microrna function and dysfunction in cancer[J]. Nat Rev Genet, 2016, 17(12): 719-732.
[8]	Moridikia A, Mirzaei H, Sahebkar A, et al. Micrornas: Potential candidates for diagnosis and treatment of colorectal cancer[J]. J Cell Physiol, 2018, 233(2): 901-913.
[9]	Yang LH, Xie ZC, He RQ, et al. Identification of the potential targets of mir-143-3p in colorectal cancer through bioinformatics analysis[J]. Int J Clin Exp Med, 2017,10(2): 2188-2203.
[10]	Yang F, Xie YQ, Tang SQ, et al. Mir-143 regulates proliferation and apoptosis of colorectal cancer cells and exhibits altered expression in colorectal cancer tissue[J]. Int J Clin Exp Med, 2015, 8(9): 15308-15312.
[11]	Sun Y, Liu WZ, Liu T, et al. Signaling pathway of mapk/erk in cell proliferation, differentiation, migration, senescence and apoptosis[J]. J Recept Signal Transduct Res, 2015, 35(6): 600-604.
[12]	Burotto M, Chiou VL, Lee JM, et al. The mapk pathway across different malignancies: a new perspective[J]. Cancer, 2014, 120(22): 3446-3456.
[13]	Lei YY, Wang WJ, Mei JH, et al. Mitogen-activated protein kinase signal transduction in solid tumors[J]. Asian Pac J Cancer Pre, 2014, 15(20): 8539-8548.
[14]	Wei WT, Nian XX, Wang SY, et al. Mir-422a inhibits cell proliferation in colorectal cancer by targeting akt1 and mapk1[J]. Cancer Cell Int, 2017, 17(1): 91-103.
[15]	Upadhya D, Ogata M, Reneker LW. Mapk1 is required for establishing the pattern of cell proliferation and for cell survival during lens development[J]. Development, 2013, 140(7): 1573-1582.
[16]	Yiwei T, Hua H, Hui G, et al. Hotair interacting with mapk1 regulates ovarian cancer skov3 cell proliferation, migration, and invasion[J]. Med Sci Monit, 2015, 21(6): 1856-1863.
[17]	Ouyang L, Shi Z, Zhao S, et al. Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis[J]. Cell Prolif, 2012, 45(6): 487-498..
[18]	Shalini S, Dorstyn L, Dawar S, et al. Old, new and emerging functions of caspases[J]. Cell Death Differ, 2015, 22(4): 526-539.
[19]	He Z, Yi J, Liu X, et al. Mir-143-3p functions as a tumor suppressor by regulating cell proliferation, invasion and epithelial–mesenchymal transition by targeting qki-5 in esophageal squamous cell carcinoma[J]. Mol Cancer, 2016, 15(1): 51-68.
[20]	Sun X, Dai G, Yu L, et al. Mir-143-3p inhibits the proliferation, migration and invasion in osteosarcoma by targeting fosl2[J]. Sci Rep, 2018, 8(1): 606-616.

miR-143-3p 靶向MAPK1对人结肠癌细胞增殖，凋亡和侵袭的调节作用#br#

The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1

可视化

摘要/Abstract

引用本文

使用本文

参考文献 20

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	郑锴, 罗秀玲, 张玮豪, 刘宇利, 李玉明, 廖尚高. LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究[J]. 中国临床解剖学杂志, 2023, 41(5): 543-549.
[2]	陈平, 魏雪梅, 谈丽丽. 莫诺苷调控miR-483-5p表达对高糖诱导的人视网膜血管内皮细胞氧化应激和凋亡影响[J]. 中国临床解剖学杂志, 2023, 41(4): 434-439.
[3]	李丽楠, 丁汉琳, 刘金川. miR-181a调控sirt1通路抑制七氟烷诱导的海马神经元凋亡[J]. 中国临床解剖学杂志, 2023, 41(2): 200-206.
[4]	吕艳利, 巴凯, 方政. 微小RNA-3651过表达通过介导核因子κB信号通路抑制人舌癌细胞CAL27的生长与侵袭[J]. 中国临床解剖学杂志, 2023, 41(2): 194-199.
[5]	高山, 庞婕, 李占结, 李晨希, 尚庆毅. 珠子参总皂苷上调miR-216a抑制人巨细胞病毒感染人胚肺成纤维细胞损伤[J]. 中国临床解剖学杂志, 2022, 40(6): 689-695.
[6]	胡伟贤, 王俊江, 吴德庆, 吴伍林, 吕泽坚, 李勇, 蔡观福, 姚学清. 两孔法腹腔镜辅助右半结肠癌完整系膜切除术技术要点及短期疗效[J]. 中国临床解剖学杂志, 2022, 40(2): 228-233.
[7]	马大亮, 崔红莉, 荣卫江, 贾琦, 黄福献. 柴胡皂苷A减轻脑缺血再灌注大鼠海马神经元损伤[J]. 中国临床解剖学杂志, 2021, 39(5): 569-574.
[8]	吴旭玲, 董楝, 彭爽, 叶兰, 徐祖才, 冯占辉. 颞叶外侧癫痫患者脑组织NHE1及凋亡相关蛋白的表达[J]. 中国临床解剖学杂志, 2021, 39(4): 431-436.
[9]	张海燕, 孙丽慧, 潘洪明, 李林, 廉洁, 于晶, 郎尉雅. 曲古抑菌素A通过JNK/MAPK信号通路介导乳腺癌MDA-MB-231细胞的凋亡[J]. 中国临床解剖学杂志, 2021, 39(2): 174-181.
[10]	周兰柱, 赵报, 张明洁, 崔忆旋, 吴俊, 孙哲, 柳申成, 王文忠. miR-146a通过靶向CCNJ调控鼻咽癌细胞HNE-1/DDP对顺铂敏感性的影响[J]. 中国临床解剖学杂志, 2021, 39(1): 55-59.
[11]	邓永红, 张力, 王璟, 颜爱华. 长链非编码RNA PVT1通过靶向miR-190对宫颈癌HeLa细胞生存及转移的影响[J]. 中国临床解剖学杂志, 2020, 38(4): 414-420.
[12]	赵阳, 万银绪, 车吉忠, 徐延凯, 李庆元. miR-128-3p过表达靶向MAPK1抑制膀胱癌5637细胞株的侵袭，迁移和上皮间质转化[J]. 中国临床解剖学杂志, 2019, 37(5): 534-541.
[13]	吴闽, 侯慧珍, 司廷林. 过表达miR-138抑制TCF-4对甲状腺癌细胞CAL-62增殖，凋亡和运动的调节作用[J]. 中国临床解剖学杂志, 2019, 37(4): 425-430.
[14]	陈婕,汤利华,李正明. 高尔基体基质蛋白130通过诱导上皮间充质转化促进甲状腺乳头状癌细胞TPC-1的侵袭和迁移[J]. 中国临床解剖学杂志, 2019, 37(1): 64-70.
[15]	张淑艳,张方,姬颖华. 鼠李素下调Notch-1的表达对乳腺癌MCF-7细胞增殖、侵袭和迁移的调节作用[J]. 中国临床解剖学杂志, 2018, 36(6): 632-636.