关键词: 骨标本, 　真空浸渍, 　环氧滴胶, 　保护措施

Abstract: Objective　This study aims to increase the accessibility and the life spans of bone specimens and to overcome the shortcomings of the polyurethane resin painting method that is widely used in bone specimen preservation.　Methods　Bone specimens of a male donor were first dried, and then immersed into the HZ-806A/HZ-806B two-component transparent epoxy resin for vacuum infusion. Then, the specimens were put into a mixed solution with a ethanol to glycerol ratio of 2:1 in weight for solidifying for 12 hours. After that, all specimens were moved into anhydrous ethanol to remove the glycerol component and then dried in the air. Last, the changes of the compact bone and the spongy bone were evaluated comprehensively with the aspects of appearance, anatomical features and physical properties.　Results　The cured transparent epoxy resin filled the space around bone trabecula in the spongy bone, and formed a hard, glossy, and transparent film on the surface of the bones, improving the hardness, toughness, safety, and color of the bone specimens. According to the statistical results, the average gaining weight rate of all specimens was 80.3%.　Conclusion　The vacuum infusion technique has a prominent advantage in bone processing, especially for the brittle bones that are hard to be utilized. Hopefully, this method will serve as a new common technical means for skeleton preservation.

Key words: Bone specimen, 　Vacuum infiltration, 　Epoxy resin, 　Protective measures