p-PDGFRα在小鼠黑质纹状体损伤周边的经时表达和定位

doi:10.13418/j.issn.1001-165x.2021.01.015

中国临床解剖学杂志 ›› 2021, Vol. 39 ›› Issue (1): 76-81.doi: 10.13418/j.issn.1001-165x.2021.01.015

p-PDGFRα在小鼠黑质纹状体损伤周边的经时表达和定位

裴丹，　刘学

锦州医科大学人体解剖学教研室，辽宁锦州 121000

收稿日期:2019-07-31 出版日期:2021-01-25 发布日期:2021-01-27
作者简介:裴丹（1982-），女，博士，副教授，研究方向：人体解剖与组织胚胎学，中枢神经损伤与修复，E-mail：245369662@qq.com
基金资助:
辽宁省教育厅科学技术研究项目青年项目（JYTQ N201716）

The expression and localization of p-PDGFR alpha at different stages after substantia nigra striatum injury in mice

Pei Dan, Liu Xue

Department of Anatomy, Jinzhou Medical University, Jinzhou 121000, Liaoning Province, China

Received:2019-07-31 Online:2021-01-25 Published:2021-01-27

摘要/Abstract

摘要： 　目的　探讨血小板衍生生长因子受体α（platelet-derived growth factor receptor alpha，PDGFRα）在小鼠黑质纹状体损伤周边的经时表达和定位。方法　取8周龄BALB/c小鼠，按文献制备小鼠黑质纹状体通路损伤模型，分别于术后第1、4、7、14 d取损伤脑组织（延及损伤部位前后2 mm）进行检测。应用免疫组织化学染色和Western blot检测p-PDGFRα蛋白的经时表达；采用免疫荧光双重染色检测p-PDGFRα的定位表达。结果　免疫组化和Western blot检测均显示，损伤后4 d p-PDGFRα表达至高峰水平，免疫荧光双重染色结果显示其表达在星形胶质细胞，小胶质细胞，少突胶质细胞祖细胞和粒细胞上。结论　脑损伤可以使PDGFRα活化并表达于损伤周围，活化的PDGFRα可能与星形胶质细胞，小胶质细胞，少突胶质细胞祖细胞和粒细胞的增殖有关。

关键词: 血小板衍生生长因子受体, 　脑损伤, 　星形胶质细胞, 　小胶质细胞, 　少突胶质细胞祖细胞, 　粒细胞

Abstract: Objective To investigate the expression and localization of platelet-derived growth factor receptor alpha (PDGFRα) at different stages after substantia nigra striatum injury in mice. Methods The substantia nigra striatum pathway was unilaterally transected in 8 weeks old BALB/c mice. According to the method of Baidan, brain tissues of 2 mm around the injury brain were examined on the 1st, 4th, 7th and 14th day after operation. The expression of p-PDGFR alpha protein was detected by immunohistochemical staining and Western blot. Immunofluorescence double staining was used to detect the localized expression of p-PDGFR alpha. Results Immunohistochemical staining and Western blot showed that the expression of p-PDGFR alpha reached its peak level 4 days after injury. Double immunofluorescence staining showed that p-PDGFR alpha was expressed in astrocytes, microglia, leukocytes and oligodendrocytes. Conclusions Brain injury can activate PDGFR alpha and express around the injury. Activated PDGFR alpha may be related to the proliferation of astrocytes, microglia, leukocytes and oligodendrocytes.

Key words: PDGFR, 　Brain injury;　Astrocytes;　Microglia, 　Oligodendrocytes, 　Leukocytes

中图分类号:

R322.81

裴丹, 刘学. p-PDGFRα在小鼠黑质纹状体损伤周边的经时表达和定位[J]. 中国临床解剖学杂志, 2021, 39(1): 76-81.

Pei Dan, Liu Xue. The expression and localization of p-PDGFR alpha at different stages after substantia nigra striatum injury in mice[J]. Chinese Journal of Clinical Anatomy, 2021, 39(1): 76-81.

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p-PDGFRα在小鼠黑质纹状体损伤周边的经时表达和定位

The expression and localization of p-PDGFR alpha at different stages after substantia nigra striatum injury in mice

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